Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine
The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected fr...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/8012104 |
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| author | Regeen Handal Lulu Qunibi Ibrahim Sahouri Maha Juhari Rula Dawodi Hiyam Marzouqa Musa Hindiyeh |
| author_facet | Regeen Handal Lulu Qunibi Ibrahim Sahouri Maha Juhari Rula Dawodi Hiyam Marzouqa Musa Hindiyeh |
| author_sort | Regeen Handal |
| collection | DOAJ |
| description | The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β–lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58. None were positive for blaOXA-143 and blaOXA-235. In addition, 5.8% and 0% were positive for blaNDM and blaKPC, respectively. Of the 69 isolates, none were positive for the aminoglycoside aphA6 gene while 93% were positive for the aphA1 gene. The acetyltransferases aacC1 and aacA4 genes tested positive in 22% and 13% of the isolates, respectively. The ompA biofilm-producing virulence gene was detected in all isolates. Finally, Multilocus Sequence Typing (MLST) of 13 isolates revealed that more than one strain of A. baumannii was circulating in Palestinian hospitals as results revealed that 7 isolates were of ST208, 2 isolates ST218, 1 isolate ST231, 1 isolate ST348, and 2 new Sequence Types. The detection of these drug resistant pathogens is a reminder of the importance of active surveillance for resistant bacteria in order to prevent their spread in hospital settings. |
| format | Article |
| id | doaj-art-a95b3de5df384d789db9d2c83c2d2705 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-a95b3de5df384d789db9d2c83c2d27052025-02-03T01:26:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/80121048012104Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in PalestineRegeen Handal0Lulu Qunibi1Ibrahim Sahouri2Maha Juhari3Rula Dawodi4Hiyam Marzouqa5Musa Hindiyeh6Caritas Baby Hospital, Bethlehem, State of PalestineAlia Hospital, Hebron, State of PalestineHussein Hospital, Bethlehem, State of PalestineRafidia Hospital, Nablus, State of PalestineCaritas Baby Hospital, Bethlehem, State of PalestineCaritas Baby Hospital, Bethlehem, State of PalestineCaritas Baby Hospital, Bethlehem, State of PalestineThe American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β–lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58. None were positive for blaOXA-143 and blaOXA-235. In addition, 5.8% and 0% were positive for blaNDM and blaKPC, respectively. Of the 69 isolates, none were positive for the aminoglycoside aphA6 gene while 93% were positive for the aphA1 gene. The acetyltransferases aacC1 and aacA4 genes tested positive in 22% and 13% of the isolates, respectively. The ompA biofilm-producing virulence gene was detected in all isolates. Finally, Multilocus Sequence Typing (MLST) of 13 isolates revealed that more than one strain of A. baumannii was circulating in Palestinian hospitals as results revealed that 7 isolates were of ST208, 2 isolates ST218, 1 isolate ST231, 1 isolate ST348, and 2 new Sequence Types. The detection of these drug resistant pathogens is a reminder of the importance of active surveillance for resistant bacteria in order to prevent their spread in hospital settings.http://dx.doi.org/10.1155/2017/8012104 |
| spellingShingle | Regeen Handal Lulu Qunibi Ibrahim Sahouri Maha Juhari Rula Dawodi Hiyam Marzouqa Musa Hindiyeh Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine International Journal of Microbiology |
| title | Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine |
| title_full | Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine |
| title_fullStr | Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine |
| title_full_unstemmed | Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine |
| title_short | Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine |
| title_sort | characterization of carbapenem resistant acinetobacter baumannii strains isolated from hospitalized patients in palestine |
| url | http://dx.doi.org/10.1155/2017/8012104 |
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