Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture
Abstract To investigate the correlation between metal element levels, AMPK(AMP-activated protein kinase) and NF-κBp65(Nuclear factor kappa-B p65) and PPROM(preterm premature rupture of membranes). 20cases of PPROM,33cases of TPROM(Term premature rupture of membranes), and 33cases of nPROM were selec...
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Nature Portfolio
2025-07-01
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| author | Siqi Lu Yikun Zhao Weibing Chen Yingying Niu Dapeng Wang Zhenai Jin |
| author_facet | Siqi Lu Yikun Zhao Weibing Chen Yingying Niu Dapeng Wang Zhenai Jin |
| author_sort | Siqi Lu |
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| description | Abstract To investigate the correlation between metal element levels, AMPK(AMP-activated protein kinase) and NF-κBp65(Nuclear factor kappa-B p65) and PPROM(preterm premature rupture of membranes). 20cases of PPROM,33cases of TPROM(Term premature rupture of membranes), and 33cases of nPROM were selected, totaling 86patients.Metal element content in umbilical cord blood serum was measured using ICP-MS(Inductively Coupled Plasma Mass Spectrometry).HE(Hematoxylin-eosin) staining was utilized to observe histological changes in the placentas of the three groups.IHC(Immunohistochemistry) and Western Blot were applied to detect the expression levels of AMPKα and NF-κBp65 in the placental tissues. The levels of Mg, Fe, and Mo in the PPROM group were significantly lower than those in the nPROM group and TPROM group(P<0.05).The levels of Al, Cr, and As in the PPROM group were significantly higher than those of the TPROM group(P<0.05).The incidence of chorioamnionitis in the PPROM group was higher than that in the TPROM group and nPROM group(P < 0.05).The positive expression rates of AMPKα and NF-κBp65 in the placental tissues of the PPROM group were lower than those of the TPROM group and the nPROM group(P < 0.05). Exposure to Al, Cr, As during pregnancy or a deficiency in Mg, Fe, Mo may be among the risk factors for PPROM.The decreased expression of AMPK and NF-κBp65 in placental tissue may be one of the mechanisms contributing to the occurrence of PPROM. |
| format | Article |
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| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a99d2aab6cab40ffb9b8b1c2fbaf660f2025-08-20T03:05:17ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-11374-3Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane ruptureSiqi Lu0Yikun Zhao1Weibing Chen2Yingying Niu3Dapeng Wang4Zhenai Jin5Department of Pediatrics, Affiliated Hospital of Yanbian UniversityNeonatology Department, Rizhao People’s Hospital of Jining Medical UniversityNeonatology Department, Rizhao People’s Hospital of Jining Medical UniversityNeonatology Department, Jining First People’s HospitalDepartment of Pediatrics, Affiliated Hospital of Yanbian UniversityDepartment of Pediatrics, Affiliated Hospital of Yanbian UniversityAbstract To investigate the correlation between metal element levels, AMPK(AMP-activated protein kinase) and NF-κBp65(Nuclear factor kappa-B p65) and PPROM(preterm premature rupture of membranes). 20cases of PPROM,33cases of TPROM(Term premature rupture of membranes), and 33cases of nPROM were selected, totaling 86patients.Metal element content in umbilical cord blood serum was measured using ICP-MS(Inductively Coupled Plasma Mass Spectrometry).HE(Hematoxylin-eosin) staining was utilized to observe histological changes in the placentas of the three groups.IHC(Immunohistochemistry) and Western Blot were applied to detect the expression levels of AMPKα and NF-κBp65 in the placental tissues. The levels of Mg, Fe, and Mo in the PPROM group were significantly lower than those in the nPROM group and TPROM group(P<0.05).The levels of Al, Cr, and As in the PPROM group were significantly higher than those of the TPROM group(P<0.05).The incidence of chorioamnionitis in the PPROM group was higher than that in the TPROM group and nPROM group(P < 0.05).The positive expression rates of AMPKα and NF-κBp65 in the placental tissues of the PPROM group were lower than those of the TPROM group and the nPROM group(P < 0.05). Exposure to Al, Cr, As during pregnancy or a deficiency in Mg, Fe, Mo may be among the risk factors for PPROM.The decreased expression of AMPK and NF-κBp65 in placental tissue may be one of the mechanisms contributing to the occurrence of PPROM.https://doi.org/10.1038/s41598-025-11374-3Preterm premature rupture of membraneMetal exposureOxidative stressAMP-activated protein kinaseNuclear factor-κBp65 |
| spellingShingle | Siqi Lu Yikun Zhao Weibing Chen Yingying Niu Dapeng Wang Zhenai Jin Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture Scientific Reports Preterm premature rupture of membrane Metal exposure Oxidative stress AMP-activated protein kinase Nuclear factor-κBp65 |
| title | Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture |
| title_full | Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture |
| title_fullStr | Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture |
| title_full_unstemmed | Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture |
| title_short | Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture |
| title_sort | hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and ampk nf κb signaling in preterm membrane rupture |
| topic | Preterm premature rupture of membrane Metal exposure Oxidative stress AMP-activated protein kinase Nuclear factor-κBp65 |
| url | https://doi.org/10.1038/s41598-025-11374-3 |
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