Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau

Soil fungi serve as key mediators of belowground ecological processes; however, the altitudinal distribution patterns and their driving mechanisms of soil fungal communities in alpine shrubland ecosystems remain poorly understood. In this study, soil samples were collected from <i>Potentilla f...

Full description

Saved in:
Bibliographic Details
Main Authors: Lele Xie, Yushou Ma, Yanlong Wang, Yuan Ma, Yu Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/15/6/1345
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849423965784637440
author Lele Xie
Yushou Ma
Yanlong Wang
Yuan Ma
Yu Liu
author_facet Lele Xie
Yushou Ma
Yanlong Wang
Yuan Ma
Yu Liu
author_sort Lele Xie
collection DOAJ
description Soil fungi serve as key mediators of belowground ecological processes; however, the altitudinal distribution patterns and their driving mechanisms of soil fungal communities in alpine shrubland ecosystems remain poorly understood. In this study, soil samples were collected from <i>Potentilla fruticosa</i> shrubs at different altitudes, and their physical and chemical properties were determined. Illumina MiSeq sequencing technology was used to study the characteristics of soil fungal communities at different altitudes (3400, 3700, 4000, and 4300 m), and the driving factors affecting the composition of soil fungal communities were found through variance analysis and redundancy analysis. With the increase in altitude, species diversity decreased while total phosphorus and available phosphorus increased. Compared with 3400 m, the diversity index (Sobs, Chao1, and ACE index) of the soil fungal community at 4000 m is the highest, and that at 4300 m is the lowest. NMDS analysis showed that there were significant differences among soil fungal community structures at different altitudes. Redundancy analysis (RDA) indicated that available potassium, available phosphorus, and the Shannon–Wiener diversity index were the primary factors influencing the variation in soil fungal communities along the elevation gradient. Furthermore, the impact of soil physical and chemical properties on soil fungal communities was found to be more pronounced than that of plant characteristics. Network analysis shows that the network complexity is the highest at 4300 m above sea level. These studies provide a new perspective and basis for understanding the distribution pattern of soil fungi in the rhizosphere <i>Potentilla fruticosa</i> in the eastern Qinghai–Tibet Plateau.
format Article
id doaj-art-a90c0afcf3cd460dadbfc33e337abac8
institution Kabale University
issn 2073-4395
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Agronomy
spelling doaj-art-a90c0afcf3cd460dadbfc33e337abac82025-08-20T03:30:24ZengMDPI AGAgronomy2073-43952025-05-01156134510.3390/agronomy15061345Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet PlateauLele Xie0Yushou Ma1Yanlong Wang2Yuan Ma3Yu Liu4Academy of Animal and Veterinary Science, Qinghai University, Xining 810016, ChinaAcademy of Animal and Veterinary Science, Qinghai University, Xining 810016, ChinaAcademy of Animal and Veterinary Science, Qinghai University, Xining 810016, ChinaAcademy of Animal and Veterinary Science, Qinghai University, Xining 810016, ChinaShaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection, School of Ecology and Environment, Northwestern Polytechnical University, Xi’an 710129, ChinaSoil fungi serve as key mediators of belowground ecological processes; however, the altitudinal distribution patterns and their driving mechanisms of soil fungal communities in alpine shrubland ecosystems remain poorly understood. In this study, soil samples were collected from <i>Potentilla fruticosa</i> shrubs at different altitudes, and their physical and chemical properties were determined. Illumina MiSeq sequencing technology was used to study the characteristics of soil fungal communities at different altitudes (3400, 3700, 4000, and 4300 m), and the driving factors affecting the composition of soil fungal communities were found through variance analysis and redundancy analysis. With the increase in altitude, species diversity decreased while total phosphorus and available phosphorus increased. Compared with 3400 m, the diversity index (Sobs, Chao1, and ACE index) of the soil fungal community at 4000 m is the highest, and that at 4300 m is the lowest. NMDS analysis showed that there were significant differences among soil fungal community structures at different altitudes. Redundancy analysis (RDA) indicated that available potassium, available phosphorus, and the Shannon–Wiener diversity index were the primary factors influencing the variation in soil fungal communities along the elevation gradient. Furthermore, the impact of soil physical and chemical properties on soil fungal communities was found to be more pronounced than that of plant characteristics. Network analysis shows that the network complexity is the highest at 4300 m above sea level. These studies provide a new perspective and basis for understanding the distribution pattern of soil fungi in the rhizosphere <i>Potentilla fruticosa</i> in the eastern Qinghai–Tibet Plateau.https://www.mdpi.com/2073-4395/15/6/1345microorganismaltitude gradientsoil factoralpine shrubs
spellingShingle Lele Xie
Yushou Ma
Yanlong Wang
Yuan Ma
Yu Liu
Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
Agronomy
microorganism
altitude gradient
soil factor
alpine shrubs
title Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
title_full Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
title_fullStr Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
title_full_unstemmed Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
title_short Altitudinal Variation in Soil Fungal Community Associated with Alpine <i>Potentilla fruticosa</i> Shrublands in the Eastern Qinghai–Tibet Plateau
title_sort altitudinal variation in soil fungal community associated with alpine i potentilla fruticosa i shrublands in the eastern qinghai tibet plateau
topic microorganism
altitude gradient
soil factor
alpine shrubs
url https://www.mdpi.com/2073-4395/15/6/1345
work_keys_str_mv AT lelexie altitudinalvariationinsoilfungalcommunityassociatedwithalpineipotentillafruticosaishrublandsintheeasternqinghaitibetplateau
AT yushouma altitudinalvariationinsoilfungalcommunityassociatedwithalpineipotentillafruticosaishrublandsintheeasternqinghaitibetplateau
AT yanlongwang altitudinalvariationinsoilfungalcommunityassociatedwithalpineipotentillafruticosaishrublandsintheeasternqinghaitibetplateau
AT yuanma altitudinalvariationinsoilfungalcommunityassociatedwithalpineipotentillafruticosaishrublandsintheeasternqinghaitibetplateau
AT yuliu altitudinalvariationinsoilfungalcommunityassociatedwithalpineipotentillafruticosaishrublandsintheeasternqinghaitibetplateau