Establishment of a Highly Efficient In Vitro Regeneration System for <i>Nandina domestica</i> ‘Firepower’
<i>Nandina domestica</i> ‘Firepower’ is one of the most popular colorful foliage species in landscaping. However, it is currently propagated mainly by seeding and cuttings, with a low reproduction coefficient, hindering the cultivation of this species. Therefore, establishing an in vitro...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/3/421 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Nandina domestica</i> ‘Firepower’ is one of the most popular colorful foliage species in landscaping. However, it is currently propagated mainly by seeding and cuttings, with a low reproduction coefficient, hindering the cultivation of this species. Therefore, establishing an in vitro regeneration system would be beneficial for the industrialized production of <i>Nandina domestica</i> ‘Firepower’. In this study, an ex vivo regeneration system was established using the direct organogenesis pathway. In early April, the new shoots of <i>Nandina domestica</i> ‘Firepower’ were selected, and the stem segments of 1~2 cm were cut as the disinfection materials for the explants. The optimal formulation for inducing axillary shoots was 1/2 MS + 1.5 mg L<sup>−1</sup> 6-benzylaminopurine (BA) + 0.3 mg L<sup>−1</sup> indole-3-butric acid (IBA). The optimal formulation for the differentiation and proliferation of axillary shoots was 1/2 MS + 1.5 mg L<sup>−1</sup> BA + 0.01 mg L<sup>−1</sup> IBA with a multiplicity of proliferation of 9.22. We determined that the rooting of axillary shoots required a combination of IBA, naphthalene acetic acid (NAA), and activated carbon (AC). The optimal formulation for rooting was 1/2 MS + 0.2 mg L<sup>−1</sup> NAA + 0.3 mg L<sup>−1</sup> IBA + 0.2 mg L<sup>−1</sup> AC. After a two-day hardening period for tissue-cultured plantlets, a substrate consisting of peat soil, vermiculite, and perlite at a ratio of 2:2:1 was determined to be the optimal cultivation formulation. This system provides a framework for the industrialized production of <i>Nandina domestica</i> ‘Firepower’. |
|---|---|
| ISSN: | 2223-7747 |