A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy
Background. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy.Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung ti...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2024-11-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/1158 |
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| author | T. A. Bogush A. N. Grishanina A. M. Scherbakov D. A. Khochenkov R. Yu. Yunusova S. A. Kalyuzhny V. S. Kosorukov |
| author_facet | T. A. Bogush A. N. Grishanina A. M. Scherbakov D. A. Khochenkov R. Yu. Yunusova S. A. Kalyuzhny V. S. Kosorukov |
| author_sort | T. A. Bogush |
| collection | DOAJ |
| description | Background. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy.Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung tissue with a characterized molecular phenotype by expression of one of the targets of immunotherapy — programmed cell death ligand 1 (PD-L1).Methods. PD-L1 expression was quantitatively analyzed by immunofluorescence method associated with flow cytometry.Results. A panel of lung cancer cell cultures of different histotypes and cells of normal lung tissue with characterized molecular phenotype was formed according to the expression of the immunotherapy target PD-L1. In terms of PD-L1 expression intensity, cell cultures can be arranged in a series: Calu-1 HFL-1 Calu-6 Wi-26 A-549 H-596 H-211, with a 7-fold difference in the index between Calu-1 and H-211.Conclusion. The panel of cultures of cancer and normal lung cells is recommended for the search and development of effective modifiers of the immunotherapy target PD-L1. |
| format | Article |
| id | doaj-art-a8a32253afab466a93d511098d19ca93 |
| institution | DOAJ |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-a8a32253afab466a93d511098d19ca932025-08-20T03:20:33ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902024-11-01697-8252910.37489/0235-2990-2024-69-7-8-25-291049A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of ImmunotherapyT. A. Bogush0A. N. Grishanina1A. M. Scherbakov2D. A. Khochenkov3R. Yu. Yunusova4S. A. Kalyuzhny5V. S. Kosorukov6N. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation; Gause Institute of New AntibioticsN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationN. N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationBackground. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy.Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung tissue with a characterized molecular phenotype by expression of one of the targets of immunotherapy — programmed cell death ligand 1 (PD-L1).Methods. PD-L1 expression was quantitatively analyzed by immunofluorescence method associated with flow cytometry.Results. A panel of lung cancer cell cultures of different histotypes and cells of normal lung tissue with characterized molecular phenotype was formed according to the expression of the immunotherapy target PD-L1. In terms of PD-L1 expression intensity, cell cultures can be arranged in a series: Calu-1 HFL-1 Calu-6 Wi-26 A-549 H-596 H-211, with a 7-fold difference in the index between Calu-1 and H-211.Conclusion. The panel of cultures of cancer and normal lung cells is recommended for the search and development of effective modifiers of the immunotherapy target PD-L1.https://www.antibiotics-chemotherapy.ru/jour/article/view/1158pd-l1immunotherapytumor cell panellung cancernormal lung fibroblasts |
| spellingShingle | T. A. Bogush A. N. Grishanina A. M. Scherbakov D. A. Khochenkov R. Yu. Yunusova S. A. Kalyuzhny V. S. Kosorukov A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy Антибиотики и Химиотерапия pd-l1 immunotherapy tumor cell panel lung cancer normal lung fibroblasts |
| title | A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy |
| title_full | A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy |
| title_fullStr | A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy |
| title_full_unstemmed | A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy |
| title_short | A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy |
| title_sort | panel of cultures of cancer and normal lung cells with a characterized expression of pd l1 to search and development of the target modifiers of immunotherapy |
| topic | pd-l1 immunotherapy tumor cell panel lung cancer normal lung fibroblasts |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/1158 |
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