The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats
Background: Inflammation plays a major role in the development and progression of diabetes. Vitamin D deficiency and physical inactivity can also increase the risk of developing type 2 diabetes. Combined therapeutic strategies are promising approaches for the treatment and prevention of diabetes. Th...
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Golestan University of Medical Sciences
2025-05-01
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| Series: | Medical Laboratory Journal |
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| Online Access: | http://mlj.goums.ac.ir/article-1-1603-en.pdf |
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| author | Behrooz Mohammadnezhad Seyed Abdollah Hashemvarzi |
| author_facet | Behrooz Mohammadnezhad Seyed Abdollah Hashemvarzi |
| author_sort | Behrooz Mohammadnezhad |
| collection | DOAJ |
| description | Background: Inflammation plays a major role in the development and progression of diabetes. Vitamin D deficiency and physical inactivity can also increase the risk of developing type 2 diabetes. Combined therapeutic strategies are promising approaches for the treatment and prevention of diabetes. This study aimed to investigate the effects of resistance training, vitamin D3 supplementation, and adipose-derived mesenchymal stem cell (MSC) transplantation on tumor necrosis factor-beta levels in the cerebral cortex of diabetic rats.
Methods: Eighty male Wistar rats (Weighing 290±19 g) were randomly divided into 10 groups: healthy control, sham, diabetes, training, vitamin D, MSC, training+vitamin D, MSC+training, MSC+vitamin D, and training+MSC+vitamin D. Training groups were subjected to a resistance training program on a ladder. MSC groups received 1.5 × 106 MSCs, and vitamin D supplementation groups received 1 microgram/kilogram of vitamin D3 eight times. Cortical TNF-β levels and fasting serum glucose levels were measured.
Results: After six weeks, the combination of resistance training with vitamin D3 supplementation and MSC transplantation (P=0.018), as well as the combination of resistance training with MSCs (P=0.024), significantly reduced the diabetes-induced elevation of TNF-β levels.
Conclusion: Resistance training with appropriate intensity, duration, and recovery between exercise sessions, combined with MSC transplantation and vitamin D3 supplementation, has profound anti-inflammatory effects on the cerebral cortex tissue of diabetic rats. This type of intervention, especially the transplantation of MSCs, may be a promising protective strategy against some complications of diabetes. |
| format | Article |
| id | doaj-art-a9878df5da1b4d7d83aa5378126ef9ec |
| institution | Kabale University |
| issn | 2538-4449 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj-art-a9878df5da1b4d7d83aa5378126ef9ec2025-08-25T05:19:00ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492025-05-011933641The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic ratsBehrooz Mohammadnezhad0Seyed Abdollah Hashemvarzi1 Department of Exercise Physiology, Fe.C., Islamic Azad University, Sari, Iran Department of Exercise Physiology, Fe.C., Islamic Azad University, Sari, Iran Background: Inflammation plays a major role in the development and progression of diabetes. Vitamin D deficiency and physical inactivity can also increase the risk of developing type 2 diabetes. Combined therapeutic strategies are promising approaches for the treatment and prevention of diabetes. This study aimed to investigate the effects of resistance training, vitamin D3 supplementation, and adipose-derived mesenchymal stem cell (MSC) transplantation on tumor necrosis factor-beta levels in the cerebral cortex of diabetic rats. Methods: Eighty male Wistar rats (Weighing 290±19 g) were randomly divided into 10 groups: healthy control, sham, diabetes, training, vitamin D, MSC, training+vitamin D, MSC+training, MSC+vitamin D, and training+MSC+vitamin D. Training groups were subjected to a resistance training program on a ladder. MSC groups received 1.5 × 106 MSCs, and vitamin D supplementation groups received 1 microgram/kilogram of vitamin D3 eight times. Cortical TNF-β levels and fasting serum glucose levels were measured. Results: After six weeks, the combination of resistance training with vitamin D3 supplementation and MSC transplantation (P=0.018), as well as the combination of resistance training with MSCs (P=0.024), significantly reduced the diabetes-induced elevation of TNF-β levels. Conclusion: Resistance training with appropriate intensity, duration, and recovery between exercise sessions, combined with MSC transplantation and vitamin D3 supplementation, has profound anti-inflammatory effects on the cerebral cortex tissue of diabetic rats. This type of intervention, especially the transplantation of MSCs, may be a promising protective strategy against some complications of diabetes.http://mlj.goums.ac.ir/article-1-1603-en.pdfresistance trainingvitamin dstem cellsdiabetes mellitusinflammation |
| spellingShingle | Behrooz Mohammadnezhad Seyed Abdollah Hashemvarzi The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats Medical Laboratory Journal resistance training vitamin d stem cells diabetes mellitus inflammation |
| title | The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats |
| title_full | The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats |
| title_fullStr | The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats |
| title_full_unstemmed | The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats |
| title_short | The evaluation of the effect of periodic resistance training along with vitamin D3 consumption and mesenchymal stem cell transplantation on cortical TNF-β levels in streptozotocin-induced diabetic rats |
| title_sort | evaluation of the effect of periodic resistance training along with vitamin d3 consumption and mesenchymal stem cell transplantation on cortical tnf β levels in streptozotocin induced diabetic rats |
| topic | resistance training vitamin d stem cells diabetes mellitus inflammation |
| url | http://mlj.goums.ac.ir/article-1-1603-en.pdf |
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