Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads
Background: Food-borne diseases are one of the major problems in developing countries. This study aims to investigate the prevalence, antibiotic resistance pattern, and serotyping of Shigella isolates from Olivier salad. Methods: 150 samples of Olivier salad, including 50 industrial samples from 10...
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Shahid Sadoughi University of Medical Sciences and Health Services
2025-02-01
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| Series: | Journal of Nutrition and Food Security |
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| Online Access: | http://jnfs.ssu.ac.ir/article-1-998-en.pdf |
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| author | Shiva Jahanbakhshi Mohammad Mehdi Soltan Dallal Abbas Rahimi Foroushani Katayoun Samimi-Rad Seyedeh Zohre Mirbagheri Ahmad Nasser Mohammad Reza Mohammadi |
| author_facet | Shiva Jahanbakhshi Mohammad Mehdi Soltan Dallal Abbas Rahimi Foroushani Katayoun Samimi-Rad Seyedeh Zohre Mirbagheri Ahmad Nasser Mohammad Reza Mohammadi |
| author_sort | Shiva Jahanbakhshi |
| collection | DOAJ |
| description | Background: Food-borne diseases are one of the major problems in developing countries. This study aims to investigate the prevalence, antibiotic resistance pattern, and serotyping of Shigella isolates from Olivier salad. Methods: 150 samples of Olivier salad, including 50 industrial samples from 10 different available brands in Tehran's shops and 100 traditional samples from Tehran's southern regions, were randomly obtained between April to October of 2021. These samples were examined for bacterial isolation and identification, which was finally confirmed by the API-20E kit. Then, a serological reaction was used to confirm Shigella and determine the species. The antibiotic resistance pattern of isolates was evaluated according to Clinical and Laboratory Standards Institute (CLSI, 2021) instructions by the Kirby-Bauer method. Results: Among these samples, 10 isolates (6.6%) of Shigella were isolated, of which 6 (4%) belonged to serogroup D (Shigella sonnei), 3 (2%) to serogroup B (Shigella flexneri), and 1 isolate (0.66%) belonged to serogroup C (Shigella boydii). This study showed that all Shigella isolates were related to traditional Olivier salad and were not Shigella spp. isolated from the industrial salad. Other bacteria isolated from traditional salad included Escherichia coli, Salmonella, Pseudomonas, Klebsiella, and Citrobacter. Multidrug resistance (MDR) was not observed in all isolates, and among 10 isolates of Shigella, 40% showed complete resistance to ampicillin, but other isolates showed 60% intermediate resistance to this antibiotic. No resistance was observed for other tested antibiotics. Conclusion: The difference between the level of contamination in both traditional and industrial samples of salad well indicates familiarity with sanitary protocols and standards provided to reduce the microbial load. |
| format | Article |
| id | doaj-art-a8690dffbe7342af8a3ce24b9eaeca28 |
| institution | Kabale University |
| issn | 2476-7417 2476-7425 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Shahid Sadoughi University of Medical Sciences and Health Services |
| record_format | Article |
| series | Journal of Nutrition and Food Security |
| spelling | doaj-art-a8690dffbe7342af8a3ce24b9eaeca282025-02-08T10:57:36ZengShahid Sadoughi University of Medical Sciences and Health ServicesJournal of Nutrition and Food Security2476-74172476-74252025-02-011014249Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier SaladsShiva Jahanbakhshi0Mohammad Mehdi Soltan Dallal1Abbas Rahimi Foroushani2Katayoun Samimi-Rad3Seyedeh Zohre Mirbagheri4Ahmad Nasser5Mohammad Reza Mohammadi6 Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Virology, School of Public Health, Tehran University of Medical Science, Tehran, Iran Department of Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 6Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: Food-borne diseases are one of the major problems in developing countries. This study aims to investigate the prevalence, antibiotic resistance pattern, and serotyping of Shigella isolates from Olivier salad. Methods: 150 samples of Olivier salad, including 50 industrial samples from 10 different available brands in Tehran's shops and 100 traditional samples from Tehran's southern regions, were randomly obtained between April to October of 2021. These samples were examined for bacterial isolation and identification, which was finally confirmed by the API-20E kit. Then, a serological reaction was used to confirm Shigella and determine the species. The antibiotic resistance pattern of isolates was evaluated according to Clinical and Laboratory Standards Institute (CLSI, 2021) instructions by the Kirby-Bauer method. Results: Among these samples, 10 isolates (6.6%) of Shigella were isolated, of which 6 (4%) belonged to serogroup D (Shigella sonnei), 3 (2%) to serogroup B (Shigella flexneri), and 1 isolate (0.66%) belonged to serogroup C (Shigella boydii). This study showed that all Shigella isolates were related to traditional Olivier salad and were not Shigella spp. isolated from the industrial salad. Other bacteria isolated from traditional salad included Escherichia coli, Salmonella, Pseudomonas, Klebsiella, and Citrobacter. Multidrug resistance (MDR) was not observed in all isolates, and among 10 isolates of Shigella, 40% showed complete resistance to ampicillin, but other isolates showed 60% intermediate resistance to this antibiotic. No resistance was observed for other tested antibiotics. Conclusion: The difference between the level of contamination in both traditional and industrial samples of salad well indicates familiarity with sanitary protocols and standards provided to reduce the microbial load.http://jnfs.ssu.ac.ir/article-1-998-en.pdfshigellasaladsdrug resistancebacterial antibiotic resistancefoodborne diseases. |
| spellingShingle | Shiva Jahanbakhshi Mohammad Mehdi Soltan Dallal Abbas Rahimi Foroushani Katayoun Samimi-Rad Seyedeh Zohre Mirbagheri Ahmad Nasser Mohammad Reza Mohammadi Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads Journal of Nutrition and Food Security shigella salads drug resistance bacterial antibiotic resistance foodborne diseases. |
| title | Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads |
| title_full | Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads |
| title_fullStr | Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads |
| title_full_unstemmed | Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads |
| title_short | Investigating the Prevalence, Antibiotic Resistance Pattern, and Serotyping of Shigella Isolates from Traditional and Industrial Olivier Salads |
| title_sort | investigating the prevalence antibiotic resistance pattern and serotyping of shigella isolates from traditional and industrial olivier salads |
| topic | shigella salads drug resistance bacterial antibiotic resistance foodborne diseases. |
| url | http://jnfs.ssu.ac.ir/article-1-998-en.pdf |
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