Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion
Enzymatic conversion is a promising option for utilizing CO2 as a renewable C1 source for high-value chemicals due to the high selectivity and specificity of enzymes, their environmental friendliness, and their ability to operate under mild conditions. The present study addresses CO2 conversion by d...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Results in Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590123025005201 |
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| author | Diego Maureira Sady Roberto Rodriguez Oscar Romero Marina Guillén Gregorio Álvaro Lorena Wilson Carminna Ottone |
| author_facet | Diego Maureira Sady Roberto Rodriguez Oscar Romero Marina Guillén Gregorio Álvaro Lorena Wilson Carminna Ottone |
| author_sort | Diego Maureira |
| collection | DOAJ |
| description | Enzymatic conversion is a promising option for utilizing CO2 as a renewable C1 source for high-value chemicals due to the high selectivity and specificity of enzymes, their environmental friendliness, and their ability to operate under mild conditions. The present study addresses CO2 conversion by developing a novel biocatalyst for formic acid production. Immobilization of formate dehydrogenase (FDH) from Candida boidinii onto carbon felt (CF) using an affinity binding approach is explored. Pre-treatment of CF with HNO3 increased nickel content with respect to the untreated and KOH treated CF samples, resulting in a biocatalyst with the highest specific activity. The selected biocatalyst achieved a promising formic acid yield of 78 % under pressurized CO2 conditions. Utilizing an 8-bar pressurized reactor led to a productivity of 0.56 mol L⁻¹ h⁻¹. The biocatalyst efficiently catalyzed formic acid synthesis over four consecutive reaction cycles, but its efficiency decreased with each cycle. This research demonstrates the feasibility of simple and efficient FDH immobilization on CF for CO2 conversion. The resulting biocatalyst shows potential for formic acid production, but further investigation is needed to enhance reusability for industrial applications. |
| format | Article |
| id | doaj-art-a875419012cb4f7cbde03edb50dc519f |
| institution | OA Journals |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Engineering |
| spelling | doaj-art-a875419012cb4f7cbde03edb50dc519f2025-08-20T02:01:15ZengElsevierResults in Engineering2590-12302025-03-012510444210.1016/j.rineng.2025.104442Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversionDiego Maureira0Sady Roberto Rodriguez1Oscar Romero2Marina Guillén3Gregorio Álvaro4Lorena Wilson5Carminna Ottone6Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, ChileBioprocess Engineering and Applied Biocatalysis Group, Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainBioprocess Engineering and Applied Biocatalysis Group, Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain; Corresponding author at: Bioprocess Engineering and Applied Biocatalysis Group, Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.Bioprocess Engineering and Applied Biocatalysis Group, Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainBioprocess Engineering and Applied Biocatalysis Group, Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile; Corresponding author at: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile.Enzymatic conversion is a promising option for utilizing CO2 as a renewable C1 source for high-value chemicals due to the high selectivity and specificity of enzymes, their environmental friendliness, and their ability to operate under mild conditions. The present study addresses CO2 conversion by developing a novel biocatalyst for formic acid production. Immobilization of formate dehydrogenase (FDH) from Candida boidinii onto carbon felt (CF) using an affinity binding approach is explored. Pre-treatment of CF with HNO3 increased nickel content with respect to the untreated and KOH treated CF samples, resulting in a biocatalyst with the highest specific activity. The selected biocatalyst achieved a promising formic acid yield of 78 % under pressurized CO2 conditions. Utilizing an 8-bar pressurized reactor led to a productivity of 0.56 mol L⁻¹ h⁻¹. The biocatalyst efficiently catalyzed formic acid synthesis over four consecutive reaction cycles, but its efficiency decreased with each cycle. This research demonstrates the feasibility of simple and efficient FDH immobilization on CF for CO2 conversion. The resulting biocatalyst shows potential for formic acid production, but further investigation is needed to enhance reusability for industrial applications.http://www.sciencedirect.com/science/article/pii/S2590123025005201Carbon dioxideFormic acidFormate dehydrogenaseImmobilizationCarbon felt |
| spellingShingle | Diego Maureira Sady Roberto Rodriguez Oscar Romero Marina Guillén Gregorio Álvaro Lorena Wilson Carminna Ottone Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion Results in Engineering Carbon dioxide Formic acid Formate dehydrogenase Immobilization Carbon felt |
| title | Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion |
| title_full | Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion |
| title_fullStr | Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion |
| title_full_unstemmed | Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion |
| title_short | Immobilization of FDH on carbon felt by affinity binding strategy for CO2 conversion |
| title_sort | immobilization of fdh on carbon felt by affinity binding strategy for co2 conversion |
| topic | Carbon dioxide Formic acid Formate dehydrogenase Immobilization Carbon felt |
| url | http://www.sciencedirect.com/science/article/pii/S2590123025005201 |
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