Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance

The present study aims to evaluate the effects of feeding dietary garden cress seeds (Lepidium sativum) (GC) at doses of 10, 20, and 40 g/kg diet for 77 days to seabream (Sparus aurata) fingerlings (starting weight = 0.84 ± 0.04 g/fish). Hematological indicators, body composition, feed consumption,...

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Main Authors: Mohamed M. Toutou, Mohamed M. Hebalah, Manal I. El-Barbary, Ehab El-Haroun, Norhan E. Saleh
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S235251342500273X
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author Mohamed M. Toutou
Mohamed M. Hebalah
Manal I. El-Barbary
Ehab El-Haroun
Norhan E. Saleh
author_facet Mohamed M. Toutou
Mohamed M. Hebalah
Manal I. El-Barbary
Ehab El-Haroun
Norhan E. Saleh
author_sort Mohamed M. Toutou
collection DOAJ
description The present study aims to evaluate the effects of feeding dietary garden cress seeds (Lepidium sativum) (GC) at doses of 10, 20, and 40 g/kg diet for 77 days to seabream (Sparus aurata) fingerlings (starting weight = 0.84 ± 0.04 g/fish). Hematological indicators, body composition, feed consumption, growth performance, liver biomarkers, and exposure to heat stress are the assessed parameters. The current findings indicated that seabream fingerlings growth and feed consumption might be enhanced by the dietary GC administration at the level of 40 g/kg diet. The findings showed that GC had a beneficial impact on the fish protein and fat content. Additionally, the findings demonstrated that GC considerably enhanced the hematological parameters (hematocrit, hemoglobin, and the number of white and red blood cells). Serum biomarkers’ values indicated an enhancement in the total protein, albumin, globulin, liver and kidney functions at 40 g/kg supplementation level. Furthermore, at this concentration, the glucose level was depressed meanwhile; the liver glycogen level was increased. Results showed that the use of GC at a level of 40 g/kg decreased the oxidative stress and improved the fish immunity depending on the values of SOD, MDA and lysozyme activities. Fish were subjected to a thermal stress at the end of the experiment at 4 and 32°C, and the results indicated that the fish treated with varying concentrations of GC, as a feed additives showed better resistance than the control group. The histo-pathological examination for three internal organs namely; liver, kidney, and intestine illustrated positive effects on the organs architecture when GC was supplemented in fish diets.
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spelling doaj-art-a8f04328224b46868fff362da3d168dd2025-08-20T03:45:10ZengElsevierAquaculture Reports2352-51342025-09-014310288710.1016/j.aqrep.2025.102887Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress toleranceMohamed M. Toutou0Mohamed M. Hebalah1Manal I. El-Barbary2Ehab El-Haroun3Norhan E. Saleh4National Institute of Oceanography and Fisheries, NIOF, EgyptNational Institute of Oceanography and Fisheries, NIOF, EgyptNational Institute of Oceanography and Fisheries, NIOF, EgyptDepartment of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates University, P.O. Box 15551, Al Ain, Abu Dhabi, United Arab Emirates; Corresponding authors.National Institute of Oceanography and Fisheries, NIOF, Egypt; Corresponding authors.The present study aims to evaluate the effects of feeding dietary garden cress seeds (Lepidium sativum) (GC) at doses of 10, 20, and 40 g/kg diet for 77 days to seabream (Sparus aurata) fingerlings (starting weight = 0.84 ± 0.04 g/fish). Hematological indicators, body composition, feed consumption, growth performance, liver biomarkers, and exposure to heat stress are the assessed parameters. The current findings indicated that seabream fingerlings growth and feed consumption might be enhanced by the dietary GC administration at the level of 40 g/kg diet. The findings showed that GC had a beneficial impact on the fish protein and fat content. Additionally, the findings demonstrated that GC considerably enhanced the hematological parameters (hematocrit, hemoglobin, and the number of white and red blood cells). Serum biomarkers’ values indicated an enhancement in the total protein, albumin, globulin, liver and kidney functions at 40 g/kg supplementation level. Furthermore, at this concentration, the glucose level was depressed meanwhile; the liver glycogen level was increased. Results showed that the use of GC at a level of 40 g/kg decreased the oxidative stress and improved the fish immunity depending on the values of SOD, MDA and lysozyme activities. Fish were subjected to a thermal stress at the end of the experiment at 4 and 32°C, and the results indicated that the fish treated with varying concentrations of GC, as a feed additives showed better resistance than the control group. The histo-pathological examination for three internal organs namely; liver, kidney, and intestine illustrated positive effects on the organs architecture when GC was supplemented in fish diets.http://www.sciencedirect.com/science/article/pii/S235251342500273XSerum biomarkersLiver and kidney functionsHematological analysisOxidative stressImmunityThermal stress test
spellingShingle Mohamed M. Toutou
Mohamed M. Hebalah
Manal I. El-Barbary
Ehab El-Haroun
Norhan E. Saleh
Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
Aquaculture Reports
Serum biomarkers
Liver and kidney functions
Hematological analysis
Oxidative stress
Immunity
Thermal stress test
title Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
title_full Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
title_fullStr Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
title_full_unstemmed Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
title_short Assessment of garden cress (Lepidium sativum) seeds supplementation on Gilthead seabream (Sparus aurata) growth, general health, immunity, histopathology, and heat stress tolerance
title_sort assessment of garden cress lepidium sativum seeds supplementation on gilthead seabream sparus aurata growth general health immunity histopathology and heat stress tolerance
topic Serum biomarkers
Liver and kidney functions
Hematological analysis
Oxidative stress
Immunity
Thermal stress test
url http://www.sciencedirect.com/science/article/pii/S235251342500273X
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