Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella)
Grass carp, a common aquatic species in China, suffer significant annual losses due to infection by the parasitic ciliate Ichthyophthirius multifiliis (Ich), thus necessitating effective prevention and control measures. In the present study, the Ich β-tubulin gene was cloned and inserted into the eu...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424005039 |
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| author | Jiazheng Ye Fangling Liu Siqi Ren Zhuo Cai Kejing Huang Xinrui Wu Yurui Lin Wanqing Tong Qianhui Li Shanjian Zheng |
| author_facet | Jiazheng Ye Fangling Liu Siqi Ren Zhuo Cai Kejing Huang Xinrui Wu Yurui Lin Wanqing Tong Qianhui Li Shanjian Zheng |
| author_sort | Jiazheng Ye |
| collection | DOAJ |
| description | Grass carp, a common aquatic species in China, suffer significant annual losses due to infection by the parasitic ciliate Ichthyophthirius multifiliis (Ich), thus necessitating effective prevention and control measures. In the present study, the Ich β-tubulin gene was cloned and inserted into the eukaryotic expression vector pVAX1 plasmid to construct the Ich β-tubulin DNA vaccine (pVAX1-Bt). After two doses of the pVAX1-Bt vaccine, the relative percentage survival (RPS) reached 70.0 %, whereas the RPS after 2 doses of pVAX1 reached 10.0 %. The β-tubulin antibody, specific antibody IgM, and immune enzymes (MDA, SOD, CAT, ACP, AKP, and cortisol) were tested over13 weeks. The findings revealed a significant enhancement in the vaccinated fish compared to their unvaccinated counterparts, indicating that the vaccine can activate the humoral immune pathway and non-specific immunity in fish, thereby promoting the production of specific antibodies and memory B cells. Collectively, these results indicate that the Ich β-tubulin DNA vaccine has potential in treatment of Ich infection (ichthyophthiriasis). |
| format | Article |
| id | doaj-art-a97264467b6242c09aa94ef6dbd41dc2 |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-a97264467b6242c09aa94ef6dbd41dc22025-08-20T02:51:30ZengElsevierAquaculture Reports2352-51342024-12-013910241510.1016/j.aqrep.2024.102415Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella)Jiazheng Ye0Fangling Liu1Siqi Ren2Zhuo Cai3Kejing Huang4Xinrui Wu5Yurui Lin6Wanqing Tong7Qianhui Li8Shanjian Zheng9College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China; Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, China; Correspondence to: College of Life Sciences, Zhejiang Normal University, Room 203, 10 teaching buildings, 688 Yingbin Avenue, Jinhua 321004, China.Grass carp, a common aquatic species in China, suffer significant annual losses due to infection by the parasitic ciliate Ichthyophthirius multifiliis (Ich), thus necessitating effective prevention and control measures. In the present study, the Ich β-tubulin gene was cloned and inserted into the eukaryotic expression vector pVAX1 plasmid to construct the Ich β-tubulin DNA vaccine (pVAX1-Bt). After two doses of the pVAX1-Bt vaccine, the relative percentage survival (RPS) reached 70.0 %, whereas the RPS after 2 doses of pVAX1 reached 10.0 %. The β-tubulin antibody, specific antibody IgM, and immune enzymes (MDA, SOD, CAT, ACP, AKP, and cortisol) were tested over13 weeks. The findings revealed a significant enhancement in the vaccinated fish compared to their unvaccinated counterparts, indicating that the vaccine can activate the humoral immune pathway and non-specific immunity in fish, thereby promoting the production of specific antibodies and memory B cells. Collectively, these results indicate that the Ich β-tubulin DNA vaccine has potential in treatment of Ich infection (ichthyophthiriasis).http://www.sciencedirect.com/science/article/pii/S2352513424005039Parasite Ichthyophthirius multifiliisDNA vaccineGrass carpβ-tubulinImmunity |
| spellingShingle | Jiazheng Ye Fangling Liu Siqi Ren Zhuo Cai Kejing Huang Xinrui Wu Yurui Lin Wanqing Tong Qianhui Li Shanjian Zheng Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) Aquaculture Reports Parasite Ichthyophthirius multifiliis DNA vaccine Grass carp β-tubulin Immunity |
| title | Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) |
| title_full | Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) |
| title_fullStr | Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) |
| title_full_unstemmed | Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) |
| title_short | Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella) |
| title_sort | preparation and immunological study of ichthyophthirius multifiliis β tubulin dna vaccine in grass carp ctenopharyngodon idella |
| topic | Parasite Ichthyophthirius multifiliis DNA vaccine Grass carp β-tubulin Immunity |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424005039 |
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