Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation
(1) T-2 toxin synthesized by <i>Fusarium oxysporum</i> (<i>F. oxysporum</i>) can cause deterioration of dried fish and endanger human health. (2) The molecular mechanism by which antibacterial lipopeptides surfactin inhibited <i>F. oxysporum</i> growth and toxin p...
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2024-11-01
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| author | Qi Deng Xueting Ren Qin Hu Yuehua Pu Lukman Iddrisu Anand Kumar Meifang Hua Jianmeng Liao Zhijia Fang Ravi Gooneratne |
| author_facet | Qi Deng Xueting Ren Qin Hu Yuehua Pu Lukman Iddrisu Anand Kumar Meifang Hua Jianmeng Liao Zhijia Fang Ravi Gooneratne |
| author_sort | Qi Deng |
| collection | DOAJ |
| description | (1) T-2 toxin synthesized by <i>Fusarium oxysporum</i> (<i>F. oxysporum</i>) can cause deterioration of dried fish and endanger human health. (2) The molecular mechanism by which antibacterial lipopeptides surfactin inhibited <i>F. oxysporum</i> growth and toxin production was elucidated by investigating the intracellular ROS production pathway and the subcellular distribution and transcriptional activity of the transcription factor <i>Yap1</i> and its regulation of <i>Tri5</i> gene in <i>F. oxysporum</i>. (3) Surfactin caused hyphal damage and nucleic acid and protein leakage; thus, the growth of <i>F. oxysporum</i> was disrupted. Surfactin’s excessive accumulation of intracellular ROS triggered the translocation of transcription factor <i>Yap1</i> into the nucleus, resulting in toxin cluster gene <i>Tri5</i> expression inhibition, thereby blocking T-2 toxin synthesis. (4) This is a novel mechanism by which surfactin inhibits the growth and T-2 toxin synthesis of <i>F. oxysporum</i> from multiple aspects, including cell structural integrity and the ROS-<i>Yap1</i> signaling pathway. (5) This study provides a theoretical basis for the application of surfactin in the antifungal control of aquatic dry products. |
| format | Article |
| id | doaj-art-a85a065ba5eb40798e0368c98ec3b5ef |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-a85a065ba5eb40798e0368c98ec3b5ef2025-08-20T02:04:55ZengMDPI AGMolecules1420-30492024-11-012922540210.3390/molecules29225402Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS AccumulationQi Deng0Xueting Ren1Qin Hu2Yuehua Pu3Lukman Iddrisu4Anand Kumar5Meifang Hua6Jianmeng Liao7Zhijia Fang8Ravi Gooneratne9Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaDevelopment and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524002, ChinaZhanjiang Institute of Food and Drug Control, Zhanjiang 524022, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Institute of Special Equipment Inspection and Research Zhanjiang Branch, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, P.O. Box 85084, Lincoln 7647, New Zealand(1) T-2 toxin synthesized by <i>Fusarium oxysporum</i> (<i>F. oxysporum</i>) can cause deterioration of dried fish and endanger human health. (2) The molecular mechanism by which antibacterial lipopeptides surfactin inhibited <i>F. oxysporum</i> growth and toxin production was elucidated by investigating the intracellular ROS production pathway and the subcellular distribution and transcriptional activity of the transcription factor <i>Yap1</i> and its regulation of <i>Tri5</i> gene in <i>F. oxysporum</i>. (3) Surfactin caused hyphal damage and nucleic acid and protein leakage; thus, the growth of <i>F. oxysporum</i> was disrupted. Surfactin’s excessive accumulation of intracellular ROS triggered the translocation of transcription factor <i>Yap1</i> into the nucleus, resulting in toxin cluster gene <i>Tri5</i> expression inhibition, thereby blocking T-2 toxin synthesis. (4) This is a novel mechanism by which surfactin inhibits the growth and T-2 toxin synthesis of <i>F. oxysporum</i> from multiple aspects, including cell structural integrity and the ROS-<i>Yap1</i> signaling pathway. (5) This study provides a theoretical basis for the application of surfactin in the antifungal control of aquatic dry products.https://www.mdpi.com/1420-3049/29/22/5402dried fishsurfactin<i>F. oxysporum</i>T-2 toxinROS<i>Yap1</i> |
| spellingShingle | Qi Deng Xueting Ren Qin Hu Yuehua Pu Lukman Iddrisu Anand Kumar Meifang Hua Jianmeng Liao Zhijia Fang Ravi Gooneratne Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation Molecules dried fish surfactin <i>F. oxysporum</i> T-2 toxin ROS <i>Yap1</i> |
| title | Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation |
| title_full | Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation |
| title_fullStr | Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation |
| title_full_unstemmed | Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation |
| title_short | Molecular Mechanism: Inhibition of <i>Fusarium oxysporum</i> T-2 Toxin Synthesis by Surfactin in Dried Fish: Induction of <i>Yap1</i> Nucleation by ROS Accumulation |
| title_sort | molecular mechanism inhibition of i fusarium oxysporum i t 2 toxin synthesis by surfactin in dried fish induction of i yap1 i nucleation by ros accumulation |
| topic | dried fish surfactin <i>F. oxysporum</i> T-2 toxin ROS <i>Yap1</i> |
| url | https://www.mdpi.com/1420-3049/29/22/5402 |
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