Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.

Telomere length (i.e., the length of the repeated sequences of DNA at the end of chromosomes) is a promising indicator of overall stress. Our study aimed to compare the effects of a stress-inducing separation process between dams and their calves, with either a gradual or a nose-flap separation meth...

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Main Authors: Janja Sirovnik, Rebecca Simon, Anina Vogt, Kerstin Barth, Steve Smith, Susanne Waiblinger, Gesine Lühken, Uta König von Borstel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0319156
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author Janja Sirovnik
Rebecca Simon
Anina Vogt
Kerstin Barth
Steve Smith
Susanne Waiblinger
Gesine Lühken
Uta König von Borstel
author_facet Janja Sirovnik
Rebecca Simon
Anina Vogt
Kerstin Barth
Steve Smith
Susanne Waiblinger
Gesine Lühken
Uta König von Borstel
author_sort Janja Sirovnik
collection DOAJ
description Telomere length (i.e., the length of the repeated sequences of DNA at the end of chromosomes) is a promising indicator of overall stress. Our study aimed to compare the effects of a stress-inducing separation process between dams and their calves, with either a gradual or a nose-flap separation method after a three-months dam-calf contact since calving, on relative telomere length (RTL). Due to their nature, the nose-flap and gradual separation method have different effects on behaviour, stress hormone levels and physical development during and after dam-calf separation, which requires an overall measure of the weaning and separation stress during both procedures. We also investigated correlations between behavioural and other physiological stress indicators on RTL. We found no significant effect of the weaning and separation method on RTL in dairy calves after weaning and separation from their dams, but a tendency for shorter RTL in gradually separated dams compared to nose-flap separated dams. No correlations between behavioural and other physiological stress indicators and RTL were found, which may be due to a short interval between the two RTL measurement points. Future studies should aim to analyse the effect of various separation methods over a longer period and preferably include a non-separation group as reference.
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issn 1932-6203
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spelling doaj-art-a8624322b425402ab34f3fb6b460aeb22025-08-20T02:33:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01203e031915610.1371/journal.pone.0319156Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.Janja SirovnikRebecca SimonAnina VogtKerstin BarthSteve SmithSusanne WaiblingerGesine LühkenUta König von BorstelTelomere length (i.e., the length of the repeated sequences of DNA at the end of chromosomes) is a promising indicator of overall stress. Our study aimed to compare the effects of a stress-inducing separation process between dams and their calves, with either a gradual or a nose-flap separation method after a three-months dam-calf contact since calving, on relative telomere length (RTL). Due to their nature, the nose-flap and gradual separation method have different effects on behaviour, stress hormone levels and physical development during and after dam-calf separation, which requires an overall measure of the weaning and separation stress during both procedures. We also investigated correlations between behavioural and other physiological stress indicators on RTL. We found no significant effect of the weaning and separation method on RTL in dairy calves after weaning and separation from their dams, but a tendency for shorter RTL in gradually separated dams compared to nose-flap separated dams. No correlations between behavioural and other physiological stress indicators and RTL were found, which may be due to a short interval between the two RTL measurement points. Future studies should aim to analyse the effect of various separation methods over a longer period and preferably include a non-separation group as reference.https://doi.org/10.1371/journal.pone.0319156
spellingShingle Janja Sirovnik
Rebecca Simon
Anina Vogt
Kerstin Barth
Steve Smith
Susanne Waiblinger
Gesine Lühken
Uta König von Borstel
Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
PLoS ONE
title Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
title_full Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
title_fullStr Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
title_full_unstemmed Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
title_short Relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact.
title_sort relative telomere length in dairy calves and dams undergoing two different methods of weaning and separation after three months of contact
url https://doi.org/10.1371/journal.pone.0319156
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