Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success
Variability of pancreatic donors may significantly impact the success of islet isolation. The aim of this study was to evaluate donor factors associated with isolation failure and to investigate whether immunohistology could contribute to organ selection. Donor characteristics were evaluated for bot...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4214328 |
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| author | Zuzana Berkova Frantisek Saudek Peter Girman Klara Zacharovova Jan Kriz Eva Fabryova Ivan Leontovyc Tomas Koblas Lucie Kosinova Tomas Neskudla Ema Vavrova David Habart Sarka Loukotova Martina Zahradnicka Kvetoslav Lipar Ludek Voska Jelena Skibova |
| author_facet | Zuzana Berkova Frantisek Saudek Peter Girman Klara Zacharovova Jan Kriz Eva Fabryova Ivan Leontovyc Tomas Koblas Lucie Kosinova Tomas Neskudla Ema Vavrova David Habart Sarka Loukotova Martina Zahradnicka Kvetoslav Lipar Ludek Voska Jelena Skibova |
| author_sort | Zuzana Berkova |
| collection | DOAJ |
| description | Variability of pancreatic donors may significantly impact the success of islet isolation. The aim of this study was to evaluate donor factors associated with isolation failure and to investigate whether immunohistology could contribute to organ selection. Donor characteristics were evaluated for both successful (n=61) and failed (n=98) islet isolations. Samples of donor pancreatic tissue (n=78) were taken for immunohistochemical examination. Islet isolations with 250000 islet equivalents were considered successful. We confirmed that BMI of less than 25 kg/m2 (P<0.001), cold ischemia time more than 8 hours (P<0.01), hospitalization longer than 96 hours (P<0.05), higher catecholamine doses (P<0.05), and edematous pancreases (P<0.01) all unfavorably affected isolation outcome. Subsequent immunohistochemical examination of donor pancreases confirmed significant differences in insulin-positive areas (P<0.001). ROC analyses then established that the insulin-positive area in the pancreas could be used to predict the likely success of islet isolation (P<0.001). At the optimal cutoff point (>1.02%), sensitivity and specificity were 89% and 76%, respectively. To conclude, while the insulin-positive area, determined preislet isolation, as a single variable, is sufficient to predict isolation outcome and helps to improve the success of this procedure, its combination with the established donor scoring system might further improve organ selection. |
| format | Article |
| id | doaj-art-a8a9785c23554c80bfd8aefe04b6a3aa |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-a8a9785c23554c80bfd8aefe04b6a3aa2025-08-20T02:20:45ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/42143284214328Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation SuccessZuzana Berkova0Frantisek Saudek1Peter Girman2Klara Zacharovova3Jan Kriz4Eva Fabryova5Ivan Leontovyc6Tomas Koblas7Lucie Kosinova8Tomas Neskudla9Ema Vavrova10David Habart11Sarka Loukotova12Martina Zahradnicka13Kvetoslav Lipar14Ludek Voska15Jelena Skibova16Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicLaboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Diabetes, Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicTransplant Surgery Department, Transplant Centre, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Clinical and Transplant Pathology, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Medical Statistics, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicVariability of pancreatic donors may significantly impact the success of islet isolation. The aim of this study was to evaluate donor factors associated with isolation failure and to investigate whether immunohistology could contribute to organ selection. Donor characteristics were evaluated for both successful (n=61) and failed (n=98) islet isolations. Samples of donor pancreatic tissue (n=78) were taken for immunohistochemical examination. Islet isolations with 250000 islet equivalents were considered successful. We confirmed that BMI of less than 25 kg/m2 (P<0.001), cold ischemia time more than 8 hours (P<0.01), hospitalization longer than 96 hours (P<0.05), higher catecholamine doses (P<0.05), and edematous pancreases (P<0.01) all unfavorably affected isolation outcome. Subsequent immunohistochemical examination of donor pancreases confirmed significant differences in insulin-positive areas (P<0.001). ROC analyses then established that the insulin-positive area in the pancreas could be used to predict the likely success of islet isolation (P<0.001). At the optimal cutoff point (>1.02%), sensitivity and specificity were 89% and 76%, respectively. To conclude, while the insulin-positive area, determined preislet isolation, as a single variable, is sufficient to predict isolation outcome and helps to improve the success of this procedure, its combination with the established donor scoring system might further improve organ selection.http://dx.doi.org/10.1155/2016/4214328 |
| spellingShingle | Zuzana Berkova Frantisek Saudek Peter Girman Klara Zacharovova Jan Kriz Eva Fabryova Ivan Leontovyc Tomas Koblas Lucie Kosinova Tomas Neskudla Ema Vavrova David Habart Sarka Loukotova Martina Zahradnicka Kvetoslav Lipar Ludek Voska Jelena Skibova Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success Journal of Diabetes Research |
| title | Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success |
| title_full | Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success |
| title_fullStr | Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success |
| title_full_unstemmed | Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success |
| title_short | Combining Donor Characteristics with Immunohistological Data Improves the Prediction of Islet Isolation Success |
| title_sort | combining donor characteristics with immunohistological data improves the prediction of islet isolation success |
| url | http://dx.doi.org/10.1155/2016/4214328 |
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