Growth Pattern and Copper Accumulation in Callus of Datura metel
<p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper comp...
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Universitas Negeri Semarang
2016-09-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5177 |
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| author | Yulita Nurchayati Santosa Santosa Laurentius Hartanto Nugroho Ari Indrianto |
| author_facet | Yulita Nurchayati Santosa Santosa Laurentius Hartanto Nugroho Ari Indrianto |
| author_sort | Yulita Nurchayati |
| collection | DOAJ |
| description | <p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl<sub>2</sub>.2H<sub>2</sub>O and Na<sub>2</sub>CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 µM. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20<sup>th</sup> doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl<sub>2</sub>.2H<sub>2</sub>O at 5 – 15 µM of level concentration but declined at 20µM. The maximum rate of accumulation of Cu (0,1519 mg g<sup>-1</sup>) was obtained at 15 µM. Instead, the addition of Na<sub>2</sub>CuEDTA at 5 – 20µM of level concentration showed the significant increment while the maximum accumulation was obtained at 20µM (0,1420 mg g<sup>-1</sup>). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of <em>D. metel</em> as that fairly absorbed and accumulated Cu<sup>2+</sup>. Exposure with CuCl<sub>2</sub>.2H<sub>2</sub>O indicated higher accumulation than Na<sub>2</sub>CuEDTA.</p><p><strong>How to Cite</strong></p><p>Nurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(2), 135-140. </p> |
| format | Article |
| id | doaj-art-a8e7254d0c3a46ceaa4bfdf7b85eb548 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Universitas Negeri Semarang |
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| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-a8e7254d0c3a46ceaa4bfdf7b85eb5482025-08-20T03:18:49ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102016-09-018213514010.15294/biosaintifika.v8i2.51774551Growth Pattern and Copper Accumulation in Callus of Datura metelYulita Nurchayati0Santosa Santosa1Laurentius Hartanto Nugroho2Ari Indrianto3Postgraduate Program, Faculty of Biology, Universitas Gadjah Mada, IndonesiaPlant Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, IndonesiaPlant Structure and Development Laboratory, Faculty of Biology, Universitas Gadjah Mada, IndonesiaBiotechnology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia<p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl<sub>2</sub>.2H<sub>2</sub>O and Na<sub>2</sub>CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 µM. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20<sup>th</sup> doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl<sub>2</sub>.2H<sub>2</sub>O at 5 – 15 µM of level concentration but declined at 20µM. The maximum rate of accumulation of Cu (0,1519 mg g<sup>-1</sup>) was obtained at 15 µM. Instead, the addition of Na<sub>2</sub>CuEDTA at 5 – 20µM of level concentration showed the significant increment while the maximum accumulation was obtained at 20µM (0,1420 mg g<sup>-1</sup>). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of <em>D. metel</em> as that fairly absorbed and accumulated Cu<sup>2+</sup>. Exposure with CuCl<sub>2</sub>.2H<sub>2</sub>O indicated higher accumulation than Na<sub>2</sub>CuEDTA.</p><p><strong>How to Cite</strong></p><p>Nurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(2), 135-140. </p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5177Datura metel L. callus cultureCopperBioaccumulationPhytoremediation |
| spellingShingle | Yulita Nurchayati Santosa Santosa Laurentius Hartanto Nugroho Ari Indrianto Growth Pattern and Copper Accumulation in Callus of Datura metel Biosaintifika: Journal of Biology & Biology Education Datura metel L. callus culture Copper Bioaccumulation Phytoremediation |
| title | Growth Pattern and Copper Accumulation in Callus of Datura metel |
| title_full | Growth Pattern and Copper Accumulation in Callus of Datura metel |
| title_fullStr | Growth Pattern and Copper Accumulation in Callus of Datura metel |
| title_full_unstemmed | Growth Pattern and Copper Accumulation in Callus of Datura metel |
| title_short | Growth Pattern and Copper Accumulation in Callus of Datura metel |
| title_sort | growth pattern and copper accumulation in callus of datura metel |
| topic | Datura metel L. callus culture Copper Bioaccumulation Phytoremediation |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5177 |
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