Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine
Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concern...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2014/291458 |
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| author | Akhilesh Kumar Verma Subhash Chandra Rana Gopal Singh Tej Bali Singh Shalabh Srivastava Ragini Srivastava |
| author_facet | Akhilesh Kumar Verma Subhash Chandra Rana Gopal Singh Tej Bali Singh Shalabh Srivastava Ragini Srivastava |
| author_sort | Akhilesh Kumar Verma |
| collection | DOAJ |
| description | Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased (P<0.001). Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 (P<0.001). Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 (P<0.001). In case-2, serum creatinine was significantly correlated with SPA only (P<0.001). Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage. |
| format | Article |
| id | doaj-art-a8c3876a010041d4a991af168e8328d1 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-a8c3876a010041d4a991af168e8328d12025-02-03T05:58:25ZengWileyBiochemistry Research International2090-22472090-22552014-01-01201410.1155/2014/291458291458Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and CreatinineAkhilesh Kumar Verma0Subhash Chandra1Rana Gopal Singh2Tej Bali Singh3Shalabh Srivastava4Ragini Srivastava5Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDepartment of Nephrology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDepartment of Nephrology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDivision of Biostatistics, Department of Community Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDepartment of Oral Pathology, Jaipur Dental College, Jaipur, Rajasthan 302020, IndiaDepartment of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaAssociation of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased (P<0.001). Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 (P<0.001). Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 (P<0.001). In case-2, serum creatinine was significantly correlated with SPA only (P<0.001). Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage.http://dx.doi.org/10.1155/2014/291458 |
| spellingShingle | Akhilesh Kumar Verma Subhash Chandra Rana Gopal Singh Tej Bali Singh Shalabh Srivastava Ragini Srivastava Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine Biochemistry Research International |
| title | Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine |
| title_full | Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine |
| title_fullStr | Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine |
| title_full_unstemmed | Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine |
| title_short | Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine |
| title_sort | serum prolidase activity and oxidative stress in diabetic nephropathy and end stage renal disease a correlative study with glucose and creatinine |
| url | http://dx.doi.org/10.1155/2014/291458 |
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