Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors
The influence of introducing fluctuations in acetate feeding concentrations on the process stability of a lab-scale horizontal anaerobic reactor (HAR) was investigated to ascertain its effects on acetoclastic methanogenesis. Acetate concentrations were randomized at 85 g COD/L ± 20% and discontinuou...
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| author | Jovale Vincent Tongco Md Abu Hanifa Jannat Sangmin Kim Sang Hyeok Park Seokhwan Hwang |
| author_facet | Jovale Vincent Tongco Md Abu Hanifa Jannat Sangmin Kim Sang Hyeok Park Seokhwan Hwang |
| author_sort | Jovale Vincent Tongco |
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| description | The influence of introducing fluctuations in acetate feeding concentrations on the process stability of a lab-scale horizontal anaerobic reactor (HAR) was investigated to ascertain its effects on acetoclastic methanogenesis. Acetate concentrations were randomized at 85 g COD/L ± 20% and discontinuously fed in the duplicate HARs for five days per week (giving the system time to rest and equilibrate for two days). The reactors were monitored daily with respect to performance indicators (physicochemical variables). The residual acetate concentration was observed to fluctuate at the initial stages, followed by a converging trend (decrease in variance) until the end of operation. Furthermore, letting the system self-neutralize and equilibrate during rest days resulted in improved process performance. The daily acetate degradation efficiency at ~90% and methane concentration at ~60% were attained after operating the reactors for 80 days. The results divulged that introducing fluctuations in acetate feeding concentrations does not affect the stability of biogas production and methane concentration. The acclimatization of the methanogenic population (predominantly <i>Methanosaeta,</i> then shifting to <i>Methanosarcina</i>) was also observed. |
| format | Article |
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| issn | 1996-1073 |
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| spelling | doaj-art-a8b56df3fb6243aa9e203d650855ca0a2025-08-20T02:24:43ZengMDPI AGEnergies1996-10732025-06-011812312010.3390/en18123120Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic ReactorsJovale Vincent Tongco0Md Abu Hanifa Jannat1Sangmin Kim2Sang Hyeok Park3Seokhwan Hwang4Department of Forest, Rangeland and Fire Sciences, University of Idaho, 875 Perimeter Dr, Moscow, ID 83844, USADivision of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of KoreaDivision of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of KoreaDivision of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of KoreaDivision of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of KoreaThe influence of introducing fluctuations in acetate feeding concentrations on the process stability of a lab-scale horizontal anaerobic reactor (HAR) was investigated to ascertain its effects on acetoclastic methanogenesis. Acetate concentrations were randomized at 85 g COD/L ± 20% and discontinuously fed in the duplicate HARs for five days per week (giving the system time to rest and equilibrate for two days). The reactors were monitored daily with respect to performance indicators (physicochemical variables). The residual acetate concentration was observed to fluctuate at the initial stages, followed by a converging trend (decrease in variance) until the end of operation. Furthermore, letting the system self-neutralize and equilibrate during rest days resulted in improved process performance. The daily acetate degradation efficiency at ~90% and methane concentration at ~60% were attained after operating the reactors for 80 days. The results divulged that introducing fluctuations in acetate feeding concentrations does not affect the stability of biogas production and methane concentration. The acclimatization of the methanogenic population (predominantly <i>Methanosaeta,</i> then shifting to <i>Methanosarcina</i>) was also observed.https://www.mdpi.com/1996-1073/18/12/3120anaerobic digestionhorizontal reactoracetoclastic methanogenesissubstrate concentration fluctuationmicrobial community analysis |
| spellingShingle | Jovale Vincent Tongco Md Abu Hanifa Jannat Sangmin Kim Sang Hyeok Park Seokhwan Hwang Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors Energies anaerobic digestion horizontal reactor acetoclastic methanogenesis substrate concentration fluctuation microbial community analysis |
| title | Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors |
| title_full | Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors |
| title_fullStr | Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors |
| title_full_unstemmed | Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors |
| title_short | Process Performance and Biogas Output: Impact of Fluctuating Acetate Concentrations on Methanogenesis in Horizontal Anaerobic Reactors |
| title_sort | process performance and biogas output impact of fluctuating acetate concentrations on methanogenesis in horizontal anaerobic reactors |
| topic | anaerobic digestion horizontal reactor acetoclastic methanogenesis substrate concentration fluctuation microbial community analysis |
| url | https://www.mdpi.com/1996-1073/18/12/3120 |
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