Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul
The aim of the study was to determine the prevalence rates of respiratory pathogens using syndromic tests and also to show which respiratory viruses were detected in suspected cases, especially during and after the pandemic period. A total of 1984 different respiratory tract samples from various dep...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/6/776 |
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| author | Mustafa Onel Hayriye Kırkoyun Uysal Arat Hulikyan Yasemin Ayse Ucar Gizem Yapar Aytaj Allahverdiyeva Serra Zeynep Akkoyunlu Eray Yurtseven Mehmet Demirci Sevim Mese Ali Agacfidan |
| author_facet | Mustafa Onel Hayriye Kırkoyun Uysal Arat Hulikyan Yasemin Ayse Ucar Gizem Yapar Aytaj Allahverdiyeva Serra Zeynep Akkoyunlu Eray Yurtseven Mehmet Demirci Sevim Mese Ali Agacfidan |
| author_sort | Mustafa Onel |
| collection | DOAJ |
| description | The aim of the study was to determine the prevalence rates of respiratory pathogens using syndromic tests and also to show which respiratory viruses were detected in suspected cases, especially during and after the pandemic period. A total of 1984 different respiratory tract samples from various departments were included and studied with the QIAstat-Dx device in 2021–2023. The samples were studied with the QIAstat-Dx1 Respiratory SARS-CoV-2 Panel. The kit used was a fully automated, multiplex syndromic test that detected SARS-CoV-2 and 21 other respiratory tract pathogens. As a result of the study, the prevalence of Rhinovirus/Enterovirus (RV/EV) (18.59%), RV/EV-SARS-CoV-2 (42.74%), SARS-CoV-2 (5.04%), and Influenza A Virus (IAV) (5.59%) agents was found to be higher than other agents during the period investigated. Among the 1984 patients examined, 959 (48.33%) had a single viral agent, 156 (7.86%) had double coinfection, 11 (0.55%) had triple coinfection and 1 patient had quadruple coinfection. Nearly half of the patients had a straightforward infection, which helps clinicians in directing specific treatment methods. The study results demonstrate that during the pandemic period, the detection of respiratory pathogens such as SARS-CoV-2 and RV/EV was not only critical for accurate diagnosis but also served as an important indicator of the broader epidemiological trends in respiratory infections. The seasonal distribution showed that while RV/EV was frequently present, its coinfection with SARS-CoV-2 was notably observed only in the first trimester. In light of our findings showing high rates of SARS-CoV-2 and RV/EV detection, along with diverse patterns of coinfection in clinical samples, such comprehensive testing not only assists in rapid diagnosis but also informs public health strategies by reflecting the evolving landscape of respiratory infections in the pandemic and post-pandemic era. |
| format | Article |
| id | doaj-art-a8713ca11d6649c883e94e9f3e176ed4 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-a8713ca11d6649c883e94e9f3e176ed42025-08-20T03:29:49ZengMDPI AGViruses1999-49152025-05-0117677610.3390/v17060776Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in IstanbulMustafa Onel0Hayriye Kırkoyun Uysal1Arat Hulikyan2Yasemin Ayse Ucar3Gizem Yapar4Aytaj Allahverdiyeva5Serra Zeynep Akkoyunlu6Eray Yurtseven7Mehmet Demirci8Sevim Mese9Ali Agacfidan10Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Istanbul Beykent University, Istanbul 34520, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Istanbul Aydın University, Istanbul 34295, TurkeyDepartment of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Biostatistics, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Biostatistics, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Kırklareli University, Kırklareli 39100, TurkeyDepartment of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyDepartment of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TurkeyThe aim of the study was to determine the prevalence rates of respiratory pathogens using syndromic tests and also to show which respiratory viruses were detected in suspected cases, especially during and after the pandemic period. A total of 1984 different respiratory tract samples from various departments were included and studied with the QIAstat-Dx device in 2021–2023. The samples were studied with the QIAstat-Dx1 Respiratory SARS-CoV-2 Panel. The kit used was a fully automated, multiplex syndromic test that detected SARS-CoV-2 and 21 other respiratory tract pathogens. As a result of the study, the prevalence of Rhinovirus/Enterovirus (RV/EV) (18.59%), RV/EV-SARS-CoV-2 (42.74%), SARS-CoV-2 (5.04%), and Influenza A Virus (IAV) (5.59%) agents was found to be higher than other agents during the period investigated. Among the 1984 patients examined, 959 (48.33%) had a single viral agent, 156 (7.86%) had double coinfection, 11 (0.55%) had triple coinfection and 1 patient had quadruple coinfection. Nearly half of the patients had a straightforward infection, which helps clinicians in directing specific treatment methods. The study results demonstrate that during the pandemic period, the detection of respiratory pathogens such as SARS-CoV-2 and RV/EV was not only critical for accurate diagnosis but also served as an important indicator of the broader epidemiological trends in respiratory infections. The seasonal distribution showed that while RV/EV was frequently present, its coinfection with SARS-CoV-2 was notably observed only in the first trimester. In light of our findings showing high rates of SARS-CoV-2 and RV/EV detection, along with diverse patterns of coinfection in clinical samples, such comprehensive testing not only assists in rapid diagnosis but also informs public health strategies by reflecting the evolving landscape of respiratory infections in the pandemic and post-pandemic era.https://www.mdpi.com/1999-4915/17/6/776SARS-CoV-2respiratory infectionrespiratory virusesQIAstat-Dx1 respiratory SARS-CoV-2 panel |
| spellingShingle | Mustafa Onel Hayriye Kırkoyun Uysal Arat Hulikyan Yasemin Ayse Ucar Gizem Yapar Aytaj Allahverdiyeva Serra Zeynep Akkoyunlu Eray Yurtseven Mehmet Demirci Sevim Mese Ali Agacfidan Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul Viruses SARS-CoV-2 respiratory infection respiratory viruses QIAstat-Dx1 respiratory SARS-CoV-2 panel |
| title | Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul |
| title_full | Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul |
| title_fullStr | Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul |
| title_full_unstemmed | Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul |
| title_short | Syndromic Testing in the Pandemic Era and Beyond: Rapid Detection for Respiratory Infections in Istanbul |
| title_sort | syndromic testing in the pandemic era and beyond rapid detection for respiratory infections in istanbul |
| topic | SARS-CoV-2 respiratory infection respiratory viruses QIAstat-Dx1 respiratory SARS-CoV-2 panel |
| url | https://www.mdpi.com/1999-4915/17/6/776 |
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