EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS

Introduction. Thrombotic complications caused by the tumor and consequences of its treatment are the leading causes of death in cancer patients. The development of a model for the pathology of hemostasis, in particular the excessive pathological clot formation, in the laboratory animals receiving an...

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Main Authors: M. V. Filonova, E. P. Fedorova, T. Yu. Dubskaya, O. V. Neupokoeva, A. A. Churin
Format: Article
Language:Russian
Published: Russian Academy of Sciences, Tomsk National Research Medical Center 2020-07-01
Series:Сибирский онкологический журнал
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Online Access:https://www.siboncoj.ru/jour/article/view/1494
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author M. V. Filonova
E. P. Fedorova
T. Yu. Dubskaya
O. V. Neupokoeva
A. A. Churin
author_facet M. V. Filonova
E. P. Fedorova
T. Yu. Dubskaya
O. V. Neupokoeva
A. A. Churin
author_sort M. V. Filonova
collection DOAJ
description Introduction. Thrombotic complications caused by the tumor and consequences of its treatment are the leading causes of death in cancer patients. The development of a model for the pathology of hemostasis, in particular the excessive pathological clot formation, in the laboratory animals receiving antitumor agents, could help find new pharmacological methods for correcting hemostatic disorders.The purpose of the study was to study the effect of cisplatin on the blood coagulation system in mice and rats.Results. An experiment using outbred mice showed that the levels of PT-INR and aPTT were decreased and the level of fibrinogen was increased on day 10 after administration of cisplatin in the maximum tolerated dose of 10 mg/kg. A significant decrease in the PT-INR and aPTT levels was observed on day 15 after cisplatin injection only in female mice. The cisplatin injection at a dose of 4 mg/kg resulted in a decrease in the PT-INR, and aPTT levels and an increase in fibrinogen concentration on day 10. In rats, a significant decrease in the PT and aPTT levels was observed in both females and males on day 15 after cisplatin injection.Conclusion. A change in the PT and NIR, aPTT levels towards decrease and fibrinogen concentration towards increase indicates the initiation of thrombus formation.
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spelling doaj-art-a9642140aa9840cdbaafdcaabd1412212025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682020-07-0119310911510.21294/1814-4861-2020-19-3-109-115753EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALSM. V. Filonova0E. P. Fedorova1T. Yu. Dubskaya2O. V. Neupokoeva3A. A. Churin4Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State UniversityGoldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of SciencesGoldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of SciencesGoldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of SciencesGoldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State UniversityIntroduction. Thrombotic complications caused by the tumor and consequences of its treatment are the leading causes of death in cancer patients. The development of a model for the pathology of hemostasis, in particular the excessive pathological clot formation, in the laboratory animals receiving antitumor agents, could help find new pharmacological methods for correcting hemostatic disorders.The purpose of the study was to study the effect of cisplatin on the blood coagulation system in mice and rats.Results. An experiment using outbred mice showed that the levels of PT-INR and aPTT were decreased and the level of fibrinogen was increased on day 10 after administration of cisplatin in the maximum tolerated dose of 10 mg/kg. A significant decrease in the PT-INR and aPTT levels was observed on day 15 after cisplatin injection only in female mice. The cisplatin injection at a dose of 4 mg/kg resulted in a decrease in the PT-INR, and aPTT levels and an increase in fibrinogen concentration on day 10. In rats, a significant decrease in the PT and aPTT levels was observed in both females and males on day 15 after cisplatin injection.Conclusion. A change in the PT and NIR, aPTT levels towards decrease and fibrinogen concentration towards increase indicates the initiation of thrombus formation.https://www.siboncoj.ru/jour/article/view/1494cisplatinoutbred miceoutbred ratscoagulative hemostasisthrombus formation
spellingShingle M. V. Filonova
E. P. Fedorova
T. Yu. Dubskaya
O. V. Neupokoeva
A. A. Churin
EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
Сибирский онкологический журнал
cisplatin
outbred mice
outbred rats
coagulative hemostasis
thrombus formation
title EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
title_full EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
title_fullStr EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
title_full_unstemmed EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
title_short EFFECT OF CISPLATIN ON HEMOSTASIS IN EXPERIMENTAL ANIMALS
title_sort effect of cisplatin on hemostasis in experimental animals
topic cisplatin
outbred mice
outbred rats
coagulative hemostasis
thrombus formation
url https://www.siboncoj.ru/jour/article/view/1494
work_keys_str_mv AT mvfilonova effectofcisplatinonhemostasisinexperimentalanimals
AT epfedorova effectofcisplatinonhemostasisinexperimentalanimals
AT tyudubskaya effectofcisplatinonhemostasisinexperimentalanimals
AT ovneupokoeva effectofcisplatinonhemostasisinexperimentalanimals
AT aachurin effectofcisplatinonhemostasisinexperimentalanimals