Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models
Background: The inflammatory phase of acute burns can last for 7 days. Meanwhile, severe burns may exhibit persistent inflammation long after the initial tissue damage. Negative pressure wound therapy (NPWT) is recommended to treat various lesions, including burns. This study aimed to compare the nu...
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KeAi Communications Co. Ltd.
2024-12-01
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| Series: | Chinese Journal of Plastic and Reconstructive Surgery |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2096691124000827 |
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| author | Giri Yurista Nabilah Anisa Novebri Irianiwati Muhammad Rosadi Seswandhana |
| author_facet | Giri Yurista Nabilah Anisa Novebri Irianiwati Muhammad Rosadi Seswandhana |
| author_sort | Giri Yurista |
| collection | DOAJ |
| description | Background: The inflammatory phase of acute burns can last for 7 days. Meanwhile, severe burns may exhibit persistent inflammation long after the initial tissue damage. Negative pressure wound therapy (NPWT) is recommended to treat various lesions, including burns. This study aimed to compare the number of inflammatory cells on days 3, 14, and 21 in deep dermal burns treated with two different therapies. Methods: This experimental study was conducted at the Prof. Soeparwi Veterinary Hospital, Yogyakarta, from February to September 2022. A total of 36 wounds created in 10-kg Yorkshire pig burn models were subjected to one of the following treatments: 0.9% sodium chloride or NPWT. These wounds were then sampled as paraffin blocks for microscopic inflammatory cell counts by an anatomical pathology specialist. Comparative analyses of lymphocytes, neutrophils, and plasma cells were performed using IBM SPSS version 22 for Windows. Results: A significant difference was found only in neutrophil counts on day 3 (5.30 ± 5.41), day 14 (42.83 ± 38.58), and day 21 (9.80 ± 8.97) (P=0.009), with notable changes from day 3 to day 14 (37.53 ± 40.51; P=0.043) and day 14 to day 21 (−33.03 ± 42.79; P=0.049). Meanwhile, no significant differences were found in either lymphocytes or plasma cells between days in either treatment group. Conclusion: NPWT effectively reduced neutrophil counts on day 21 of treatment, indicating its beneficial effects in preventing prolonged inflammation during wound healing. This demonstrates its potential as an alternative dressing therapy for deep dermal burn injuries. |
| format | Article |
| id | doaj-art-a869706116af474b85d2dc73b4ac5922 |
| institution | Kabale University |
| issn | 2096-6911 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Chinese Journal of Plastic and Reconstructive Surgery |
| spelling | doaj-art-a869706116af474b85d2dc73b4ac59222024-12-08T06:09:41ZengKeAi Communications Co. Ltd.Chinese Journal of Plastic and Reconstructive Surgery2096-69112024-12-0164166171Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound modelsGiri Yurista0Nabilah Anisa Novebri1 Irianiwati2Muhammad Rosadi Seswandhana3Department of Surgery, Dr. Sardjito Hospital/Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Surgery, Dr. Sardjito Hospital/Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Anatomical Pathology, Dr. Sardjito Hospital/Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Surgery, Dr. Sardjito Hospital/Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Corresponding author. Division of Plastic, Reconstructive, and Aesthetic Surgery, Department of Surgery, Dr. Sardjito Hospital/Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Jl. Kesehatan Sendowo No. 1, Sendowo, Sinduadi, Mlati, Sleman Regency, Special Region of Yogyakarta, 55281, Indonesia.Background: The inflammatory phase of acute burns can last for 7 days. Meanwhile, severe burns may exhibit persistent inflammation long after the initial tissue damage. Negative pressure wound therapy (NPWT) is recommended to treat various lesions, including burns. This study aimed to compare the number of inflammatory cells on days 3, 14, and 21 in deep dermal burns treated with two different therapies. Methods: This experimental study was conducted at the Prof. Soeparwi Veterinary Hospital, Yogyakarta, from February to September 2022. A total of 36 wounds created in 10-kg Yorkshire pig burn models were subjected to one of the following treatments: 0.9% sodium chloride or NPWT. These wounds were then sampled as paraffin blocks for microscopic inflammatory cell counts by an anatomical pathology specialist. Comparative analyses of lymphocytes, neutrophils, and plasma cells were performed using IBM SPSS version 22 for Windows. Results: A significant difference was found only in neutrophil counts on day 3 (5.30 ± 5.41), day 14 (42.83 ± 38.58), and day 21 (9.80 ± 8.97) (P=0.009), with notable changes from day 3 to day 14 (37.53 ± 40.51; P=0.043) and day 14 to day 21 (−33.03 ± 42.79; P=0.049). Meanwhile, no significant differences were found in either lymphocytes or plasma cells between days in either treatment group. Conclusion: NPWT effectively reduced neutrophil counts on day 21 of treatment, indicating its beneficial effects in preventing prolonged inflammation during wound healing. This demonstrates its potential as an alternative dressing therapy for deep dermal burn injuries.http://www.sciencedirect.com/science/article/pii/S2096691124000827Burn woundsLymphocytesNegative pressure wound therapyNeutrophilsPlasma cellsWound healing |
| spellingShingle | Giri Yurista Nabilah Anisa Novebri Irianiwati Muhammad Rosadi Seswandhana Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models Chinese Journal of Plastic and Reconstructive Surgery Burn wounds Lymphocytes Negative pressure wound therapy Neutrophils Plasma cells Wound healing |
| title | Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| title_full | Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| title_fullStr | Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| title_full_unstemmed | Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| title_short | Impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| title_sort | impact of negative pressure wound therapy on inflammatory cell counts in porcine deep dermal burn wound models |
| topic | Burn wounds Lymphocytes Negative pressure wound therapy Neutrophils Plasma cells Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2096691124000827 |
| work_keys_str_mv | AT giriyurista impactofnegativepressurewoundtherapyoninflammatorycellcountsinporcinedeepdermalburnwoundmodels AT nabilahanisanovebri impactofnegativepressurewoundtherapyoninflammatorycellcountsinporcinedeepdermalburnwoundmodels AT irianiwati impactofnegativepressurewoundtherapyoninflammatorycellcountsinporcinedeepdermalburnwoundmodels AT muhammadrosadiseswandhana impactofnegativepressurewoundtherapyoninflammatorycellcountsinporcinedeepdermalburnwoundmodels |