In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters
Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of My...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2016/2839041 |
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| author | Mariangela Palmieri Palma Papale Antonietta Della Ragione Giuseppa Quaranta Giovanni Russo Sabatino Russo |
| author_facet | Mariangela Palmieri Palma Papale Antonietta Della Ragione Giuseppa Quaranta Giovanni Russo Sabatino Russo |
| author_sort | Mariangela Palmieri |
| collection | DOAJ |
| description | Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motility of spermatozoa, in order to better validate its possible practical application in vitro, in order to improve the capacitation protocols commonly used in Assisted Reproductive Technology (ART). A total of 100 fresh and 25 thawed semen samples were analyzed in vitro prior to and after addition of Myo-Ins. Treatment of samples with Myo-Ins showed an increase in the sperm total and progressive motility in both fresh and thawed samples. Furthermore, Myo-Ins proved to be well tolerated by spermatozoa in vitro, demonstrating that it can be efficiently and safely used as antioxidant in the laboratory practice and for preparation of semen samples in ART. |
| format | Article |
| id | doaj-art-a8c3387bc102477a9d41aa5ccebe6672 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-a8c3387bc102477a9d41aa5ccebe66722025-08-20T02:20:44ZengWileyInternational Journal of Endocrinology1687-83371687-83452016-01-01201610.1155/2016/28390412839041In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic ParametersMariangela Palmieri0Palma Papale1Antonietta Della Ragione2Giuseppa Quaranta3Giovanni Russo4Sabatino Russo5Assisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyAssisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyAssisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyAssisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyAssisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyAssisted Reproductive Technologies, Clinic Center Hera, Giugliano in Campania, ItalyMale infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motility of spermatozoa, in order to better validate its possible practical application in vitro, in order to improve the capacitation protocols commonly used in Assisted Reproductive Technology (ART). A total of 100 fresh and 25 thawed semen samples were analyzed in vitro prior to and after addition of Myo-Ins. Treatment of samples with Myo-Ins showed an increase in the sperm total and progressive motility in both fresh and thawed samples. Furthermore, Myo-Ins proved to be well tolerated by spermatozoa in vitro, demonstrating that it can be efficiently and safely used as antioxidant in the laboratory practice and for preparation of semen samples in ART.http://dx.doi.org/10.1155/2016/2839041 |
| spellingShingle | Mariangela Palmieri Palma Papale Antonietta Della Ragione Giuseppa Quaranta Giovanni Russo Sabatino Russo In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters International Journal of Endocrinology |
| title | In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters |
| title_full | In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters |
| title_fullStr | In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters |
| title_full_unstemmed | In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters |
| title_short | In Vitro Antioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters |
| title_sort | in vitro antioxidant treatment of semen samples in assisted reproductive technology effects of myo inositol on nemaspermic parameters |
| url | http://dx.doi.org/10.1155/2016/2839041 |
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