Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies
Fermented milk (FM) is well-known to confer health-promoting benefits, particularly for managing chronic metabolic diseases. However, the specific cholesterol esterase (CE) inhibitory activities of FM produced from different animal milk sources have not been extensively explored. This study for the...
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Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524008861 |
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| author | Feyisola Fisayo Ajayi Fatimah AlShebli Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood Priti Mudgil |
| author_facet | Feyisola Fisayo Ajayi Fatimah AlShebli Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood Priti Mudgil |
| author_sort | Feyisola Fisayo Ajayi |
| collection | DOAJ |
| description | Fermented milk (FM) is well-known to confer health-promoting benefits, particularly for managing chronic metabolic diseases. However, the specific cholesterol esterase (CE) inhibitory activities of FM produced from different animal milk sources have not been extensively explored. This study for the first time investigates the CE inhibition potential of FM derived from bovine (F_BM), camel (F_CM), sheep (F_SM), and goat milk (F_GM), each fermented with five different probiotic strains and stored for 14 days under refrigeration. Further, peptides identification was performed and in silico approaches were used to dock potent peptides with CE enzyme (PDB: 1AQL) to decipher mechanism of enzyme inhibition. Comprehensive approach of this study combined CE inhibition assays, peptide identification, and in silico molecular docking with the CE enzyme (PDB: 1AQL) to elucidate mechanisms underlying enzyme inhibition. Upon fermentation improvements in CE-inhibition (lower IC50 values) were observed compared to non-fermented counterparts. Moreover, the CE-inhibition potency of the FM varies significantly among the milk types and probiotic strain (p < 0.05). Regardless of probiotic strains, CE-inhibition was more evident for F_GM followed by F_CM. Peptide sequencing and molecular docking studies revealed APSFSDIPNPIGSENSEKTTMPLW from F_BM showed potent binding to CE's active site, while peptides from F_CM, F_SM, and F_GM showed indirect CE-inhibitory mechanisms. These findings suggest potential anti-hypercholesteremic effects of bovine and non-bovine fermented milk, indicating their potential use in developing novel dairy products with hypolipidemic activities. |
| format | Article |
| id | doaj-art-a870e98da7e845a2ad7f091befd01877 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-a870e98da7e845a2ad7f091befd018772025-08-20T01:59:31ZengElsevierFood Chemistry: X2590-15752024-12-012410199810.1016/j.fochx.2024.101998Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studiesFeyisola Fisayo Ajayi0Fatimah AlShebli1Pei-Gee Yap2Chee-Yuen Gan3Sajid Maqsood4Priti Mudgil5Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al-Ain 15551, United Arab EmiratesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al-Ain 15551, United Arab EmiratesAnalytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800 USM, Penang, MalaysiaAnalytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800 USM, Penang, MalaysiaDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al-Ain 15551, United Arab Emirates; Zayed Centre of Health Science, United Arab Emirates University, Al-Ain 15551, United Arab Emirates; Corresponding author at: Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al-Ain 15551, United Arab Emirates; Corresponding author.Fermented milk (FM) is well-known to confer health-promoting benefits, particularly for managing chronic metabolic diseases. However, the specific cholesterol esterase (CE) inhibitory activities of FM produced from different animal milk sources have not been extensively explored. This study for the first time investigates the CE inhibition potential of FM derived from bovine (F_BM), camel (F_CM), sheep (F_SM), and goat milk (F_GM), each fermented with five different probiotic strains and stored for 14 days under refrigeration. Further, peptides identification was performed and in silico approaches were used to dock potent peptides with CE enzyme (PDB: 1AQL) to decipher mechanism of enzyme inhibition. Comprehensive approach of this study combined CE inhibition assays, peptide identification, and in silico molecular docking with the CE enzyme (PDB: 1AQL) to elucidate mechanisms underlying enzyme inhibition. Upon fermentation improvements in CE-inhibition (lower IC50 values) were observed compared to non-fermented counterparts. Moreover, the CE-inhibition potency of the FM varies significantly among the milk types and probiotic strain (p < 0.05). Regardless of probiotic strains, CE-inhibition was more evident for F_GM followed by F_CM. Peptide sequencing and molecular docking studies revealed APSFSDIPNPIGSENSEKTTMPLW from F_BM showed potent binding to CE's active site, while peptides from F_CM, F_SM, and F_GM showed indirect CE-inhibitory mechanisms. These findings suggest potential anti-hypercholesteremic effects of bovine and non-bovine fermented milk, indicating their potential use in developing novel dairy products with hypolipidemic activities.http://www.sciencedirect.com/science/article/pii/S2590157524008861Dairy proteinsBioactive peptidesFermentationProbioticsCholesteryl esterase inhibitionObesity |
| spellingShingle | Feyisola Fisayo Ajayi Fatimah AlShebli Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood Priti Mudgil Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies Food Chemistry: X Dairy proteins Bioactive peptides Fermentation Probiotics Cholesteryl esterase inhibition Obesity |
| title | Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies |
| title_full | Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies |
| title_fullStr | Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies |
| title_full_unstemmed | Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies |
| title_short | Assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro, in silico, and molecular docking studies |
| title_sort | assessment of hypolipidemic potential of cholesteryl esterase inhibitory peptides in different probiotic fermented milk through in vitro in silico and molecular docking studies |
| topic | Dairy proteins Bioactive peptides Fermentation Probiotics Cholesteryl esterase inhibition Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524008861 |
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