Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia
Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulol...
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University of Brawijaya
2020-06-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/405 |
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| author | Mamluatul Faizah Tri Ardyati Suharjono Suharjono |
| author_facet | Mamluatul Faizah Tri Ardyati Suharjono Suharjono |
| author_sort | Mamluatul Faizah |
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| description | Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82 - 1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence. |
| format | Article |
| id | doaj-art-a8a1119ff8cc4d0f9b323951ee7c67bb |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | University of Brawijaya |
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| series | Journal of Experimental Life Science |
| spelling | doaj-art-a8a1119ff8cc4d0f9b323951ee7c67bb2025-08-20T02:30:50ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552020-06-01102132137https://doi.org/10.21776/ub.jels.2020.010.02.09Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, IndonesiaMamluatul Faizah0Tri Ardyati1Suharjono Suharjono2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaPalopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82 - 1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence.https://jels.ub.ac.id/index.php/jels/article/view/405burkholderia cepaciacellulolytic bacteriacellulase enzymesago waste. |
| spellingShingle | Mamluatul Faizah Tri Ardyati Suharjono Suharjono Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia Journal of Experimental Life Science burkholderia cepacia cellulolytic bacteria cellulase enzyme sago waste. |
| title | Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia |
| title_full | Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia |
| title_fullStr | Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia |
| title_full_unstemmed | Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia |
| title_short | Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia |
| title_sort | isolation and identification of indigenous cellulolytic bacteria from sago pith waste at palopo south sulawesi indonesia |
| topic | burkholderia cepacia cellulolytic bacteria cellulase enzyme sago waste. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/405 |
| work_keys_str_mv | AT mamluatulfaizah isolationandidentificationofindigenouscellulolyticbacteriafromsagopithwasteatpaloposouthsulawesiindonesia AT triardyati isolationandidentificationofindigenouscellulolyticbacteriafromsagopithwasteatpaloposouthsulawesiindonesia AT suharjonosuharjono isolationandidentificationofindigenouscellulolyticbacteriafromsagopithwasteatpaloposouthsulawesiindonesia |