Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells
Abstract Intervertebral disc degeneration (IVDD) stands as a prevalent chronic orthopedic ailment, profoundly impacting patients' well‐being due to incapacitating low back pain. Studies have highlighted a close correlation between IVDD and the programmed cell death of nucleus pulposus (NP) cell...
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Wiley
2024-12-01
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| Online Access: | https://doi.org/10.1002/jsp2.70020 |
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| author | Xiaoqiang Wang Chao Song Daqian Zhou Yongliang Mei Weiye Cai Rui Chen Jiale Lv Houyin Shi Zongchao Liu |
| author_facet | Xiaoqiang Wang Chao Song Daqian Zhou Yongliang Mei Weiye Cai Rui Chen Jiale Lv Houyin Shi Zongchao Liu |
| author_sort | Xiaoqiang Wang |
| collection | DOAJ |
| description | Abstract Intervertebral disc degeneration (IVDD) stands as a prevalent chronic orthopedic ailment, profoundly impacting patients' well‐being due to incapacitating low back pain. Studies have highlighted a close correlation between IVDD and the programmed cell death of nucleus pulposus (NP) cells orchestrated by interleukin‐1 beta (IL‐1β), tumor necrosis factor‐alpha (TNF‐α), and caspase‐3 (CASP3). Puerarin, renowned for its anti‐inflammatory attributes and its influence on IL‐1β and TNF‐α, emerges as a promising candidate for IVDD treatment. However, the precise mechanism by which it regulates apoptosis via these pathways remains ambiguous. This investigation utilizes bioinformatics to unveil the molecular intricacies of puerarin‐mediated apoptosis regulation in IVDD, substantiated by preliminary in vitro experiments. Analysis exposes aberrant expression of pivotal apoptosis‐associated proteins (IL‐1β, TNF‐α, CASP3, CASP8, and BCL2) in IVDD patients, with network pharmacology indicating puerarin's potential efficacy in IVDD treatment by modulating apoptosis and cellular senescence pathways. Further experiments elucidate puerarin's capacity to stimulate NP cell proliferation while inhibiting apoptosis, potentially contributing to IVDD mitigation. Western blot and PCR outcomes reveal escalated expression of apoptosis‐related proteins (IL‐1β, TNF‐α, and CASP3) in lipopolysaccharide‐treated NPCs, ameliorated by puerarin intervention. Molecular docking simulations demonstrate favorable binding properties of puerarin with apoptotic proteins, while flow cytometry analysis indicates its ability to diminish NPC apoptosis. These discoveries imply that puerarin might alleviate NPC apoptosis by modulating key targets, thereby potentially ameliorating IVDD. In summary, this study unveils the intrinsic mechanism of puerarin in regulating NPC apoptosis to alleviate IVDD, underscoring its therapeutic promise. |
| format | Article |
| id | doaj-art-a98ea7a00ca344b5a976d5f6e2e3364a |
| institution | Kabale University |
| issn | 2572-1143 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj-art-a98ea7a00ca344b5a976d5f6e2e3364a2024-12-26T11:06:34ZengWileyJOR Spine2572-11432024-12-0174n/an/a10.1002/jsp2.70020Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cellsXiaoqiang Wang0Chao Song1Daqian Zhou2Yongliang Mei3Weiye Cai4Rui Chen5Jiale Lv6Houyin Shi7Zongchao Liu8Department of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaDepartment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital Southwest Medical University Luzhou ChinaAbstract Intervertebral disc degeneration (IVDD) stands as a prevalent chronic orthopedic ailment, profoundly impacting patients' well‐being due to incapacitating low back pain. Studies have highlighted a close correlation between IVDD and the programmed cell death of nucleus pulposus (NP) cells orchestrated by interleukin‐1 beta (IL‐1β), tumor necrosis factor‐alpha (TNF‐α), and caspase‐3 (CASP3). Puerarin, renowned for its anti‐inflammatory attributes and its influence on IL‐1β and TNF‐α, emerges as a promising candidate for IVDD treatment. However, the precise mechanism by which it regulates apoptosis via these pathways remains ambiguous. This investigation utilizes bioinformatics to unveil the molecular intricacies of puerarin‐mediated apoptosis regulation in IVDD, substantiated by preliminary in vitro experiments. Analysis exposes aberrant expression of pivotal apoptosis‐associated proteins (IL‐1β, TNF‐α, CASP3, CASP8, and BCL2) in IVDD patients, with network pharmacology indicating puerarin's potential efficacy in IVDD treatment by modulating apoptosis and cellular senescence pathways. Further experiments elucidate puerarin's capacity to stimulate NP cell proliferation while inhibiting apoptosis, potentially contributing to IVDD mitigation. Western blot and PCR outcomes reveal escalated expression of apoptosis‐related proteins (IL‐1β, TNF‐α, and CASP3) in lipopolysaccharide‐treated NPCs, ameliorated by puerarin intervention. Molecular docking simulations demonstrate favorable binding properties of puerarin with apoptotic proteins, while flow cytometry analysis indicates its ability to diminish NPC apoptosis. These discoveries imply that puerarin might alleviate NPC apoptosis by modulating key targets, thereby potentially ameliorating IVDD. In summary, this study unveils the intrinsic mechanism of puerarin in regulating NPC apoptosis to alleviate IVDD, underscoring its therapeutic promise.https://doi.org/10.1002/jsp2.70020apoptosisbioinformaticsintervertebral disc degenerationnucleus pulposus cellspuerarin |
| spellingShingle | Xiaoqiang Wang Chao Song Daqian Zhou Yongliang Mei Weiye Cai Rui Chen Jiale Lv Houyin Shi Zongchao Liu Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells JOR Spine apoptosis bioinformatics intervertebral disc degeneration nucleus pulposus cells puerarin |
| title | Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| title_full | Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| title_fullStr | Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| title_full_unstemmed | Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| title_short | Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| title_sort | exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells |
| topic | apoptosis bioinformatics intervertebral disc degeneration nucleus pulposus cells puerarin |
| url | https://doi.org/10.1002/jsp2.70020 |
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