Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep
Crimped wool in Tan sheep gradually transitions to straight wool after 35 days (the er-mao stage), which reduces its commercial value. To investigate the changes in wool proteins during this stage, we performed comparative proteomic analysis of the straight and crimped wool using tandem mass tag (TM...
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2024-10-01
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| author | An Shi Sijia Ma Zhuo Yang Wei Ding Jinyang Tian Xin Chen Jinzhong Tao |
| author_facet | An Shi Sijia Ma Zhuo Yang Wei Ding Jinyang Tian Xin Chen Jinzhong Tao |
| author_sort | An Shi |
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| description | Crimped wool in Tan sheep gradually transitions to straight wool after 35 days (the er-mao stage), which reduces its commercial value. To investigate the changes in wool proteins during this stage, we performed comparative proteomic analysis of the straight and crimped wool using tandem mass tag (TMT)-based quantification. The mean fur curvature (MFC) of crimped wool was significantly greater than that of straight wool (<i>p</i> < 0.001). We identified 1218 proteins between the two types of wool, including 50 keratins (Ks) and 10 keratin-associated proteins (KAPs). There were 213 differentially expressed proteins, including 13 Ks and 4 KAPs. Crimped wool showed relatively high abundances of KAP24-1, K84, K32, K82, and intermediate filament rod domain-containing protein (IRDC), whereas straight wool had relatively high abundances of K6A, K27, K80, KAP16-1, KAP27-1, and trichohyalin (TCHH). The expression levels of KAP16-1, KAP24-1, and KAP27-1 were related to the ratio of paracortex, which may be associated with wool crimp formation. Additionally, high expressions of TCHH, K27, and K6A in the inner root sheath (IRS) were linked to fiber fineness in straight wool. These findings provide insight into the overall expression and distribution patterns of Ks and KAPs, offering opportunities to improve wool quality and enhance its economic potential in the textile industry. |
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| spelling | doaj-art-a8919c6d206446ed926eb9fa06cff4d42025-08-20T01:47:41ZengMDPI AGAnimals2076-26152024-10-011419285810.3390/ani14192858Proteomic Analysis of Crimped and Straight Wool in Chinese Tan SheepAn Shi0Sijia Ma1Zhuo Yang2Wei Ding3Jinyang Tian4Xin Chen5Jinzhong Tao6College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaInstitute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, ChinaNingxia Yanchi Tan Sheep Breeding Center, Ningxia Department of Agriculture and Rural Affairs, Wuzhong 751506, ChinaNingxia Yanchi Tan Sheep Breeding Center, Ningxia Department of Agriculture and Rural Affairs, Wuzhong 751506, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCrimped wool in Tan sheep gradually transitions to straight wool after 35 days (the er-mao stage), which reduces its commercial value. To investigate the changes in wool proteins during this stage, we performed comparative proteomic analysis of the straight and crimped wool using tandem mass tag (TMT)-based quantification. The mean fur curvature (MFC) of crimped wool was significantly greater than that of straight wool (<i>p</i> < 0.001). We identified 1218 proteins between the two types of wool, including 50 keratins (Ks) and 10 keratin-associated proteins (KAPs). There were 213 differentially expressed proteins, including 13 Ks and 4 KAPs. Crimped wool showed relatively high abundances of KAP24-1, K84, K32, K82, and intermediate filament rod domain-containing protein (IRDC), whereas straight wool had relatively high abundances of K6A, K27, K80, KAP16-1, KAP27-1, and trichohyalin (TCHH). The expression levels of KAP16-1, KAP24-1, and KAP27-1 were related to the ratio of paracortex, which may be associated with wool crimp formation. Additionally, high expressions of TCHH, K27, and K6A in the inner root sheath (IRS) were linked to fiber fineness in straight wool. These findings provide insight into the overall expression and distribution patterns of Ks and KAPs, offering opportunities to improve wool quality and enhance its economic potential in the textile industry.https://www.mdpi.com/2076-2615/14/19/2858proteomickeratinkeratin-associated proteinproteomicscrimped wooltan sheep |
| spellingShingle | An Shi Sijia Ma Zhuo Yang Wei Ding Jinyang Tian Xin Chen Jinzhong Tao Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep Animals proteomic keratin keratin-associated protein proteomics crimped wool tan sheep |
| title | Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep |
| title_full | Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep |
| title_fullStr | Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep |
| title_full_unstemmed | Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep |
| title_short | Proteomic Analysis of Crimped and Straight Wool in Chinese Tan Sheep |
| title_sort | proteomic analysis of crimped and straight wool in chinese tan sheep |
| topic | proteomic keratin keratin-associated protein proteomics crimped wool tan sheep |
| url | https://www.mdpi.com/2076-2615/14/19/2858 |
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