Anti-inflammatory effects of Andrographis paniculata (Fah Talai Jone) via TNFα-JNK pathway and bioactive compound identification
Background: Andrographis paniculata (Fah Talai Jone), a widely used medicinal plant in traditional medicine, is known for its anti-inflammatory properties. This study investigates its bioactive compounds and their role in alleviating inflammation through the TNF-α-JNK pathway. Purpose: The aim of th...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324001945 |
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| Summary: | Background: Andrographis paniculata (Fah Talai Jone), a widely used medicinal plant in traditional medicine, is known for its anti-inflammatory properties. This study investigates its bioactive compounds and their role in alleviating inflammation through the TNF-α-JNK pathway. Purpose: The aim of this study was to identify bioactive compounds in A. paniculata extracts and evaluate their anti-inflammatory effects, focusing on the TNF-α-JNK pathway. Study Design: Experimental research was conducted to assess the antioxidant, cytotoxic and anti-inflammatory properties of both ethanol (APE) and aqueous (APA) extracts of A. paniculata using in vitro methods. Methods: Ethanol (APE) and aqueous (APA) extracts of A. paniculata were prepared and analyzed for total phenolic and flavonoid content, antioxidant activity, and bioactive compound identification using high-performance liquid chromatography (HPLC). Cytotoxicity was evaluated using the sulforhodamine B assay, and the anti-inflammatory effects were assessed through cellular assays measuring reactive oxygen species (ROS) production and JNK pathway modulation. Results: HPLC analysis identified andrographolide as the primary bioactive compound in APE (186.6 ± 2.53 µg/mg). APE exhibited higher phenolic (8.66 ± 0.37 mg GAE/g) and flavonoid (156.33 ± 4.76 mg QE/g) content compared to APA. Antioxidant assays revealed IC50 values of 11.14 ± 1.07 µg/ml for APA and 2.29 ± 0.13 µg/ml for APE. Cytotoxicity studies demonstrated that both extracts were non-toxic to lung cells. APE significantly reduced ROS production (68.3 ± 7.64 % at 62.5 µg/ml) and decreased JNK phosphorylation, indicating its anti-inflammatory potential. Conclusion: Andrographis paniculata, particularly its ethanol extract, exhibits potent antioxidant and anti-inflammatory effects mediated by andrographolide. These findings support its potential as a natural therapeutic agent for managing inflammation. |
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| ISSN: | 2667-0313 |