Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities
Abstract Tumor organoid models have emerged as valuable 3D in vitro systems to study cancer behavior in a physiologically relevant environment. The composition and architecture of the extracellular matrix (ECM) play critical roles in tumor organoid culture by influencing the tumor microenvironment a...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-025-06349-x |
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| author | Kan Li Yibo He Xue Jin Ketao Jin Jun Qian |
| author_facet | Kan Li Yibo He Xue Jin Ketao Jin Jun Qian |
| author_sort | Kan Li |
| collection | DOAJ |
| description | Abstract Tumor organoid models have emerged as valuable 3D in vitro systems to study cancer behavior in a physiologically relevant environment. The composition and architecture of the extracellular matrix (ECM) play critical roles in tumor organoid culture by influencing the tumor microenvironment and tumor behavior. Traditional matrices such as Matrigel and collagen, have been widely used, but their batch-to-batch variability and limited tunability hinder their reproducibility and broader applications. To address these challenges, researchers have turned to synthetic/engineered matrices and biopolymer-based matrices, which offer precise tunability, reproducibility, and chemically defined compositions. However, these matrices also present challenges of their own. In this review, we explore the significance of ECMs in tumor organoid culture, discuss the limitations of commonly used matrices, and highlight recent advancements in engineered/synthetic matrices for improved tumor organoid modeling. |
| format | Article |
| id | doaj-art-a8e8b9c39b484e989ac9b00c53002acf |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-a8e8b9c39b484e989ac9b00c53002acf2025-08-20T02:55:32ZengBMCJournal of Translational Medicine1479-58762025-05-0123111610.1186/s12967-025-06349-xReproducible extracellular matrices for tumor organoid culture: challenges and opportunitiesKan Li0Yibo He1Xue Jin2Ketao Jin3Jun Qian4School of Basic Medical Sciences, Health Science Center, Ningbo UniversityDepartment of Surgical Oncology, Hangzhou Cancer Hospital, HangzhouCenter for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People’S Hospital (Affiliated People’S Hospital, Hangzhou Medical College)Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)Department of Colorectal Surgery, Xinchang People’S Hospital, Affiliated Xinchang Hosptial, Wenzhou Medical UniversityAbstract Tumor organoid models have emerged as valuable 3D in vitro systems to study cancer behavior in a physiologically relevant environment. The composition and architecture of the extracellular matrix (ECM) play critical roles in tumor organoid culture by influencing the tumor microenvironment and tumor behavior. Traditional matrices such as Matrigel and collagen, have been widely used, but their batch-to-batch variability and limited tunability hinder their reproducibility and broader applications. To address these challenges, researchers have turned to synthetic/engineered matrices and biopolymer-based matrices, which offer precise tunability, reproducibility, and chemically defined compositions. However, these matrices also present challenges of their own. In this review, we explore the significance of ECMs in tumor organoid culture, discuss the limitations of commonly used matrices, and highlight recent advancements in engineered/synthetic matrices for improved tumor organoid modeling.https://doi.org/10.1186/s12967-025-06349-xExtracellular matrixReproducibilityTumor microenvironmentTumor organoidTunability |
| spellingShingle | Kan Li Yibo He Xue Jin Ketao Jin Jun Qian Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities Journal of Translational Medicine Extracellular matrix Reproducibility Tumor microenvironment Tumor organoid Tunability |
| title | Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities |
| title_full | Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities |
| title_fullStr | Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities |
| title_full_unstemmed | Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities |
| title_short | Reproducible extracellular matrices for tumor organoid culture: challenges and opportunities |
| title_sort | reproducible extracellular matrices for tumor organoid culture challenges and opportunities |
| topic | Extracellular matrix Reproducibility Tumor microenvironment Tumor organoid Tunability |
| url | https://doi.org/10.1186/s12967-025-06349-x |
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