Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation

Surgical meshes have become the standard procedure for a variety of surgical applications with 20 million meshes being implanted each year. The popularity of mesh usage among surgeons is backed by the multiple studies that support its functionality as a tool for improving surgical outcomes. However,...

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Main Authors: Ethan Kallick, Laura Nistico, Mark Longwell, Barbara Byers, Frank Cartieri, Rachael Kreft, Howard Edington
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:International Journal of Biomaterials
Online Access:http://dx.doi.org/10.1155/2019/1063643
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author Ethan Kallick
Laura Nistico
Mark Longwell
Barbara Byers
Frank Cartieri
Rachael Kreft
Howard Edington
author_facet Ethan Kallick
Laura Nistico
Mark Longwell
Barbara Byers
Frank Cartieri
Rachael Kreft
Howard Edington
author_sort Ethan Kallick
collection DOAJ
description Surgical meshes have become the standard procedure for a variety of surgical applications with 20 million meshes being implanted each year. The popularity of mesh usage among surgeons is backed by the multiple studies that support its functionality as a tool for improving surgical outcomes. However, their use has also been associated with infectious surgical complications and many surgeons have turned to biologic meshes. While there have been several studies investigating synthetic meshes, there is limited data comparing synthetic and biologic meshes in vitro in an infection model. This study evaluates the in vitro susceptibility of both synthetic and biologic meshes to single-species methicillin-resistant Staphylococcus aureus (MRSA) biofilms. This research compares biofilm biomass, average thickness, and coverage between the three meshes through florescent in situ hybridization (FISH), confocal scanning microscopy (CSLM), and image analysis. We also report the varying levels of planktonic and attached bacteria through sonication and cfu counts. While the data illustrates increased biofilm formation on biologic mesh in vitro, the study must further be investigated in vivo to confirm the study observations.
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institution Kabale University
issn 1687-8787
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language English
publishDate 2019-01-01
publisher Wiley
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series International Journal of Biomaterials
spelling doaj-art-a9504f0a3f774450aedf1845645c7c0f2025-02-03T01:23:34ZengWileyInternational Journal of Biomaterials1687-87871687-87952019-01-01201910.1155/2019/10636431063643Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro InvestigationEthan Kallick0Laura Nistico1Mark Longwell2Barbara Byers3Frank Cartieri4Rachael Kreft5Howard Edington6Department of Surgery, Allegheny Health Network, Pittsburgh, PA, USACenter for Excellence in Biofilm Research, Allegheny Health Network Research Institute, Pittsburgh, PA, USACenter for Excellence in Biofilm Research, Allegheny Health Network Research Institute, Pittsburgh, PA, USACenter for Excellence in Biofilm Research, Allegheny Health Network Research Institute, Pittsburgh, PA, USADepartment of Surgery, Allegheny Health Network, Pittsburgh, PA, USACenter for Excellence in Biofilm Research, Allegheny Health Network Research Institute, Pittsburgh, PA, USADepartment of Surgery, Allegheny Health Network, Pittsburgh, PA, USASurgical meshes have become the standard procedure for a variety of surgical applications with 20 million meshes being implanted each year. The popularity of mesh usage among surgeons is backed by the multiple studies that support its functionality as a tool for improving surgical outcomes. However, their use has also been associated with infectious surgical complications and many surgeons have turned to biologic meshes. While there have been several studies investigating synthetic meshes, there is limited data comparing synthetic and biologic meshes in vitro in an infection model. This study evaluates the in vitro susceptibility of both synthetic and biologic meshes to single-species methicillin-resistant Staphylococcus aureus (MRSA) biofilms. This research compares biofilm biomass, average thickness, and coverage between the three meshes through florescent in situ hybridization (FISH), confocal scanning microscopy (CSLM), and image analysis. We also report the varying levels of planktonic and attached bacteria through sonication and cfu counts. While the data illustrates increased biofilm formation on biologic mesh in vitro, the study must further be investigated in vivo to confirm the study observations.http://dx.doi.org/10.1155/2019/1063643
spellingShingle Ethan Kallick
Laura Nistico
Mark Longwell
Barbara Byers
Frank Cartieri
Rachael Kreft
Howard Edington
Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
International Journal of Biomaterials
title Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
title_full Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
title_fullStr Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
title_full_unstemmed Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
title_short Resistance of Synthetic and Biologic Surgical Meshes to Methicillin-Resistant Staphylococcus aureus Biofilm: An In Vitro Investigation
title_sort resistance of synthetic and biologic surgical meshes to methicillin resistant staphylococcus aureus biofilm an in vitro investigation
url http://dx.doi.org/10.1155/2019/1063643
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