Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage
Objective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing...
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| Format: | Article |
| Language: | English |
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Istanbul University Press
2023-06-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/803A0B5E0155420ABD802FDB3512DC2C |
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| author | Fatma Bedia Karakaya-çimen Çağlar Macit Güzin Göksun Sivas Tuğba Tunalı Akbay Göksel Şener Feriha Ercan |
| author_facet | Fatma Bedia Karakaya-çimen Çağlar Macit Güzin Göksun Sivas Tuğba Tunalı Akbay Göksel Şener Feriha Ercan |
| author_sort | Fatma Bedia Karakaya-çimen |
| collection | DOAJ |
| description | Objective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose of MTX dissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis samples were obtained. Seminiferous tubules and basement membrane were evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen’s score, the seminiferous tubule’s diameter, and the germinal epithelium’s thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress. |
| format | Article |
| id | doaj-art-a881634c623e41d3b8bef18bf1917a71 |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-a881634c623e41d3b8bef18bf1917a712025-08-20T02:10:39ZengIstanbul University PressEuropean Journal of Biology2618-61442023-06-01821313710.26650/EurJBiol.2023.1271825123456Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular DamageFatma Bedia Karakaya-çimen0https://orcid.org/0000-0001-6054-0752Çağlar Macit1https://orcid.org/0000-0002-5532-2395Güzin Göksun Sivas2https://orcid.org/0000-0001-7347-490XTuğba Tunalı Akbay3https://orcid.org/0000-0002-2091-9298Göksel Şener4https://orcid.org/0000-0001-7444-6193Feriha Ercan5https://orcid.org/0000-0003-2339-5669Marmara Üniversitesi, İstanbul, Türkiyeİstanbul Medipol Üniversitesi, Istanbul, TurkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeFenerbahçe Üniversitesi, Istanbul, TurkiyeMarmara Üniversitesi, İstanbul, TürkiyeObjective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types of cancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX. Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose of MTX dissolved in saline (20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis samples were obtained. Seminiferous tubules and basement membrane were evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured. Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen’s score, the seminiferous tubule’s diameter, and the germinal epithelium’s thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity. Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-induced testicular damage by inhibiting oxidative stress.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/803A0B5E0155420ABD802FDB3512DC2Cmethotrexatepanax ginsengtestisoxidative stress |
| spellingShingle | Fatma Bedia Karakaya-çimen Çağlar Macit Güzin Göksun Sivas Tuğba Tunalı Akbay Göksel Şener Feriha Ercan Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage European Journal of Biology methotrexate panax ginseng testis oxidative stress |
| title | Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage |
| title_full | Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage |
| title_fullStr | Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage |
| title_full_unstemmed | Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage |
| title_short | Morphological and Biochemical Investigation of the Protective Effects of Panax ginseng on Methotrexate-Induced Testicular Damage |
| title_sort | morphological and biochemical investigation of the protective effects of panax ginseng on methotrexate induced testicular damage |
| topic | methotrexate panax ginseng testis oxidative stress |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/803A0B5E0155420ABD802FDB3512DC2C |
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