New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma
Abstract Medulloblastoma (MB), the most prevalent brain malignancy in children, presents significant challenges in paediatric oncology due to its aggressiveness and potential for relapse. Tailored treatments are crucial to mitigate treatment-related toxicities and long-term side effects on developin...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-05517-9 |
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| author | Denis Mustafov Laura Thomas Shoib S. Siddiqui George I. Lambrou Maria Braoudaki |
| author_facet | Denis Mustafov Laura Thomas Shoib S. Siddiqui George I. Lambrou Maria Braoudaki |
| author_sort | Denis Mustafov |
| collection | DOAJ |
| description | Abstract Medulloblastoma (MB), the most prevalent brain malignancy in children, presents significant challenges in paediatric oncology due to its aggressiveness and potential for relapse. Tailored treatments are crucial to mitigate treatment-related toxicities and long-term side effects on developing brains. Our study aimed to identify therapeutic targets for paediatric MB and explore common miRNA biosignatures with glioblastoma (GB), the most aggressive adult brain tumour. High-throughput small-RNA sequencing identified miR-206 and miR-383 as highly downregulated in MB samples, suggesting their tumour suppressor properties. Bioinformatics analysis identified CORO1C and SV2B as their targets. RT-qPCR, western blotting, and immunohistochemistry confirmed their overexpression in MB and GB. Elevated CORO1C expression was also found in adult MB and GB tissue samples. The role of both miRNAs on their target genes was validated through in vitro functional assays. Our study uncovers the potential role of miR-206/CORO1C and miR-383/SV2B axes as innovative therapeutic targets for combating aggressive paediatric and adult brain malignancies. |
| format | Article |
| id | doaj-art-a8f2dafff1dd4a78943b73eaccce8734 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a8f2dafff1dd4a78943b73eaccce87342025-08-20T04:01:34ZengNature PortfolioScientific Reports2045-23222025-07-0115111810.1038/s41598-025-05517-9New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastomaDenis Mustafov0Laura Thomas1Shoib S. Siddiqui2George I. Lambrou3Maria Braoudaki4School of Health, Medicine and Life Sciences, University of HertfordshireSchool of Health, Medicine and Life Sciences, University of HertfordshireSchool of Health, Medicine and Life Sciences, University of HertfordshireChoremeio Research Laboratory, First Department of Pediatrics, School of Medicine, National and Kapodistrian University of AthensSchool of Health, Medicine and Life Sciences, University of HertfordshireAbstract Medulloblastoma (MB), the most prevalent brain malignancy in children, presents significant challenges in paediatric oncology due to its aggressiveness and potential for relapse. Tailored treatments are crucial to mitigate treatment-related toxicities and long-term side effects on developing brains. Our study aimed to identify therapeutic targets for paediatric MB and explore common miRNA biosignatures with glioblastoma (GB), the most aggressive adult brain tumour. High-throughput small-RNA sequencing identified miR-206 and miR-383 as highly downregulated in MB samples, suggesting their tumour suppressor properties. Bioinformatics analysis identified CORO1C and SV2B as their targets. RT-qPCR, western blotting, and immunohistochemistry confirmed their overexpression in MB and GB. Elevated CORO1C expression was also found in adult MB and GB tissue samples. The role of both miRNAs on their target genes was validated through in vitro functional assays. Our study uncovers the potential role of miR-206/CORO1C and miR-383/SV2B axes as innovative therapeutic targets for combating aggressive paediatric and adult brain malignancies.https://doi.org/10.1038/s41598-025-05517-9 |
| spellingShingle | Denis Mustafov Laura Thomas Shoib S. Siddiqui George I. Lambrou Maria Braoudaki New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma Scientific Reports |
| title | New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| title_full | New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| title_fullStr | New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| title_full_unstemmed | New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| title_short | New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| title_sort | new microrna based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma |
| url | https://doi.org/10.1038/s41598-025-05517-9 |
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