Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i>
Plant biotechnology creates opportunities for the cultivation of plants regardless of their natural habitats, which are often protected or difficult to access. Maintaining suspension cell cultures in bioreactors is an advanced part of biotechnological research that provides possibilities for obtaini...
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2024-11-01
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| author | Karolina Jafernik Adam Kokotkiewicz Michał Dziurka Aleksandra Kruk Anna Hering Krzysztof Jędrzejewski Piotr Waligórski Piotr Graczyk Paweł Kubica Justyna Stefanowicz-Hajduk Sebastian Granica Maria Łuczkiewicz Agnieszka Szopa |
| author_facet | Karolina Jafernik Adam Kokotkiewicz Michał Dziurka Aleksandra Kruk Anna Hering Krzysztof Jędrzejewski Piotr Waligórski Piotr Graczyk Paweł Kubica Justyna Stefanowicz-Hajduk Sebastian Granica Maria Łuczkiewicz Agnieszka Szopa |
| author_sort | Karolina Jafernik |
| collection | DOAJ |
| description | Plant biotechnology creates opportunities for the cultivation of plants regardless of their natural habitats, which are often protected or difficult to access. Maintaining suspension cell cultures in bioreactors is an advanced part of biotechnological research that provides possibilities for obtaining plant tissue on a large scale. In this study, the suspension culture cultivation of a Chinese endemic plant, <i>Schisandra henryi</i>, in a stirred tank bioreactor was elaborated for the first time. The phytochemical profile of the tissue extracts was determined with UHPLC-MS/MS for the lignans (fifteen dibenzocyclooctadiene lignans, one aryltetralin lignan, and two neolignans) and UHPLC-DAD-ESI-MS<sup>3</sup> for the phenolic compounds (procyanidins and their derivatives and catechin). The maximum total lignan content of 1289 µg/100 g DW was detected for the extracts from suspensions cultured in a bioreactor for over 10 days. For the phenolic compounds, catechin was the dominant compound (390.44 mg/100 g DW). The biological activity of the extracts was tested too. To determine antioxidant potential we used DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Molybdenum reduction, and β-carotene bleaching tests. The inhibition activity of the <i>S. henryi</i> extract on the enzymes responsible for skin aging, hyaluronidase and tyrosinase, was assessed with spectrophotometry. The cytotoxic activity of the extracts was estimated on human ovarian SKOV-3, cervical HeLa, and gastric AGS cancer cells and non-cancer, normal fibroblasts by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results showed the great potential of the obtained cell biomass extracts. The results of the antioxidant tests indicated their strong ability to reduce the level of free radicals, similarly to that of ascorbic acid, as well as the weak capacity to protect lipids from oxidation. Moreover, anticancer potential, particularly on the cervical and gastric cancer cells, was confirmed too. |
| format | Article |
| id | doaj-art-a8d8c08b77fa42bb88527da3e02104b7 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-a8d8c08b77fa42bb88527da3e02104b72025-08-20T02:48:06ZengMDPI AGMolecules1420-30492024-11-012922526010.3390/molecules29225260Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i>Karolina Jafernik0Adam Kokotkiewicz1Michał Dziurka2Aleksandra Kruk3Anna Hering4Krzysztof Jędrzejewski5Piotr Waligórski6Piotr Graczyk7Paweł Kubica8Justyna Stefanowicz-Hajduk9Sebastian Granica10Maria Łuczkiewicz11Agnieszka Szopa12Department of Medicinal Plant and Mushroom Biotechnology, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, PolandDepartment of Pharmacognosy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandPolish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, ul. Niezapominajek 21, 30-239 Kraków, PolandMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, ul. Banacha 1, 02-097 Warszawa, PolandDepartment of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandDepartment of Pharmacognosy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandPolish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, ul. Niezapominajek 21, 30-239 Kraków, PolandDepartment of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, PolandDepartment of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of Warsaw, ul. Banacha 1, 02-097 Warszawa, PolandDepartment of Pharmacognosy, Medical University of Gdańsk, Al. Generała Józefa Hallera 107, 80-416 Gdańsk, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, PolandPlant biotechnology creates opportunities for the cultivation of plants regardless of their natural habitats, which are often protected or difficult to access. Maintaining suspension cell cultures in bioreactors is an advanced part of biotechnological research that provides possibilities for obtaining plant tissue on a large scale. In this study, the suspension culture cultivation of a Chinese endemic plant, <i>Schisandra henryi</i>, in a stirred tank bioreactor was elaborated for the first time. The phytochemical profile of the tissue extracts was determined with UHPLC-MS/MS for the lignans (fifteen dibenzocyclooctadiene lignans, one aryltetralin lignan, and two neolignans) and UHPLC-DAD-ESI-MS<sup>3</sup> for the phenolic compounds (procyanidins and their derivatives and catechin). The maximum total lignan content of 1289 µg/100 g DW was detected for the extracts from suspensions cultured in a bioreactor for over 10 days. For the phenolic compounds, catechin was the dominant compound (390.44 mg/100 g DW). The biological activity of the extracts was tested too. To determine antioxidant potential we used DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Molybdenum reduction, and β-carotene bleaching tests. The inhibition activity of the <i>S. henryi</i> extract on the enzymes responsible for skin aging, hyaluronidase and tyrosinase, was assessed with spectrophotometry. The cytotoxic activity of the extracts was estimated on human ovarian SKOV-3, cervical HeLa, and gastric AGS cancer cells and non-cancer, normal fibroblasts by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results showed the great potential of the obtained cell biomass extracts. The results of the antioxidant tests indicated their strong ability to reduce the level of free radicals, similarly to that of ascorbic acid, as well as the weak capacity to protect lipids from oxidation. Moreover, anticancer potential, particularly on the cervical and gastric cancer cells, was confirmed too.https://www.mdpi.com/1420-3049/29/22/5260<i>Schisandra henryi</i>plant culturephenolic compoundsdibenzocyclooctadiene lignansantioxidant potentialcytotoxic activity |
| spellingShingle | Karolina Jafernik Adam Kokotkiewicz Michał Dziurka Aleksandra Kruk Anna Hering Krzysztof Jędrzejewski Piotr Waligórski Piotr Graczyk Paweł Kubica Justyna Stefanowicz-Hajduk Sebastian Granica Maria Łuczkiewicz Agnieszka Szopa Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> Molecules <i>Schisandra henryi</i> plant culture phenolic compounds dibenzocyclooctadiene lignans antioxidant potential cytotoxic activity |
| title | Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> |
| title_full | Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> |
| title_fullStr | Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> |
| title_full_unstemmed | Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> |
| title_short | Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of <i>Schisandra henryi</i> |
| title_sort | phytochemical profiling and biological activities of extracts from bioreactor grown suspension cell cultures of i schisandra henryi i |
| topic | <i>Schisandra henryi</i> plant culture phenolic compounds dibenzocyclooctadiene lignans antioxidant potential cytotoxic activity |
| url | https://www.mdpi.com/1420-3049/29/22/5260 |
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