In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus
Hydatidosis is characterized by the long-term coexistence of larva Echinococcus granulosus and its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2009/624919 |
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| author | Razika Zeghir-Bouteldja Manel Amri Saliha Aitaissa Samia Bouaziz Dalila Mezioug Chafia Touil-Boukoffa |
| author_facet | Razika Zeghir-Bouteldja Manel Amri Saliha Aitaissa Samia Bouaziz Dalila Mezioug Chafia Touil-Boukoffa |
| author_sort | Razika Zeghir-Bouteldja |
| collection | DOAJ |
| description | Hydatidosis is characterized by the long-term coexistence of larva Echinococcus granulosus and its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity of NO2− and ONOO− 24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated that ONOO− is more cytotoxic than NO2−. In contrast, NO3− has no effect. These results suggest possible involvement of NO2− and ONOO− in antihydatic action and point the efficacy of these metabolites as scolicidal agents. |
| format | Article |
| id | doaj-art-a83c4d6ea1bf4b3580922ebc0f07ddb2 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-a83c4d6ea1bf4b3580922ebc0f07ddb22025-08-20T03:39:09ZengWileyJournal of Parasitology Research2090-00232090-00312009-01-01200910.1155/2009/624919624919In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosusRazika Zeghir-Bouteldja0Manel Amri1Saliha Aitaissa2Samia Bouaziz3Dalila Mezioug4Chafia Touil-Boukoffa5Laboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaLaboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaLaboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaLaboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaLaboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaLaboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, AlgeriaHydatidosis is characterized by the long-term coexistence of larva Echinococcus granulosus and its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity of NO2− and ONOO− 24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated that ONOO− is more cytotoxic than NO2−. In contrast, NO3− has no effect. These results suggest possible involvement of NO2− and ONOO− in antihydatic action and point the efficacy of these metabolites as scolicidal agents.http://dx.doi.org/10.1155/2009/624919 |
| spellingShingle | Razika Zeghir-Bouteldja Manel Amri Saliha Aitaissa Samia Bouaziz Dalila Mezioug Chafia Touil-Boukoffa In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus Journal of Parasitology Research |
| title | In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus |
| title_full | In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus |
| title_fullStr | In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus |
| title_full_unstemmed | In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus |
| title_short | In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus |
| title_sort | in vitro study of nitric oxide metabolites effects on human hydatid of echinococcus granulosus |
| url | http://dx.doi.org/10.1155/2009/624919 |
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