Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence
Koi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this researc...
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University of Brawijaya
2018-02-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/246 |
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| author | Amira Baihani Uun Yanuhar Maftuch Maftuch |
| author_facet | Amira Baihani Uun Yanuhar Maftuch Maftuch |
| author_sort | Amira Baihani |
| collection | DOAJ |
| description | Koi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this research aims to understand the genetic variation and kindship of KHV isolate in the carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province based on thymidine kinase sequence. Water quality of fish pond was checked. KHV clinical symptom was checked visually in each fish sample, then sacrifices the organ (gill) for PCR analysis and DNA sequencing. The research results show that clinical symptoms that appear are pale fish gill and bleeding on the surface of the body. The temperature in the research site shows an optimum range for KHV development. The results electrophoresis of carp suspected with KHV shows five positives out of a total six samples. Genetically, KHV isolates from Lombok Island of West Nusa Tenggara Province is identical with the GenBank isolate code KHV-J/U/I and Indo_0C05WNT2. |
| format | Article |
| id | doaj-art-a86416b40baf4eb8ae28aa477bc7c402 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-a86416b40baf4eb8ae28aa477bc7c4022025-08-20T01:51:31ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552018-02-01813036https://doi.org/10.21776/ub.jels.2018.008.01.05Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase SequenceAmira Baihani0Uun Yanuhar1Maftuch Maftuch2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaKoi herpesvirus (KHV) is a virus that usually infects carp and koi causing mass mortality, as well as economic and social loss. The results of varied test are often found in its development. Allegedly, there are mutation or genetic variation of KHV in Indonesia. Based on these problems, this research aims to understand the genetic variation and kindship of KHV isolate in the carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province based on thymidine kinase sequence. Water quality of fish pond was checked. KHV clinical symptom was checked visually in each fish sample, then sacrifices the organ (gill) for PCR analysis and DNA sequencing. The research results show that clinical symptoms that appear are pale fish gill and bleeding on the surface of the body. The temperature in the research site shows an optimum range for KHV development. The results electrophoresis of carp suspected with KHV shows five positives out of a total six samples. Genetically, KHV isolates from Lombok Island of West Nusa Tenggara Province is identical with the GenBank isolate code KHV-J/U/I and Indo_0C05WNT2.https://jels.ub.ac.id/index.php/jels/article/view/246carpkoi herpesvirusphylogeneticthymidine kinase |
| spellingShingle | Amira Baihani Uun Yanuhar Maftuch Maftuch Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence Journal of Experimental Life Science carp koi herpesvirus phylogenetic thymidine kinase |
| title | Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence |
| title_full | Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence |
| title_fullStr | Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence |
| title_full_unstemmed | Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence |
| title_short | Analysis of Genotype and Phylogenetic of Koi Herpesvirus (KHV) on Carp (Cyprinus carpio) in Lombok Island of West Nusa Tenggara Province Based on Thymidine Kinase Sequence |
| title_sort | analysis of genotype and phylogenetic of koi herpesvirus khv on carp cyprinus carpio in lombok island of west nusa tenggara province based on thymidine kinase sequence |
| topic | carp koi herpesvirus phylogenetic thymidine kinase |
| url | https://jels.ub.ac.id/index.php/jels/article/view/246 |
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