Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate
Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design wit...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/451652 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850160588305465344 |
|---|---|
| author | Vijay Kumar Garlapati Annapurna Kumari Paramita Mahapatra Rintu Banerjee |
| author_facet | Vijay Kumar Garlapati Annapurna Kumari Paramita Mahapatra Rintu Banerjee |
| author_sort | Vijay Kumar Garlapati |
| collection | DOAJ |
| description | Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, Km and Vmax were found to be 310 mM and 0.10 mmol h−1 g−1, respectively. |
| format | Article |
| id | doaj-art-a95a6c42359b44bf83d9c332269c7dc0 |
| institution | OA Journals |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a95a6c42359b44bf83d9c332269c7dc02025-08-20T02:23:07ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/451652451652Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl AcetateVijay Kumar Garlapati0Annapurna Kumari1Paramita Mahapatra2Rintu Banerjee3Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, IndiaMicrobial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, IndiaSolvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, Km and Vmax were found to be 310 mM and 0.10 mmol h−1 g−1, respectively.http://dx.doi.org/10.1155/2013/451652 |
| spellingShingle | Vijay Kumar Garlapati Annapurna Kumari Paramita Mahapatra Rintu Banerjee Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate Journal of Chemistry |
| title | Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate |
| title_full | Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate |
| title_fullStr | Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate |
| title_full_unstemmed | Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate |
| title_short | Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate |
| title_sort | modeling simulation and kinetic studies of solvent free biosynthesis of benzyl acetate |
| url | http://dx.doi.org/10.1155/2013/451652 |
| work_keys_str_mv | AT vijaykumargarlapati modelingsimulationandkineticstudiesofsolventfreebiosynthesisofbenzylacetate AT annapurnakumari modelingsimulationandkineticstudiesofsolventfreebiosynthesisofbenzylacetate AT paramitamahapatra modelingsimulationandkineticstudiesofsolventfreebiosynthesisofbenzylacetate AT rintubanerjee modelingsimulationandkineticstudiesofsolventfreebiosynthesisofbenzylacetate |