Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis
AIM: Evaluating the preventive efficacy and adjuvant role in therapy of two products containing immunoglobulin Y, ImunoinstantG and Imunoinstant Anti-Candida albicans, in a murine model of vaginal candidiasis. BACKGROUND: Vaginal infections caused by Candida albicans affect approximately 75% of wome...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524003795 |
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| author | Mihai Mares Andra Cristina Bostanaru Iliescu Bogdan Minea Robert Capota Dana Ciausu Sliwa Valentin Nastasa |
| author_facet | Mihai Mares Andra Cristina Bostanaru Iliescu Bogdan Minea Robert Capota Dana Ciausu Sliwa Valentin Nastasa |
| author_sort | Mihai Mares |
| collection | DOAJ |
| description | AIM: Evaluating the preventive efficacy and adjuvant role in therapy of two products containing immunoglobulin Y, ImunoinstantG and Imunoinstant Anti-Candida albicans, in a murine model of vaginal candidiasis. BACKGROUND: Vaginal infections caused by Candida albicans affect approximately 75% of women, with recurrence involving immune imbalances in 5-8% of cases. METHODS: Thirty female Balb/C mice were equally divided into three groups: Control (C), ImunoinstantG (IG), and Imunoinstant Anti-Candida albicans (IA). Over 22 days, each experimental group received a dose/kg equivalent to an adult human weighing 70 kg (0.9 mg IgY/mouse in 0.35 mL) administered via gavage. The control group received the same volume of drinking water. Both products were administered preventively, starting 7 days before infection. Infection was induced by intravaginally inoculation of 20 µL suspension of Candida albicans wild-type SC5314, containing 2 × 10⁵ CFU. The fungal load (CFU/mL) was assessed in vaginal lavage on days 5, 10, and 15 post-infection. RESULTS: At 15 days post-infection, the fungal load was 2.1 × 10⁴ CFU/mL in group C, 0.4 × 10³ CFU/mL in group IG, and 0.9 × 10² CFU/mL in group IA, respectively. The reduction rate of the fungal load was highest in group IA at all evaluation points, reaching 99.6%. Cytological examination of the lavage fluid indicated a reduced presence of filamentous forms of the yeast and less inflammation for both ImunoinstantG and Imunoinstant Anti-Candida albicans treatments. CONCLUSIONS: These products demonstrated real preventive and therapeutic qualities, based on the significant and gradual reduction in vaginal fungal load throughout the administration period. |
| format | Article |
| id | doaj-art-a83c0dde7e0044b6861847d68e392ca7 |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-a83c0dde7e0044b6861847d68e392ca72025-08-20T01:57:48ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-01396310.1016/j.jgar.2024.10.202Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasisMihai Mares0Andra Cristina Bostanaru Iliescu1Bogdan Minea2Robert Capota3Dana Ciausu Sliwa4Valentin Nastasa5Iasi University of Life Sciences, Iasi, RomaniaIasi University of Life Sciences, Iasi, RomaniaGr. T. Popa University of Medicine and Pharmacy, Iasi, RomaniaIasi University of Life Sciences, Iasi, RomaniaIasi University of Life Sciences, Iasi, RomaniaIasi University of Life Sciences, Iasi, RomaniaAIM: Evaluating the preventive efficacy and adjuvant role in therapy of two products containing immunoglobulin Y, ImunoinstantG and Imunoinstant Anti-Candida albicans, in a murine model of vaginal candidiasis. BACKGROUND: Vaginal infections caused by Candida albicans affect approximately 75% of women, with recurrence involving immune imbalances in 5-8% of cases. METHODS: Thirty female Balb/C mice were equally divided into three groups: Control (C), ImunoinstantG (IG), and Imunoinstant Anti-Candida albicans (IA). Over 22 days, each experimental group received a dose/kg equivalent to an adult human weighing 70 kg (0.9 mg IgY/mouse in 0.35 mL) administered via gavage. The control group received the same volume of drinking water. Both products were administered preventively, starting 7 days before infection. Infection was induced by intravaginally inoculation of 20 µL suspension of Candida albicans wild-type SC5314, containing 2 × 10⁵ CFU. The fungal load (CFU/mL) was assessed in vaginal lavage on days 5, 10, and 15 post-infection. RESULTS: At 15 days post-infection, the fungal load was 2.1 × 10⁴ CFU/mL in group C, 0.4 × 10³ CFU/mL in group IG, and 0.9 × 10² CFU/mL in group IA, respectively. The reduction rate of the fungal load was highest in group IA at all evaluation points, reaching 99.6%. Cytological examination of the lavage fluid indicated a reduced presence of filamentous forms of the yeast and less inflammation for both ImunoinstantG and Imunoinstant Anti-Candida albicans treatments. CONCLUSIONS: These products demonstrated real preventive and therapeutic qualities, based on the significant and gradual reduction in vaginal fungal load throughout the administration period.http://www.sciencedirect.com/science/article/pii/S2213716524003795Candida albicansImmunoglobulin Yvaginal candidiasis |
| spellingShingle | Mihai Mares Andra Cristina Bostanaru Iliescu Bogdan Minea Robert Capota Dana Ciausu Sliwa Valentin Nastasa Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis Journal of Global Antimicrobial Resistance Candida albicans Immunoglobulin Y vaginal candidiasis |
| title | Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| title_full | Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| title_fullStr | Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| title_full_unstemmed | Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| title_short | Hyperimmune egg-based IgY-rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| title_sort | hyperimmune egg based igy rich formulations as adjuvant therapy in a murine model of vaginal candidiasis |
| topic | Candida albicans Immunoglobulin Y vaginal candidiasis |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524003795 |
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