Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products
A QuEChERS-UPLC-MS/MS method was developed and validated for simultaneous determination of six pesticides (chlorpyrifos, malathion, carbofuran, thiodicarb, deltamethrin, and lambda-cyhalothrin) in dairy products. Samples (n=50), collected from a supermarket in Chongqing, China, were extracted with 1...
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Taylor & Francis Group
2025-12-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2025.2504526 |
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| author | Zhou Lu Zhao Junpeng Hilary Kwesi Ketemepi |
| author_facet | Zhou Lu Zhao Junpeng Hilary Kwesi Ketemepi |
| author_sort | Zhou Lu |
| collection | DOAJ |
| description | A QuEChERS-UPLC-MS/MS method was developed and validated for simultaneous determination of six pesticides (chlorpyrifos, malathion, carbofuran, thiodicarb, deltamethrin, and lambda-cyhalothrin) in dairy products. Samples (n=50), collected from a supermarket in Chongqing, China, were extracted with 1% acetic acid–acetonitrile, purified using PSA, and quantified by external-standard UPLC-MS/MS. All pesticides showed excellent linearity (0.001–0.50 mg/L, R² > 0.99), with LOQs of 0.002 mg/kg. Average recoveries at four spiking levels (0.002, 0.01, 0.1, 1.0 mg/kg) ranged from 89% to 110% (RSD 0.5–9.5%, n=5). Analysis of real samples revealed residues below China’s MRLs (GB 2763–2021), confirming regulatory compliance. The method meets analytical requirements for dairy matrices and offers a reliable framework adaptable to other international standards. |
| format | Article |
| id | doaj-art-a94584707d70414baf07f332b567dcaa |
| institution | OA Journals |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-a94584707d70414baf07f332b567dcaa2025-08-20T01:50:23ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452025-12-0123110.1080/19476337.2025.2504526Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy productsZhou Lu0Zhao Junpeng1Hilary Kwesi Ketemepi2Culinary and Food School, Chongqing Business Vocational College, Chongqing, ChinaTechnology Management Department, Tangshan Food and Drug Comprehensive Testing Center, Tangshan, ChinaFaculty of Food Science and Nutrition, University Malaysia Sabah, Kota Kinabalu, MalaysiaA QuEChERS-UPLC-MS/MS method was developed and validated for simultaneous determination of six pesticides (chlorpyrifos, malathion, carbofuran, thiodicarb, deltamethrin, and lambda-cyhalothrin) in dairy products. Samples (n=50), collected from a supermarket in Chongqing, China, were extracted with 1% acetic acid–acetonitrile, purified using PSA, and quantified by external-standard UPLC-MS/MS. All pesticides showed excellent linearity (0.001–0.50 mg/L, R² > 0.99), with LOQs of 0.002 mg/kg. Average recoveries at four spiking levels (0.002, 0.01, 0.1, 1.0 mg/kg) ranged from 89% to 110% (RSD 0.5–9.5%, n=5). Analysis of real samples revealed residues below China’s MRLs (GB 2763–2021), confirming regulatory compliance. The method meets analytical requirements for dairy matrices and offers a reliable framework adaptable to other international standards.https://www.tandfonline.com/doi/10.1080/19476337.2025.2504526QuEChERSultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)dairy productspesticide residuesfood safety |
| spellingShingle | Zhou Lu Zhao Junpeng Hilary Kwesi Ketemepi Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products CyTA - Journal of Food QuEChERS ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) dairy products pesticide residues food safety |
| title | Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products |
| title_full | Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products |
| title_fullStr | Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products |
| title_full_unstemmed | Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products |
| title_short | Development and validation of a QuEChERS-UPLC-MS/MS method for multi-class pesticide residue analysis in dairy products |
| title_sort | development and validation of a quechers uplc ms ms method for multi class pesticide residue analysis in dairy products |
| topic | QuEChERS ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) dairy products pesticide residues food safety |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2025.2504526 |
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