Interaction between two timing microRNAs controls trichome distribution in Arabidopsis.
The miR156-targeted squamosa promoter binding protein like (SPL) transcription factors function as an endogenous age cue in regulating plant phase transition and phase-dependent morphogenesis, but the control of SPL output remains poorly understood. In Arabidopsis thaliana the spatial pattern of tri...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-04-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004266&type=printable |
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| author | Xue-Yi Xue Bo Zhao Lu-Men Chao Dian-Yang Chen Wen-Rui Cui Ying-Bo Mao Ling-Jian Wang Xiao-Ya Chen |
| author_facet | Xue-Yi Xue Bo Zhao Lu-Men Chao Dian-Yang Chen Wen-Rui Cui Ying-Bo Mao Ling-Jian Wang Xiao-Ya Chen |
| author_sort | Xue-Yi Xue |
| collection | DOAJ |
| description | The miR156-targeted squamosa promoter binding protein like (SPL) transcription factors function as an endogenous age cue in regulating plant phase transition and phase-dependent morphogenesis, but the control of SPL output remains poorly understood. In Arabidopsis thaliana the spatial pattern of trichome is a hallmark of phase transition and governed by SPLs. Here, by dissecting the regulatory network controlling trichome formation on stem, we show that the miR171-targeted lost meristems 1 (LOM1), LOM2 and LOM3, encoding GRAS family members previously known to maintain meristem cell polarity, are involved in regulating the SPL activity. Reduced LOM abundance by overexpression of miR171 led to decreased trichome density on stems and floral organs, and conversely, constitutive expression of the miR171-resistant LOM (rLOM) genes promoted trichome production, indicating that LOMs enhance trichome initiation at reproductive stage. Genetic analysis demonstrated LOMs shaping trichome distribution is dependent on SPLs, which positively regulate trichome repressor genes TRICHOMELESS 1 (TCL1) and TRIPTYCHON (TRY). Physical interaction between the N-terminus of LOMs and SPLs underpins the repression of SPL activity. Importantly, other growth and developmental events, such as flowering, are also modulated by LOM-SPL interaction, indicating a broad effect of the LOM-SPL interplay. Furthermore, we provide evidence that MIR171 gene expression is regulated by its targeted LOMs, forming a homeostatic feedback loop. Our data uncover an antagonistic interplay between the two timing miRNAs in controlling plant growth, phase transition and morphogenesis through direct interaction of their targets. |
| format | Article |
| id | doaj-art-a84f3a56e8b34ab99def0460d8f702af |
| institution | DOAJ |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2014-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-a84f3a56e8b34ab99def0460d8f702af2025-08-20T03:00:29ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-04-01104e100426610.1371/journal.pgen.1004266Interaction between two timing microRNAs controls trichome distribution in Arabidopsis.Xue-Yi XueBo ZhaoLu-Men ChaoDian-Yang ChenWen-Rui CuiYing-Bo MaoLing-Jian WangXiao-Ya ChenThe miR156-targeted squamosa promoter binding protein like (SPL) transcription factors function as an endogenous age cue in regulating plant phase transition and phase-dependent morphogenesis, but the control of SPL output remains poorly understood. In Arabidopsis thaliana the spatial pattern of trichome is a hallmark of phase transition and governed by SPLs. Here, by dissecting the regulatory network controlling trichome formation on stem, we show that the miR171-targeted lost meristems 1 (LOM1), LOM2 and LOM3, encoding GRAS family members previously known to maintain meristem cell polarity, are involved in regulating the SPL activity. Reduced LOM abundance by overexpression of miR171 led to decreased trichome density on stems and floral organs, and conversely, constitutive expression of the miR171-resistant LOM (rLOM) genes promoted trichome production, indicating that LOMs enhance trichome initiation at reproductive stage. Genetic analysis demonstrated LOMs shaping trichome distribution is dependent on SPLs, which positively regulate trichome repressor genes TRICHOMELESS 1 (TCL1) and TRIPTYCHON (TRY). Physical interaction between the N-terminus of LOMs and SPLs underpins the repression of SPL activity. Importantly, other growth and developmental events, such as flowering, are also modulated by LOM-SPL interaction, indicating a broad effect of the LOM-SPL interplay. Furthermore, we provide evidence that MIR171 gene expression is regulated by its targeted LOMs, forming a homeostatic feedback loop. Our data uncover an antagonistic interplay between the two timing miRNAs in controlling plant growth, phase transition and morphogenesis through direct interaction of their targets.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004266&type=printable |
| spellingShingle | Xue-Yi Xue Bo Zhao Lu-Men Chao Dian-Yang Chen Wen-Rui Cui Ying-Bo Mao Ling-Jian Wang Xiao-Ya Chen Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. PLoS Genetics |
| title | Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. |
| title_full | Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. |
| title_fullStr | Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. |
| title_full_unstemmed | Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. |
| title_short | Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. |
| title_sort | interaction between two timing micrornas controls trichome distribution in arabidopsis |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004266&type=printable |
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