ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT
ABSTRACT Escherichia coli was isolated from 56 dog fecal sample (69.14%) out of 81 collected samples. The antibacterial activity of E. coli isolate was primarily and secondarily screened on sold and liquid media. The results of primary screening showed that 56 isolates had antibacterial activity ag...
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College of Veterinary Medicine, University of Basrah, Iraq
2010-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_55126_02eaa47dfe1feecac4e4d598304a6326.pdf |
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| _version_ | 1849321450160259072 |
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| author | Murtakab Y. Alhejjaj |
| author_facet | Murtakab Y. Alhejjaj |
| author_sort | Murtakab Y. Alhejjaj |
| collection | DOAJ |
| description | ABSTRACT
Escherichia coli was isolated from 56 dog fecal sample (69.14%) out of 81 collected samples. The antibacterial activity of E. coli isolate was primarily and secondarily screened on sold and liquid media. The results of primary screening showed that 56 isolates had antibacterial activity against tested gram positive and negative standard strains. By secondary screening E. coli isolate was able to produce antimicrobial agent in fermentation media at different incubation conditions, but larger amount of produced antimicrobial agent was obtained at 37Co, 24h, 180rpm by used seed and fermentation media. Different methods were used to determine the purity and to characterize the produced antimicrobial agent physically, chemically and biologically. The results of purity test revealed that the produced antimicrobial agent appeared as pure compound with only one spot in paper chromatography. The result of physical, chemical and biological characterization showed that the produced antimicrobial agent was peptide material with low toxicity 2350mgkg body weight. |
| format | Article |
| id | doaj-art-a94befc5afae4ec8a1292641dae8cb6c |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2010-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-a94befc5afae4ec8a1292641dae8cb6c2025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562010-06-019110111110.33762/bvetr.2010.5512655126ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENTMurtakab Y. AlhejjajABSTRACT Escherichia coli was isolated from 56 dog fecal sample (69.14%) out of 81 collected samples. The antibacterial activity of E. coli isolate was primarily and secondarily screened on sold and liquid media. The results of primary screening showed that 56 isolates had antibacterial activity against tested gram positive and negative standard strains. By secondary screening E. coli isolate was able to produce antimicrobial agent in fermentation media at different incubation conditions, but larger amount of produced antimicrobial agent was obtained at 37Co, 24h, 180rpm by used seed and fermentation media. Different methods were used to determine the purity and to characterize the produced antimicrobial agent physically, chemically and biologically. The results of purity test revealed that the produced antimicrobial agent appeared as pure compound with only one spot in paper chromatography. The result of physical, chemical and biological characterization showed that the produced antimicrobial agent was peptide material with low toxicity 2350mgkg body weight.https://bjvr.uobasrah.edu.iq/article_55126_02eaa47dfe1feecac4e4d598304a6326.pdfescherichiaantibacterial activityfermentation |
| spellingShingle | Murtakab Y. Alhejjaj ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT Basrah Journal of Veterinary Research escherichia antibacterial activity fermentation |
| title | ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT |
| title_full | ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT |
| title_fullStr | ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT |
| title_full_unstemmed | ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT |
| title_short | ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM DOG'S FECES AND STUDY OF THE CHARACTERISTIC PROPERTIES OF IT'S PRODUCED ANTIMICROBIAL AGENT |
| title_sort | isolation and identification of escherichia coli from dog s feces and study of the characteristic properties of it s produced antimicrobial agent |
| topic | escherichia antibacterial activity fermentation |
| url | https://bjvr.uobasrah.edu.iq/article_55126_02eaa47dfe1feecac4e4d598304a6326.pdf |
| work_keys_str_mv | AT murtakabyalhejjaj isolationandidentificationofescherichiacolifromdogsfecesandstudyofthecharacteristicpropertiesofitsproducedantimicrobialagent |