Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences
The nematode Metaparasitylenchus hypothenemi (Poinar) (Tylenchida: Allantonematidae) is a parasite of the coffee berry borer (Hypothenemus hampei), one of the most significant pests affecting coffee crops. This study aimed to analyze the genetic variation of M. hypothenemi using the mitochondrial c...
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Universidad Nacional Autónoma de México
2025-08-01
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| Series: | Revista Mexicana de Biodiversidad |
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| Online Access: | https://revista.ib.unam.mx/index.php/bio/article/view/5460 |
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| author | Marina Simota-Ruiz Oscar Mikery Juan Cisneros Oscar Carmona-Castro Alfredo Castillo-Vera Alma E. García-Morales |
| author_facet | Marina Simota-Ruiz Oscar Mikery Juan Cisneros Oscar Carmona-Castro Alfredo Castillo-Vera Alma E. García-Morales |
| author_sort | Marina Simota-Ruiz |
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The nematode Metaparasitylenchus hypothenemi (Poinar) (Tylenchida: Allantonematidae) is a parasite of the coffee berry borer (Hypothenemus hampei), one of the most significant pests affecting coffee crops. This study aimed to analyze the genetic variation of M. hypothenemi using the mitochondrial cytochrome oxidase subunit I (COI) gene. Reproductive females of M. hypothenemi were extracted from parasitized wild coffee berry borers
for DNA extraction, amplification, and sequencing. Phylogenetic analyses revealed 2 well-differentiated lineages and 6 haplotypes distributed across 18 populations along a 100 km transect. Global genetic diversity was moderate (Hd = 0.52 ± 0.06), with a dominant haplotype present in 16 populations and others being representative or geographically isolated. Populations showed high genetic differentiation and restricted gene flow. These findings confirm the utility of the COI gene for analyzing the genetic variation of M. hypothenemi and provide a molecular basis for future studies on its biology and management.
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| format | Article |
| id | doaj-art-a82fc32e98f540a9930725838476bb0f |
| institution | Kabale University |
| issn | 1870-3453 2007-8706 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Universidad Nacional Autónoma de México |
| record_format | Article |
| series | Revista Mexicana de Biodiversidad |
| spelling | doaj-art-a82fc32e98f540a9930725838476bb0f2025-08-20T03:36:34ZengUniversidad Nacional Autónoma de MéxicoRevista Mexicana de Biodiversidad1870-34532007-87062025-08-019610.22201/ib.20078706e.2025.96.5460Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequencesMarina Simota-Ruiz0Oscar Mikery1Juan Cisneros 2Oscar Carmona-Castro3Alfredo Castillo-Vera4Alma E. García-Morales5El Colegio de la Frontera Sur, Unidad Tapachula, ChiapasOrtomolecular Mexicana BC El Colegio de la Frontera Sur, Unidad Tapachula, Chiapas El Colegio de la Frontera Sur, Unidad Tapachula, Chiapas El Colegio de la Frontera Sur, Unidad Tapachula, ChiapasEl Colegio de la Frontera Sur, Unidad Chetumal, Quintana Roo The nematode Metaparasitylenchus hypothenemi (Poinar) (Tylenchida: Allantonematidae) is a parasite of the coffee berry borer (Hypothenemus hampei), one of the most significant pests affecting coffee crops. This study aimed to analyze the genetic variation of M. hypothenemi using the mitochondrial cytochrome oxidase subunit I (COI) gene. Reproductive females of M. hypothenemi were extracted from parasitized wild coffee berry borers for DNA extraction, amplification, and sequencing. Phylogenetic analyses revealed 2 well-differentiated lineages and 6 haplotypes distributed across 18 populations along a 100 km transect. Global genetic diversity was moderate (Hd = 0.52 ± 0.06), with a dominant haplotype present in 16 populations and others being representative or geographically isolated. Populations showed high genetic differentiation and restricted gene flow. These findings confirm the utility of the COI gene for analyzing the genetic variation of M. hypothenemi and provide a molecular basis for future studies on its biology and management. https://revista.ib.unam.mx/index.php/bio/article/view/5460Obligate endoparasite Insect pestGenetic variationCOI GenePhylogenetic treeGenetic structure |
| spellingShingle | Marina Simota-Ruiz Oscar Mikery Juan Cisneros Oscar Carmona-Castro Alfredo Castillo-Vera Alma E. García-Morales Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences Revista Mexicana de Biodiversidad Obligate endoparasite Insect pest Genetic variation COI Gene Phylogenetic tree Genetic structure |
| title | Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences |
| title_full | Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences |
| title_fullStr | Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences |
| title_full_unstemmed | Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences |
| title_short | Genetic variability of the nematode Metaparasitylenchus hypothenemi (Tylenchida: Allantonematidae), natural parasite of the coffee berry borer (Coleoptera: Curculionidae: Scolytinae), using COI sequences |
| title_sort | genetic variability of the nematode metaparasitylenchus hypothenemi tylenchida allantonematidae natural parasite of the coffee berry borer coleoptera curculionidae scolytinae using coi sequences |
| topic | Obligate endoparasite Insect pest Genetic variation COI Gene Phylogenetic tree Genetic structure |
| url | https://revista.ib.unam.mx/index.php/bio/article/view/5460 |
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