Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry
Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0–10.0 immobilized pH gradient (IPG) strips. Comparative elucidation of differ...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/1049462 |
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| author | Pramod Kumar Singh Nidhi Shrivastava Krishna Chaturvedi Bechan Sharma Sameer S. Bhagyawant |
| author_facet | Pramod Kumar Singh Nidhi Shrivastava Krishna Chaturvedi Bechan Sharma Sameer S. Bhagyawant |
| author_sort | Pramod Kumar Singh |
| collection | DOAJ |
| description | Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0–10.0 immobilized pH gradient (IPG) strips. Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry. A total of 600 protein spots were detected in these accessions. In-gel protein expression patterns revealed three protein spots as upregulated and three other as downregulated. Using trypsin in-gel digestion, these differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) which showed 45% amino acid homology of chickpea seed storage proteins with Arabidopsis thaliana. |
| format | Article |
| id | doaj-art-a8671b85ec9047f8b9dcc7571bf63d53 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-a8671b85ec9047f8b9dcc7571bf63d532025-08-20T03:38:54ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/10494621049462Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass SpectrometryPramod Kumar Singh0Nidhi Shrivastava1Krishna Chaturvedi2Bechan Sharma3Sameer S. Bhagyawant4School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, IndiaDepartment of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, IndiaDefense Research & Development Establishment, Gwalior 474011, IndiaDepartment of Biochemistry, University of Allahabad, Allahabad 211002, IndiaSchool of Studies in Biotechnology, Jiwaji University, Gwalior 474011, IndiaProteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0–10.0 immobilized pH gradient (IPG) strips. Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry. A total of 600 protein spots were detected in these accessions. In-gel protein expression patterns revealed three protein spots as upregulated and three other as downregulated. Using trypsin in-gel digestion, these differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) which showed 45% amino acid homology of chickpea seed storage proteins with Arabidopsis thaliana.http://dx.doi.org/10.1155/2016/1049462 |
| spellingShingle | Pramod Kumar Singh Nidhi Shrivastava Krishna Chaturvedi Bechan Sharma Sameer S. Bhagyawant Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry Biochemistry Research International |
| title | Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry |
| title_full | Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry |
| title_fullStr | Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry |
| title_full_unstemmed | Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry |
| title_short | Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry |
| title_sort | characterization of seed storage proteins from chickpea using 2d electrophoresis coupled with mass spectrometry |
| url | http://dx.doi.org/10.1155/2016/1049462 |
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