Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS
Ilicis Rotundae Cortex (IRC) consists of the bark of Ilex rotunda Thunb, and its chemical constituents mainly include flavonoid glycosides, phenols, and triterpenoid saponins. In this study, a preliminary analysis was performed to identify and obtain the chemical components from IRC to better contro...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/9570776 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557046171435008 |
|---|---|
| author | Xinxin Chen Junmao Li Renyikun Yuan Youqiong Zhuo Yangling Chen Chenwei Zhang Ming Chen Hong-Wei Gao Zhenjie Liu Yulin Feng Zheng-Tao Wang Shilin Yang |
| author_facet | Xinxin Chen Junmao Li Renyikun Yuan Youqiong Zhuo Yangling Chen Chenwei Zhang Ming Chen Hong-Wei Gao Zhenjie Liu Yulin Feng Zheng-Tao Wang Shilin Yang |
| author_sort | Xinxin Chen |
| collection | DOAJ |
| description | Ilicis Rotundae Cortex (IRC) consists of the bark of Ilex rotunda Thunb, and its chemical constituents mainly include flavonoid glycosides, phenols, and triterpenoid saponins. In this study, a preliminary analysis was performed to identify and obtain the chemical components from IRC to better control the quality of the medicinal materials and provide a chemical basis for the study of the efficacy of the active components. Simple and efficient sample pretreatment and ultrasonic-assisted extraction methods were used to analyze the mass spectrum fragments and fracture modes in the anion mode by UPLC-Q-TOF-MS/MS. Using a two-step strategy, the neutral loss, diagnostic ions, and characteristic fragments were studied to screen diverse skeletons and substitutions, and the possible compounds were identified by comparison with databases. The representative compounds were compared with the standard, and the mass spectrogram was found to match perfectly. Thus, our findings reveal that this method is feasible and reliable and can be used to analyze the chemical components of IRC. We identified 105 compounds, including 22 triterpenoid saponins, 15 chlorogenic acids, 33 phenylpropanoids and phenylpropanosides, 3 iridoids, 1 flavonoid, 10 lignans, 12 glycosides, and 9 other compounds. This method lays the foundation for further elucidating the pharmacodynamics of IRC and provides a practical method for the identification of IRC. |
| format | Article |
| id | doaj-art-a98837e9a495422fac769fdf37cd53b0 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a98837e9a495422fac769fdf37cd53b02025-02-03T05:43:46ZengWileyJournal of Chemistry2090-90632090-90712021-01-01202110.1155/2021/95707769570776Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MSXinxin Chen0Junmao Li1Renyikun Yuan2Youqiong Zhuo3Yangling Chen4Chenwei Zhang5Ming Chen6Hong-Wei Gao7Zhenjie Liu8Yulin Feng9Zheng-Tao Wang10Shilin Yang11College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaJiangxi University of Chinese Medicine, Nanchang 330002, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaJiangxi University of Chinese Medicine, Nanchang 330002, ChinaShanghai University of Chinese Medicine, Shanghai 201203, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, ChinaIlicis Rotundae Cortex (IRC) consists of the bark of Ilex rotunda Thunb, and its chemical constituents mainly include flavonoid glycosides, phenols, and triterpenoid saponins. In this study, a preliminary analysis was performed to identify and obtain the chemical components from IRC to better control the quality of the medicinal materials and provide a chemical basis for the study of the efficacy of the active components. Simple and efficient sample pretreatment and ultrasonic-assisted extraction methods were used to analyze the mass spectrum fragments and fracture modes in the anion mode by UPLC-Q-TOF-MS/MS. Using a two-step strategy, the neutral loss, diagnostic ions, and characteristic fragments were studied to screen diverse skeletons and substitutions, and the possible compounds were identified by comparison with databases. The representative compounds were compared with the standard, and the mass spectrogram was found to match perfectly. Thus, our findings reveal that this method is feasible and reliable and can be used to analyze the chemical components of IRC. We identified 105 compounds, including 22 triterpenoid saponins, 15 chlorogenic acids, 33 phenylpropanoids and phenylpropanosides, 3 iridoids, 1 flavonoid, 10 lignans, 12 glycosides, and 9 other compounds. This method lays the foundation for further elucidating the pharmacodynamics of IRC and provides a practical method for the identification of IRC.http://dx.doi.org/10.1155/2021/9570776 |
| spellingShingle | Xinxin Chen Junmao Li Renyikun Yuan Youqiong Zhuo Yangling Chen Chenwei Zhang Ming Chen Hong-Wei Gao Zhenjie Liu Yulin Feng Zheng-Tao Wang Shilin Yang Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS Journal of Chemistry |
| title | Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS |
| title_full | Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS |
| title_fullStr | Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS |
| title_full_unstemmed | Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS |
| title_short | Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS |
| title_sort | rapid identification of the chemical components of ilex rotunda thunb using uplc q tof ms ms |
| url | http://dx.doi.org/10.1155/2021/9570776 |
| work_keys_str_mv | AT xinxinchen rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT junmaoli rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT renyikunyuan rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT youqiongzhuo rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT yanglingchen rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT chenweizhang rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT mingchen rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT hongweigao rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT zhenjieliu rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT yulinfeng rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT zhengtaowang rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms AT shilinyang rapididentificationofthechemicalcomponentsofilexrotundathunbusinguplcqtofmsms |