Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer

Abstract Background Extensive animal production systems, such as reindeer husbandry may represent a system to further study the context dependence of stress responses and the potential implications for animal welfare as research on food animal stress and welfare has so far primarily focused on anima...

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Main Authors: Sebastian G. Vetter-Lang, Nikolaus Huber, Leif Egil Loe, Alina L. Evans, Jouko Kumpula, Per Medbøe Thorsby, Erik Ropstad, L. Monica Trondrud
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Veterinary Research
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Online Access:https://doi.org/10.1186/s12917-025-04718-8
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author Sebastian G. Vetter-Lang
Nikolaus Huber
Leif Egil Loe
Alina L. Evans
Jouko Kumpula
Per Medbøe Thorsby
Erik Ropstad
L. Monica Trondrud
author_facet Sebastian G. Vetter-Lang
Nikolaus Huber
Leif Egil Loe
Alina L. Evans
Jouko Kumpula
Per Medbøe Thorsby
Erik Ropstad
L. Monica Trondrud
author_sort Sebastian G. Vetter-Lang
collection DOAJ
description Abstract Background Extensive animal production systems, such as reindeer husbandry may represent a system to further study the context dependence of stress responses and the potential implications for animal welfare as research on food animal stress and welfare has so far primarily focused on animals in intensive animal production systems while animals from extensive production systems, such as reindeer, are yet underrepresented. We investigated short- and longer-term stress responses to repeated herding, handling and restraint and its potential effect on animal welfare in semi-domesticated adult female reindeer (Rangifer tarandus tarandus). We also assessed seasonal differences and the potential effect of the additional stressor of calf removal using serum concentrations of glucocorticoids (cortisol, cortisone and corticosterone), their precursors (11-desoxcortisol, 17-α-hydroxyprogesterone and deoxycorticosterone) and catecholamine metabolites (metanephrine and normetanephrine) in combination with the immunological stress proxy leukocyte coping capacity (LCC) and rectal temperature. Additionally, we assessed the interconnections among different stress indices and their suitability as stress indicators to evaluate handling-induced stress in reindeer, where rectal temperature, other than serum cortisol levels, emerged as a robust and integrative stress parameter. Results Herding, handling, and restraint elicited a marked and seasonally different short-term stress response with higher stress mediator levels in winter. Further, females who had their calf removed shortly after parturition showed increased stress levels based on LCC. The repeated exposure to the same stressors led to a habituation, with decreasing levels of stress indices to the procedure in both seasons. This outcome implies that reindeer females in the present study were able to cope well with repeated manipulations and that this intensification may not compromise animal welfare. Notably, the traditional stress index body temperature correlated with various stress indices encompassing the HPA axis response (cortisol and corticosterone in summer and additionally cortisone and 11-deoxycortisol in winter), the sympathetic-adrenal-medullary system (metanephrine) as well as the immunological response to stress (LCC), in both seasons. Conclusion Our results emphasise body (rectal) temperature as a robust and integrative stress parameter in the context of our study. Our findings add to a foundation for evaluating available stress indices in different individual and environmental contexts and may contribute to improved animal management practices aimed at reducing stress levels and enhancing animal welfare.
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spelling doaj-art-a9383d5daad84e3abfcbaa47a08725a72025-08-20T02:28:08ZengBMCBMC Veterinary Research1746-61482025-04-0121111510.1186/s12917-025-04718-8Getting used to it? Stress of repeated management procedures in semi-domesticated reindeerSebastian G. Vetter-Lang0Nikolaus Huber1Leif Egil Loe2Alina L. Evans3Jouko Kumpula4Per Medbøe Thorsby5Erik Ropstad6L. Monica Trondrud7Centre for Food Science and Veterinary Public Health, University of Veterinary Medicine ViennaCentre for Food Science and Veterinary Public Health, University of Veterinary Medicine ViennaFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life SciencesDepartment of Forestry and Wildlife Management, Inland Norway University of Applied SciencesNatural Resources Institute Finland (Luke)Hormone Laboratory, Department of Medical Biochemistry and Biochemical Endocrinology and Metabolism Research Group, Oslo University HospitalFaculty of Veterinary Science, University of Life SciencesFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life SciencesAbstract Background Extensive animal production systems, such as reindeer husbandry may represent a system to further study the context dependence of stress responses and the potential implications for animal welfare as research on food animal stress and welfare has so far primarily focused on animals in intensive animal production systems while animals from extensive production systems, such as reindeer, are yet underrepresented. We investigated short- and longer-term stress responses to repeated herding, handling and restraint and its potential effect on animal welfare in semi-domesticated adult female reindeer (Rangifer tarandus tarandus). We also assessed seasonal differences and the potential effect of the additional stressor of calf removal using serum concentrations of glucocorticoids (cortisol, cortisone and corticosterone), their precursors (11-desoxcortisol, 17-α-hydroxyprogesterone and deoxycorticosterone) and catecholamine metabolites (metanephrine and normetanephrine) in combination with the immunological stress proxy leukocyte coping capacity (LCC) and rectal temperature. Additionally, we assessed the interconnections among different stress indices and their suitability as stress indicators to evaluate handling-induced stress in reindeer, where rectal temperature, other than serum cortisol levels, emerged as a robust and integrative stress parameter. Results Herding, handling, and restraint elicited a marked and seasonally different short-term stress response with higher stress mediator levels in winter. Further, females who had their calf removed shortly after parturition showed increased stress levels based on LCC. The repeated exposure to the same stressors led to a habituation, with decreasing levels of stress indices to the procedure in both seasons. This outcome implies that reindeer females in the present study were able to cope well with repeated manipulations and that this intensification may not compromise animal welfare. Notably, the traditional stress index body temperature correlated with various stress indices encompassing the HPA axis response (cortisol and corticosterone in summer and additionally cortisone and 11-deoxycortisol in winter), the sympathetic-adrenal-medullary system (metanephrine) as well as the immunological response to stress (LCC), in both seasons. Conclusion Our results emphasise body (rectal) temperature as a robust and integrative stress parameter in the context of our study. Our findings add to a foundation for evaluating available stress indices in different individual and environmental contexts and may contribute to improved animal management practices aimed at reducing stress levels and enhancing animal welfare.https://doi.org/10.1186/s12917-025-04718-8Context dependent stress responseGlucocorticoidsCatecholaminesLeukocyte coping capacityHabituation
spellingShingle Sebastian G. Vetter-Lang
Nikolaus Huber
Leif Egil Loe
Alina L. Evans
Jouko Kumpula
Per Medbøe Thorsby
Erik Ropstad
L. Monica Trondrud
Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
BMC Veterinary Research
Context dependent stress response
Glucocorticoids
Catecholamines
Leukocyte coping capacity
Habituation
title Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
title_full Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
title_fullStr Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
title_full_unstemmed Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
title_short Getting used to it? Stress of repeated management procedures in semi-domesticated reindeer
title_sort getting used to it stress of repeated management procedures in semi domesticated reindeer
topic Context dependent stress response
Glucocorticoids
Catecholamines
Leukocyte coping capacity
Habituation
url https://doi.org/10.1186/s12917-025-04718-8
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