Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera
Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/285 |
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| author | Ye. S. Kostrova O. P. Bjadovskaya Ye. V. Peshkova O. V. Pruntova |
| author_facet | Ye. S. Kostrova O. P. Bjadovskaya Ye. V. Peshkova O. V. Pruntova |
| author_sort | Ye. S. Kostrova |
| collection | DOAJ |
| description | Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined. |
| format | Article |
| id | doaj-art-a820dc253ac7435c928fc627677ac32b |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-a820dc253ac7435c928fc627677ac32b2025-02-06T09:52:06ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01013437285Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine seraYe. S. Kostrova0O. P. Bjadovskaya1Ye. V. Peshkova2O. V. Pruntova3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirIndirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.https://veterinary.arriah.ru/jour/article/view/285mannheimia haemolyticaиммуноферментный анализлипополисахаридный антигенкрупный рогатый скотmannheimia haemolyticaenzyme-linked immunosorbent assaylipopolysaccharide antigencattle |
| spellingShingle | Ye. S. Kostrova O. P. Bjadovskaya Ye. V. Peshkova O. V. Pruntova Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera Ветеринария сегодня mannheimia haemolytica иммуноферментный анализ липополисахаридный антиген крупный рогатый скот mannheimia haemolytica enzyme-linked immunosorbent assay lipopolysaccharide antigen cattle |
| title | Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera |
| title_full | Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera |
| title_fullStr | Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera |
| title_full_unstemmed | Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera |
| title_short | Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera |
| title_sort | design of indirect elisa based test system for determination of mannheimia haemolytica antibody titre in bovine sera |
| topic | mannheimia haemolytica иммуноферментный анализ липополисахаридный антиген крупный рогатый скот mannheimia haemolytica enzyme-linked immunosorbent assay lipopolysaccharide antigen cattle |
| url | https://veterinary.arriah.ru/jour/article/view/285 |
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