Gut metagenomic features of frailty

This study investigates the relationship between frailty severity and gut microbiome characteristics in adults in Kazakhstan. We analyzed 158 participants across four frailty severity (mild to very severe) using metagenomic sequencing of stool samples. Frailty was significantly correlated with age,...

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Main Authors: Zharkyn Jarmukhanov, Nurislam Mukhanbetzhanov, Elizaveta Vinogradova, Samat Kozhakhmetov, Almagul Kushugulova
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Cellular and Infection Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1486579/full
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author Zharkyn Jarmukhanov
Nurislam Mukhanbetzhanov
Elizaveta Vinogradova
Samat Kozhakhmetov
Almagul Kushugulova
author_facet Zharkyn Jarmukhanov
Nurislam Mukhanbetzhanov
Elizaveta Vinogradova
Samat Kozhakhmetov
Almagul Kushugulova
author_sort Zharkyn Jarmukhanov
collection DOAJ
description This study investigates the relationship between frailty severity and gut microbiome characteristics in adults in Kazakhstan. We analyzed 158 participants across four frailty severity (mild to very severe) using metagenomic sequencing of stool samples. Frailty was significantly correlated with age, weight, and functional measures like walking speed and grip strength. Microbial diversity decreased significantly with increasing frailty. Beta diversity analysis revealed distinct clustering patterns based at phylum level. Taxonomically, we observed a significant inverse correlation between Firmicutes abundance and frailty. Classes like Clostridia and Erysipelotrichia decreased with frailty, while Bacteroidia and Actinobacteria increased. At the family level, Oscillospiraceae showed a positive correlation with frailty. Functionally, we identified significant correlations between frailty measures and specific metabolic pathways. The frailty index negatively correlated with pathways involved in cobalamin, arginine and molybdenum cofactor biosynthesis and positively correlated with folate biosynthesis. Physical performance measures strongly correlated with pathways related to nucleotide biosynthesis, and one-carbon metabolism. We propose these identified features may constitute a “frailty-associated metabolic signature” in the gut microbiome. This signature suggests multiple interconnected mechanisms through which the microbiome may influence frailty development, including modulation of inflammation, alterations in energy metabolism, and potential impacts on muscle function through microbial metabolites.
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spelling doaj-art-a85521ed499748a89df8f4926a543fe72025-08-20T02:33:34ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-11-011410.3389/fcimb.2024.14865791486579Gut metagenomic features of frailtyZharkyn JarmukhanovNurislam MukhanbetzhanovElizaveta VinogradovaSamat KozhakhmetovAlmagul KushugulovaThis study investigates the relationship between frailty severity and gut microbiome characteristics in adults in Kazakhstan. We analyzed 158 participants across four frailty severity (mild to very severe) using metagenomic sequencing of stool samples. Frailty was significantly correlated with age, weight, and functional measures like walking speed and grip strength. Microbial diversity decreased significantly with increasing frailty. Beta diversity analysis revealed distinct clustering patterns based at phylum level. Taxonomically, we observed a significant inverse correlation between Firmicutes abundance and frailty. Classes like Clostridia and Erysipelotrichia decreased with frailty, while Bacteroidia and Actinobacteria increased. At the family level, Oscillospiraceae showed a positive correlation with frailty. Functionally, we identified significant correlations between frailty measures and specific metabolic pathways. The frailty index negatively correlated with pathways involved in cobalamin, arginine and molybdenum cofactor biosynthesis and positively correlated with folate biosynthesis. Physical performance measures strongly correlated with pathways related to nucleotide biosynthesis, and one-carbon metabolism. We propose these identified features may constitute a “frailty-associated metabolic signature” in the gut microbiome. This signature suggests multiple interconnected mechanisms through which the microbiome may influence frailty development, including modulation of inflammation, alterations in energy metabolism, and potential impacts on muscle function through microbial metabolites.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1486579/fullfrailtygut microbiomemetagenomicsmetabolic pathwaysmicrobial diversity
spellingShingle Zharkyn Jarmukhanov
Nurislam Mukhanbetzhanov
Elizaveta Vinogradova
Samat Kozhakhmetov
Almagul Kushugulova
Gut metagenomic features of frailty
Frontiers in Cellular and Infection Microbiology
frailty
gut microbiome
metagenomics
metabolic pathways
microbial diversity
title Gut metagenomic features of frailty
title_full Gut metagenomic features of frailty
title_fullStr Gut metagenomic features of frailty
title_full_unstemmed Gut metagenomic features of frailty
title_short Gut metagenomic features of frailty
title_sort gut metagenomic features of frailty
topic frailty
gut microbiome
metagenomics
metabolic pathways
microbial diversity
url https://www.frontiersin.org/articles/10.3389/fcimb.2024.1486579/full
work_keys_str_mv AT zharkynjarmukhanov gutmetagenomicfeaturesoffrailty
AT nurislammukhanbetzhanov gutmetagenomicfeaturesoffrailty
AT elizavetavinogradova gutmetagenomicfeaturesoffrailty
AT samatkozhakhmetov gutmetagenomicfeaturesoffrailty
AT almagulkushugulova gutmetagenomicfeaturesoffrailty