Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra

ABSTRACT Soil microbiota can colonise plant roots through a two‐step selection process, involving recruitment of microbiota first from bulk soil into plant rhizospheres, then into root endospheres. This process is poorly understood in all but a few model species (e.g., Arabidopsis), which is surpris...

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Main Authors: Riley J. Hodgson, Christian Cando‐Dumancela, Craig Liddicoat, Sunita A. Ramesh, Robert A. Edwards, Martin F. Breed
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71595
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author Riley J. Hodgson
Christian Cando‐Dumancela
Craig Liddicoat
Sunita A. Ramesh
Robert A. Edwards
Martin F. Breed
author_facet Riley J. Hodgson
Christian Cando‐Dumancela
Craig Liddicoat
Sunita A. Ramesh
Robert A. Edwards
Martin F. Breed
author_sort Riley J. Hodgson
collection DOAJ
description ABSTRACT Soil microbiota can colonise plant roots through a two‐step selection process, involving recruitment of microbiota first from bulk soil into plant rhizospheres, then into root endospheres. This process is poorly understood in all but a few model species (e.g., Arabidopsis), which is surprising given its fundamental role in plant and soil ecology. Here, we examined the microbial community assembly processes across the rhizospheres and root endospheres in eight natural populations of the pan‐palaeotropical C4 grass, Themeda triandra, in southern Australia. Using a space‐for‐time substitution approach, we assessed whether bacterial root colonisation patterns conformed to the two‐step model and tested whether community assembly was driven more by deterministic or stochastic processes. Our results show that the two‐step selection process shaped bacterial recruitment dynamics across these natural T. triandra populations, and we provide clear evidence that host plants influence microbial assembly via deterministic pressures that produce strong community convergence within endospheres. These findings highlight the central role of host filtering in shaping a conserved ‘core’ endosphere microbiome. However, limited understanding of these endosphere communities constrains efforts to harness these important relationships to, for example, improve plant propagation and revegetation practices.
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spelling doaj-art-a8279066f2044457bb07b4e970dd2b2a2025-08-20T02:43:39ZengWileyEcology and Evolution2045-77582025-06-01156n/an/a10.1002/ece3.71595Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandraRiley J. Hodgson0Christian Cando‐Dumancela1Craig Liddicoat2Sunita A. Ramesh3Robert A. Edwards4Martin F. Breed5College of Science and Engineering Flinders University Bedford Park South Australia AustraliaCollege of Science and Engineering Flinders University Bedford Park South Australia AustraliaCollege of Science and Engineering Flinders University Bedford Park South Australia AustraliaCollege of Science and Engineering Flinders University Bedford Park South Australia AustraliaCollege of Science and Engineering Flinders University Bedford Park South Australia AustraliaCollege of Science and Engineering Flinders University Bedford Park South Australia AustraliaABSTRACT Soil microbiota can colonise plant roots through a two‐step selection process, involving recruitment of microbiota first from bulk soil into plant rhizospheres, then into root endospheres. This process is poorly understood in all but a few model species (e.g., Arabidopsis), which is surprising given its fundamental role in plant and soil ecology. Here, we examined the microbial community assembly processes across the rhizospheres and root endospheres in eight natural populations of the pan‐palaeotropical C4 grass, Themeda triandra, in southern Australia. Using a space‐for‐time substitution approach, we assessed whether bacterial root colonisation patterns conformed to the two‐step model and tested whether community assembly was driven more by deterministic or stochastic processes. Our results show that the two‐step selection process shaped bacterial recruitment dynamics across these natural T. triandra populations, and we provide clear evidence that host plants influence microbial assembly via deterministic pressures that produce strong community convergence within endospheres. These findings highlight the central role of host filtering in shaping a conserved ‘core’ endosphere microbiome. However, limited understanding of these endosphere communities constrains efforts to harness these important relationships to, for example, improve plant propagation and revegetation practices.https://doi.org/10.1002/ece3.71595endospheremicrobial ecologyneutral theory modelrhizosphereThemeda triandratwo‐step selection process
spellingShingle Riley J. Hodgson
Christian Cando‐Dumancela
Craig Liddicoat
Sunita A. Ramesh
Robert A. Edwards
Martin F. Breed
Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
Ecology and Evolution
endosphere
microbial ecology
neutral theory model
rhizosphere
Themeda triandra
two‐step selection process
title Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
title_full Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
title_fullStr Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
title_full_unstemmed Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
title_short Strong Host Modulation of Rhizosphere‐to‐Endosphere Microbial Colonisation in Natural Populations of the Pan‐Palaeotropical Keystone Grass Species, Themeda triandra
title_sort strong host modulation of rhizosphere to endosphere microbial colonisation in natural populations of the pan palaeotropical keystone grass species themeda triandra
topic endosphere
microbial ecology
neutral theory model
rhizosphere
Themeda triandra
two‐step selection process
url https://doi.org/10.1002/ece3.71595
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