Single-Component Starch-Based Hydrogels for Therapeutic Delivery
Hydrogels are interesting materials as delivery systems of various therapeutic agents, mainly due to the water-swollen network and the localized and sustained drug release. Herein, single-component starch-based hydrogels with enhanced degradation rates were produced by applying a facile synthesis an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/22/5463 |
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| author | Alfio Pulvirenti Antonella Caterina Boccia Carolina Constantin Mihaela Surcel Adriana Munteanu Victor-Eduard Peteu Monica Neagu |
| author_facet | Alfio Pulvirenti Antonella Caterina Boccia Carolina Constantin Mihaela Surcel Adriana Munteanu Victor-Eduard Peteu Monica Neagu |
| author_sort | Alfio Pulvirenti |
| collection | DOAJ |
| description | Hydrogels are interesting materials as delivery systems of various therapeutic agents, mainly due to the water-swollen network and the localized and sustained drug release. Herein, single-component starch-based hydrogels with enhanced degradation rates were produced by applying a facile synthesis and proposed for a novel delivery system of therapeutic molecules. Starch was oxidized with sodium periodate in water and mild conditions to generate aldehyde derivatives that, after a freeze-thaw procedure, were allowed to compact and stable hydrogels. Oxidized starch was also cross-linked with asparagine through a Schiff base reaction to link the active molecule directly to the polysaccharide structure. The materials were structurally and morphologically characterized, and the ability to adsorb and release over time an active molecule was proven by qNMR spectroscopy. The cytotoxicity was evaluated on CAL-27 cell line (oral squamous cell carcinoma). Results indicated that synthesized hydrogels lead to a “frozen proliferative” state on cells due to the swelling capability in the cell medium. This behavior was confirmed by flow cytometry data indicating the hydrogels induced less “early apoptosis” and more “late apoptosis” in the cells, compared to the untreated control. Since the proposed materials are able to control the cell proliferation, they could open a new scenario within the field of precise therapeutic applications. |
| format | Article |
| id | doaj-art-a90ea74ec12e444b8cd975d2b85c2297 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-a90ea74ec12e444b8cd975d2b85c22972025-08-20T02:48:01ZengMDPI AGMolecules1420-30492024-11-012922546310.3390/molecules29225463Single-Component Starch-Based Hydrogels for Therapeutic DeliveryAlfio Pulvirenti0Antonella Caterina Boccia1Carolina Constantin2Mihaela Surcel3Adriana Munteanu4Victor-Eduard Peteu5Monica Neagu6Istituto di Scienze e Tecnologie Chimiche (SCITEC) “Giulio Natta”, C.N.R., Via Alfonso Corti 12, 20133 Milano, ItalyIstituto di Scienze e Tecnologie Chimiche (SCITEC) “Giulio Natta”, C.N.R., Via Alfonso Corti 12, 20133 Milano, Italy“Victor Babes” National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania“Victor Babes” National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania“Victor Babes” National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania“Victor Babes” National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania“Victor Babes” National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, RomaniaHydrogels are interesting materials as delivery systems of various therapeutic agents, mainly due to the water-swollen network and the localized and sustained drug release. Herein, single-component starch-based hydrogels with enhanced degradation rates were produced by applying a facile synthesis and proposed for a novel delivery system of therapeutic molecules. Starch was oxidized with sodium periodate in water and mild conditions to generate aldehyde derivatives that, after a freeze-thaw procedure, were allowed to compact and stable hydrogels. Oxidized starch was also cross-linked with asparagine through a Schiff base reaction to link the active molecule directly to the polysaccharide structure. The materials were structurally and morphologically characterized, and the ability to adsorb and release over time an active molecule was proven by qNMR spectroscopy. The cytotoxicity was evaluated on CAL-27 cell line (oral squamous cell carcinoma). Results indicated that synthesized hydrogels lead to a “frozen proliferative” state on cells due to the swelling capability in the cell medium. This behavior was confirmed by flow cytometry data indicating the hydrogels induced less “early apoptosis” and more “late apoptosis” in the cells, compared to the untreated control. Since the proposed materials are able to control the cell proliferation, they could open a new scenario within the field of precise therapeutic applications.https://www.mdpi.com/1420-3049/29/22/5463hydrogelsstarchNMRflow cytometrycytotoxicitytherapeutic delivery |
| spellingShingle | Alfio Pulvirenti Antonella Caterina Boccia Carolina Constantin Mihaela Surcel Adriana Munteanu Victor-Eduard Peteu Monica Neagu Single-Component Starch-Based Hydrogels for Therapeutic Delivery Molecules hydrogels starch NMR flow cytometry cytotoxicity therapeutic delivery |
| title | Single-Component Starch-Based Hydrogels for Therapeutic Delivery |
| title_full | Single-Component Starch-Based Hydrogels for Therapeutic Delivery |
| title_fullStr | Single-Component Starch-Based Hydrogels for Therapeutic Delivery |
| title_full_unstemmed | Single-Component Starch-Based Hydrogels for Therapeutic Delivery |
| title_short | Single-Component Starch-Based Hydrogels for Therapeutic Delivery |
| title_sort | single component starch based hydrogels for therapeutic delivery |
| topic | hydrogels starch NMR flow cytometry cytotoxicity therapeutic delivery |
| url | https://www.mdpi.com/1420-3049/29/22/5463 |
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