First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye
Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Fi...
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| Format: | Article |
| Language: | English |
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Yuzuncu Yil University Faculty of Agriculture
2023-03-01
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| Series: | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
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| _version_ | 1849698713968050176 |
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| author | Mustafa USTA Abdullah GÜLLER Hikmet Murat SİPAHİOĞLU |
| author_facet | Mustafa USTA Abdullah GÜLLER Hikmet Murat SİPAHİOĞLU |
| author_sort | Mustafa USTA |
| collection | DOAJ |
| description | Phytoplasmas are dangerous bacteria severely infecting agricultural
production worldwide. In the present study, the identification of phytoplasmas
infecting tomato plants showing symptoms such as small leaves, flower
abnormalities, stunting, witches' broom, and reddening was performed. Five
plants, two symptomatic and three asymptomatic, were tested to verify
phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a
template for PCR reactions. The phytoplasma agents were confirmed in the two
symptomatic samples. BLASTn search of 16S rRNA of two sequences shared
identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’.
Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is
identical to the reference pattern of the 16SrII-D subgroup, with a similarity
coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic
dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D
subgroup. This is the first report of tomato witches' broom disease related to
16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye. |
| format | Article |
| id | doaj-art-a858aeb7256f4e7ebfbb25728f69dd80 |
| institution | DOAJ |
| issn | 1308-7576 1308-7584 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Yuzuncu Yil University Faculty of Agriculture |
| record_format | Article |
| series | Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi |
| spelling | doaj-art-a858aeb7256f4e7ebfbb25728f69dd802025-08-20T03:18:49ZengYuzuncu Yil University Faculty of AgricultureYüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi1308-75761308-75842023-03-01331546310.29133/yyutbd.1205091First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye Mustafa USTAhttps://orcid.org/0000-0002-3940-2774Abdullah GÜLLERhttps://orcid.org/0000-0003-3887-4208Hikmet Murat SİPAHİOĞLUhttps://orcid.org/0000-0002-2304-2794Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye. 16s rrnamolecular phylogenyphloem restricted bacteriarflpsolanum lycopersicum |
| spellingShingle | Mustafa USTA Abdullah GÜLLER Hikmet Murat SİPAHİOĞLU First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye Yüzüncü Yıl Üniversitesi Tarim Bilimleri Dergisi 16s rrna molecular phylogeny phloem restricted bacteria rflp solanum lycopersicum |
| title | First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye |
| title_full | First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye |
| title_fullStr | First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye |
| title_full_unstemmed | First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye |
| title_short | First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye |
| title_sort | first report of candidatus phytoplasma australasia strain related to witches broom of tomato in turkiye |
| topic | 16s rrna molecular phylogeny phloem restricted bacteria rflp solanum lycopersicum |
| work_keys_str_mv | AT mustafausta firstreportofcandidatusphytoplasmaaustralasiastrainrelatedtowitchesbroomoftomatointurkiye AT abdullahguller firstreportofcandidatusphytoplasmaaustralasiastrainrelatedtowitchesbroomoftomatointurkiye AT hikmetmuratsipahioglu firstreportofcandidatusphytoplasmaaustralasiastrainrelatedtowitchesbroomoftomatointurkiye |