Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis
Abstract Background The study aimed to was to investigate the relationship between miR-2861, miR-5011-5p, and colorectal carcinogenesis. Method In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was per...
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BMC
2025-01-01
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| Series: | BMC Medical Genomics |
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| Online Access: | https://doi.org/10.1186/s12920-024-02080-6 |
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| author | Alper Aytekin Hikmet Kadakal Deniz Mihcioglu Turkan Gurer |
| author_facet | Alper Aytekin Hikmet Kadakal Deniz Mihcioglu Turkan Gurer |
| author_sort | Alper Aytekin |
| collection | DOAJ |
| description | Abstract Background The study aimed to was to investigate the relationship between miR-2861, miR-5011-5p, and colorectal carcinogenesis. Method In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was performed qRT-PCR to determine the expression levels of miR‑2861 and miR‑5011-5p. In addition, it was predicted that dysregulated miRNAs targets, pathways and functional gene annotations that may be important in colorectal carcinogenesis using KEGG pathway and GO analysis. Results The resulting data revealed that both expression levels of miR-2861 and miR-5011-5p were significantly decreased in tumor tissues compared with non-tumor tissues of CRC patients. The GO and KEGG pathway analysis showed that miR-2861 and miR-5011-5p may participate in multiple the biological process, cellular components, and molecular function subcategories such as mitotic cell cycle, regulation of small GTPase mediated signal transduction, cell death, and acid binding transcription factor activity. It was also revealed that target genes of miRNAs can be found in signaling pathways such as TGF-beta, Rap1, Ras, cAMP, Wnt, mTOR and, PI3K-Akt signaling pathways. Conclusion These findings imply that miR-2861 and miR-5011-5p might function as tumor suppressors in the development of CRC. |
| format | Article |
| id | doaj-art-a88e5864b15e4569b9339f59fef159fa |
| institution | Kabale University |
| issn | 1755-8794 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj-art-a88e5864b15e4569b9339f59fef159fa2025-01-05T12:49:25ZengBMCBMC Medical Genomics1755-87942025-01-0118111210.1186/s12920-024-02080-6Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesisAlper Aytekin0Hikmet Kadakal1Deniz Mihcioglu2Turkan Gurer3Department of General Surgery, Faculty of Medicine, Gaziantep UniversityDepartment of Biology, Faculty of Art and Science, Gaziantep UniversityDepartment of Nutrition and Dietetics, Faculty of Health Science, SANKO UniversityDepartment of Biology, Faculty of Art and Science, Gaziantep UniversityAbstract Background The study aimed to was to investigate the relationship between miR-2861, miR-5011-5p, and colorectal carcinogenesis. Method In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was performed qRT-PCR to determine the expression levels of miR‑2861 and miR‑5011-5p. In addition, it was predicted that dysregulated miRNAs targets, pathways and functional gene annotations that may be important in colorectal carcinogenesis using KEGG pathway and GO analysis. Results The resulting data revealed that both expression levels of miR-2861 and miR-5011-5p were significantly decreased in tumor tissues compared with non-tumor tissues of CRC patients. The GO and KEGG pathway analysis showed that miR-2861 and miR-5011-5p may participate in multiple the biological process, cellular components, and molecular function subcategories such as mitotic cell cycle, regulation of small GTPase mediated signal transduction, cell death, and acid binding transcription factor activity. It was also revealed that target genes of miRNAs can be found in signaling pathways such as TGF-beta, Rap1, Ras, cAMP, Wnt, mTOR and, PI3K-Akt signaling pathways. Conclusion These findings imply that miR-2861 and miR-5011-5p might function as tumor suppressors in the development of CRC.https://doi.org/10.1186/s12920-024-02080-6Bioinformatics analysisColorectal cancermicroRNAmicroRNA expressionRT-qPCR |
| spellingShingle | Alper Aytekin Hikmet Kadakal Deniz Mihcioglu Turkan Gurer Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis BMC Medical Genomics Bioinformatics analysis Colorectal cancer microRNA microRNA expression RT-qPCR |
| title | Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis |
| title_full | Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis |
| title_fullStr | Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis |
| title_full_unstemmed | Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis |
| title_short | Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis |
| title_sort | bioinformatics analysis of mir 2861 and mir 5011 5p that function as potential tumor suppressors in colorectal carcinogenesis |
| topic | Bioinformatics analysis Colorectal cancer microRNA microRNA expression RT-qPCR |
| url | https://doi.org/10.1186/s12920-024-02080-6 |
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