Glycocalyx cleavage boosts erythrocytes aggregation
Abstract The glycocalyx is a complex layer of carbohydrate and protein molecules that surrounds the cell membrane of many types of mammalian cells. It serves several important functions, including cell adhesion and communication, and maintain cell shape and stability, especially in the case of eryth...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-75012-0 |
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| author | Mehdi Abbasi Min Jin Yazdan Rashidi Lionel Bureau Daria Tsvirkun Chaouqi Misbah |
| author_facet | Mehdi Abbasi Min Jin Yazdan Rashidi Lionel Bureau Daria Tsvirkun Chaouqi Misbah |
| author_sort | Mehdi Abbasi |
| collection | DOAJ |
| description | Abstract The glycocalyx is a complex layer of carbohydrate and protein molecules that surrounds the cell membrane of many types of mammalian cells. It serves several important functions, including cell adhesion and communication, and maintain cell shape and stability, especially in the case of erythrocytes. Alteration of glycocalyx composition represents a cardiovascular health threatening. For example, in diabetes mellitus glycocalyx of erythrocytes and of endothelial cells is known to be impaired, a potential source of blood occlusion in microcirculation, which may lead to blindness, and renal failure of patients. The impact of glycocalyx impairment on erythrocyte aggregation remains a largely unexplored research area. We conduct here in vitro-experiments in microfluidic devices in order to investigate erythrocytes aggregation incubated with amylase, an enzyme that partially breaks down glycocalyx molecules. It is found that incubation of erythrocytes by amylase leads to a dramatic increase of their aggregation and stability and alters the aggregates morphologies. Confocal microscopy analysis reveals a significant degradation of the glycocalyx layer, correlated with enhanced erythrocytes aggregation. An increased erythrocyte aggregation in vivo should affect oxygen and other metabolites delivery to organs and tissues. This study brings new elements about elucidation of microscopic origins of erythrocyte aggregation and their potential impact on cardiovascular pathologies. |
| format | Article |
| id | doaj-art-a98199f72d474e4384e985eb8419e1c4 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a98199f72d474e4384e985eb8419e1c42025-08-20T01:50:38ZengNature PortfolioScientific Reports2045-23222024-10-0114111110.1038/s41598-024-75012-0Glycocalyx cleavage boosts erythrocytes aggregationMehdi Abbasi0Min Jin1Yazdan Rashidi2Lionel Bureau3Daria Tsvirkun4Chaouqi Misbah5Université Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de PhysiqueUniversité Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de PhysiqueDynamics of Fluids Department of Experimental Physics, Saarland UniversityUniversité Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de PhysiqueUniversité Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de PhysiqueUniversité Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de PhysiqueAbstract The glycocalyx is a complex layer of carbohydrate and protein molecules that surrounds the cell membrane of many types of mammalian cells. It serves several important functions, including cell adhesion and communication, and maintain cell shape and stability, especially in the case of erythrocytes. Alteration of glycocalyx composition represents a cardiovascular health threatening. For example, in diabetes mellitus glycocalyx of erythrocytes and of endothelial cells is known to be impaired, a potential source of blood occlusion in microcirculation, which may lead to blindness, and renal failure of patients. The impact of glycocalyx impairment on erythrocyte aggregation remains a largely unexplored research area. We conduct here in vitro-experiments in microfluidic devices in order to investigate erythrocytes aggregation incubated with amylase, an enzyme that partially breaks down glycocalyx molecules. It is found that incubation of erythrocytes by amylase leads to a dramatic increase of their aggregation and stability and alters the aggregates morphologies. Confocal microscopy analysis reveals a significant degradation of the glycocalyx layer, correlated with enhanced erythrocytes aggregation. An increased erythrocyte aggregation in vivo should affect oxygen and other metabolites delivery to organs and tissues. This study brings new elements about elucidation of microscopic origins of erythrocyte aggregation and their potential impact on cardiovascular pathologies.https://doi.org/10.1038/s41598-024-75012-0 |
| spellingShingle | Mehdi Abbasi Min Jin Yazdan Rashidi Lionel Bureau Daria Tsvirkun Chaouqi Misbah Glycocalyx cleavage boosts erythrocytes aggregation Scientific Reports |
| title | Glycocalyx cleavage boosts erythrocytes aggregation |
| title_full | Glycocalyx cleavage boosts erythrocytes aggregation |
| title_fullStr | Glycocalyx cleavage boosts erythrocytes aggregation |
| title_full_unstemmed | Glycocalyx cleavage boosts erythrocytes aggregation |
| title_short | Glycocalyx cleavage boosts erythrocytes aggregation |
| title_sort | glycocalyx cleavage boosts erythrocytes aggregation |
| url | https://doi.org/10.1038/s41598-024-75012-0 |
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