Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system
With the increase in cross-border transmission in the context of globalization, the necessity for developing rapid and accurate detection methods for plant pathogens has become critical. This study introduces a recombinase polymerase amplification (RPA) technique combined with CRISPR/Cas12a cleavage...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2025-06-01
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| Series: | IMA Fungus |
| Online Access: | https://imafungus.pensoft.net/article/153604/download/pdf/ |
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| author | Dorji Phurbu Zhipeng Feng Lei Cai Fang Liu |
| author_facet | Dorji Phurbu Zhipeng Feng Lei Cai Fang Liu |
| author_sort | Dorji Phurbu |
| collection | DOAJ |
| description | With the increase in cross-border transmission in the context of globalization, the necessity for developing rapid and accurate detection methods for plant pathogens has become critical. This study introduces a recombinase polymerase amplification (RPA) technique combined with CRISPR/Cas12a cleavage and fluorescence-based detection systems (FRB) or paper-based lateral flow strips (PLFS) for the rapid on-site detection of invasive alien fungi, specifically Alternaria triticina and Plenodomus libanotidis, which pose significant threats to agriculture and biodiversity. The results demonstrate that either RPA-CRISPR/Cas12a-FRB or RPA-CRISPR/Cas12a-PLFS can accurately detect the target species within 30 min, with a sensitivity of up to 10 pg/μL. These portable and easy-to-use assays are suitable for rapid on-site screening of plant pathogenic fungi in plant tissues, enabling applications in disease control and port quarantine. |
| format | Article |
| id | doaj-art-a987ef3b75ce4a13a24b5000e05ad088 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-a987ef3b75ce4a13a24b5000e05ad0882025-08-20T03:44:47ZengPensoft PublishersIMA Fungus2210-63592025-06-011611710.3897/imafungus.16.153604153604Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a systemDorji Phurbu0Zhipeng Feng1Lei Cai2Fang Liu3Institute of Plateau Biology of Xizang Autonomous RegionChinese Academy of SciencesUniversity of Chinese Academy of SciencesUniversity of Chinese Academy of SciencesWith the increase in cross-border transmission in the context of globalization, the necessity for developing rapid and accurate detection methods for plant pathogens has become critical. This study introduces a recombinase polymerase amplification (RPA) technique combined with CRISPR/Cas12a cleavage and fluorescence-based detection systems (FRB) or paper-based lateral flow strips (PLFS) for the rapid on-site detection of invasive alien fungi, specifically Alternaria triticina and Plenodomus libanotidis, which pose significant threats to agriculture and biodiversity. The results demonstrate that either RPA-CRISPR/Cas12a-FRB or RPA-CRISPR/Cas12a-PLFS can accurately detect the target species within 30 min, with a sensitivity of up to 10 pg/μL. These portable and easy-to-use assays are suitable for rapid on-site screening of plant pathogenic fungi in plant tissues, enabling applications in disease control and port quarantine.https://imafungus.pensoft.net/article/153604/download/pdf/ |
| spellingShingle | Dorji Phurbu Zhipeng Feng Lei Cai Fang Liu Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system IMA Fungus |
| title | Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system |
| title_full | Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system |
| title_fullStr | Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system |
| title_full_unstemmed | Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system |
| title_short | Rapid and sensitive diagnosis of plant quarantine fungi Alternaria triticina and Plenodomus libanotidis based on the RPA-CRISPR/Cas12a system |
| title_sort | rapid and sensitive diagnosis of plant quarantine fungi alternaria triticina and plenodomus libanotidis based on the rpa crispr cas12a system |
| url | https://imafungus.pensoft.net/article/153604/download/pdf/ |
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