Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle
Abstract Handmade cloning (HMC) presents several advantages over conventional cloning, including higher throughput, cost-efficiency, and operational simplicity. However, a comprehensive analysis comparing embryo production rates and pregnancy outcomes in cattle has yet to be conducted. This study re...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-96511-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850042057226190848 |
|---|---|
| author | Rina Su Fangrong Ding Moqir Sorgog Ling Li Suyilatu Bai Xiji Ritu Narenhua Nasenochir Ruiwen Fan Muren Herrid |
| author_facet | Rina Su Fangrong Ding Moqir Sorgog Ling Li Suyilatu Bai Xiji Ritu Narenhua Nasenochir Ruiwen Fan Muren Herrid |
| author_sort | Rina Su |
| collection | DOAJ |
| description | Abstract Handmade cloning (HMC) presents several advantages over conventional cloning, including higher throughput, cost-efficiency, and operational simplicity. However, a comprehensive analysis comparing embryo production rates and pregnancy outcomes in cattle has yet to be conducted. This study reveals that cytoplasts produced using the micropipette method exhibited higher quality than those created with a microblade, leading to improved cleavage and blastocyst rates. The fusion of one or double cytoplast did not significantly affect the developmental potential of reconstructed embryos, although blastocysts from single cytoplasts contained slightly fewer cells. Notably, HMC demonstrated higher pregnancy rates and live calf production efficiency compared to conventional cloning. Specifically, for 41 vitrified embryos from conventional cloning, the one-month post-transfer pregnancy rate was 41.4%, resulting in 6 healthy calves (14.6%). In contrast, HMC produced one-month pregnancy rates of 71.4% for fresh and 60.0% for vitrified embryos, yielding 6 (28.5%) and 4 (26.7%) healthy calves, respectively. The optimization of HMC emphasizes the micropipette method’s potential for cattle cloning applications, including genomic selection and gene editing. Additionally, novel insights into the incompatibility issues between nuclear DNA and mitochondrial DNA (mtDNA) in cloned embryos are discussed. |
| format | Article |
| id | doaj-art-a93c61cc0e8042ea89aff30ce0e02401 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a93c61cc0e8042ea89aff30ce0e024012025-08-20T02:55:38ZengNature PortfolioScientific Reports2045-23222025-04-0115111110.1038/s41598-025-96511-8Optimization of handmade cloning enhances pregnancy rates and live calf production in cattleRina Su0Fangrong Ding1Moqir Sorgog2Ling Li3Suyilatu Bai4Xiji Ritu5Narenhua Nasenochir6Ruiwen Fan7Muren Herrid8Grassland & Cattle Investment Co., Ltd.College of Biological Sciences, Chinese Agricultural UniversityGrassland & Cattle Investment Co., Ltd.College of Biological Sciences, Chinese Agricultural UniversityGrassland & Cattle Investment Co., Ltd.Grassland & Cattle Investment Co., Ltd.College of Animal Science, Inner Mongolia Agriculture UniversityCollege of Veterinary Medicine, Shanxi Agricultural UniversityGrassland & Cattle Investment Co., Ltd.Abstract Handmade cloning (HMC) presents several advantages over conventional cloning, including higher throughput, cost-efficiency, and operational simplicity. However, a comprehensive analysis comparing embryo production rates and pregnancy outcomes in cattle has yet to be conducted. This study reveals that cytoplasts produced using the micropipette method exhibited higher quality than those created with a microblade, leading to improved cleavage and blastocyst rates. The fusion of one or double cytoplast did not significantly affect the developmental potential of reconstructed embryos, although blastocysts from single cytoplasts contained slightly fewer cells. Notably, HMC demonstrated higher pregnancy rates and live calf production efficiency compared to conventional cloning. Specifically, for 41 vitrified embryos from conventional cloning, the one-month post-transfer pregnancy rate was 41.4%, resulting in 6 healthy calves (14.6%). In contrast, HMC produced one-month pregnancy rates of 71.4% for fresh and 60.0% for vitrified embryos, yielding 6 (28.5%) and 4 (26.7%) healthy calves, respectively. The optimization of HMC emphasizes the micropipette method’s potential for cattle cloning applications, including genomic selection and gene editing. Additionally, novel insights into the incompatibility issues between nuclear DNA and mitochondrial DNA (mtDNA) in cloned embryos are discussed.https://doi.org/10.1038/s41598-025-96511-8 |
| spellingShingle | Rina Su Fangrong Ding Moqir Sorgog Ling Li Suyilatu Bai Xiji Ritu Narenhua Nasenochir Ruiwen Fan Muren Herrid Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle Scientific Reports |
| title | Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| title_full | Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| title_fullStr | Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| title_full_unstemmed | Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| title_short | Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| title_sort | optimization of handmade cloning enhances pregnancy rates and live calf production in cattle |
| url | https://doi.org/10.1038/s41598-025-96511-8 |
| work_keys_str_mv | AT rinasu optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT fangrongding optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT moqirsorgog optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT lingli optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT suyilatubai optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT xijiritu optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT narenhuanasenochir optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT ruiwenfan optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle AT murenherrid optimizationofhandmadecloningenhancespregnancyratesandlivecalfproductionincattle |